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Guo-hua Li

Publications and source records attributed to Guo-hua Li.

10 recordsLinked to original sources
PubMed2005

[Seroprevalence of antibody against human metapneumovirus in Beijing].

OBJECTIVE: To understand the seroprevalence of antibody against the newly identified human metapneumovirus (hMPV) in Beijing. METHODS: The antigenic specificity of hMPV N protein cloned into vector pET30a and then expressed in E coli was verified by using SDS-PAGE and Western blotting in 116 serum specimens. The plasmid pET30a without insert was used as control. Totally 710 serum specimens collected from non-respiratory infection patients visited the Outpatient Departments of Children's Hospital affiliated to Capital Institute of Pediatrics and Xuanwu Hospital, Beijing from April 1996 to March 1997 were tested for specific IgG antibody against hMPV N protein. RESULTS: The bands with expected molecular weight showed only on the membranes transferred by the expressed hMPV N protein and incubated with rabbit hyperimmune serum against hMPV N protein polypeptides as well as the collected human sera, indicating the specificity of the expressed hMPV N protein. Out of 710 specimens tested, 17.2% (122/710) were positive for antibody to N protein. Antibody positive rate was the lowest in 2 to 6 months old infants (3.1%); the rate declined from 13.2% in newborns to 6.1% in 1 to 2 months old infants, then to 3.1% in the 2 to 6 months group, and sustained at about 3.0% from 6 months group to 30 years of age, then increased to 28.1% in 30 to 39-year-old adults, 32.3% in 40 to 49-year-old adults and to 38.5% in the group over age of 50 years. CONCLUSION: The expressed hMPV N protein is reliable when it was used as antigen for testing specific IgG antibody against hMPV in human sera. The high seroprevalence of antibody against hMPV N protein and early age antibody acquisition suggest that hMPV has been circulating in Beijing and the importance of the virus as pathogen should be further analyzed.

Adolescent↗
PubMed2004

[Molecular epidemiologic survey of rotaviruses from infants and children with diarrhea in Shanghai].

OBJECTIVE: To investigate molecular epidemiologic features of rotaviruses circulating in Shanghai, China. METHODS: Stool samples were collected from 1230 hospitalized children with community-acquired and nosocomially acquired diarrhea in Children's Hospital Affiliated to Fudan University between November 1, 1999 and December 31, 2001. Polyacrylamide gel electrophoresis (PAGE) was used to detect rotavirus genomic RNA and identify electropherotypes of group A rotavirus RNAs. Reverse transcription polymerase chain reaction (RT-PCR) was performed to amplify full length VP7 gene and dot blot hybridization was performed to identify rotavirus G serotypes using digoxigenin-labelled variable regions from VP7 genes as probes. These probes were amplified by PCR from recombinant plasmids containing full length G1, G2, G3 and G4 VP7 genes from rotavirus field strains detected in Beijing and digoxigenin labelled dUTP was integrated into the PCR products. The Kruskal-Wallis analysis of variance was employed to analyze whether there were significant differences in variables. RESULTS: Out of 1230 samples investigated, 493 (40.1%) were group A rotavirus gene positive by PAGE, among which 397 (80.5%) showed long electropherotypes, 55 (11.2%) showed short electropherotypes, 18 (3.7%) showed mixed electropherotypes which suggested that the children were co-infected by rotaviruses with different electropherotypes, 23 (4.7%) were non-typable because of degradation of some of the genomic RNA fragments. No group B or group C rotavirus was found. RT-PCRs were performed for 328 fecal specimens containing sufficient rotavirus RNAs and VP7 gene products were obtained from 254 (77.4%) samples. Dot blot hybridization showed serotype G1 accounted for 55.5% (141) of these samples, serotype G3 accounted for 27.6% (70), serotype G2 accounted for 9.4% (24), co-infection by 2 rotaviruses with different G types accounted for 6.3% (16), only 1 G4 was detected and 2 were non-typable. The genomic RNA patterns of all G2 strains were short and those of G1, G3 and G4 strains were long. There were no statistically significant differences for age distribution and clinical manifestations among those infants and children infected by rotaviruses with different G serotypes. CONCLUSION: Group A rotavirus is the major pathogen for diarrhea in infants and children in Shanghai during the period of Nov. 1999 to Dec. 2001. Rotaviruses with long electropherotype were dominant during these years. Serotypes G1 to G3 constituted 98.8% of all 254 strains tested, and G1 was the most common serotype followed by G3 and G2, whereas serotype G4 was seldom found. Some of the children were co-infected by rotaviruses with different G serotypes. Clinical manifestations were not related to the infecting rotavirus with different G serotypes.

Age Factors↗
PubMed2004

[X-ray diffraction quantitative analysis with polynomial-fit method for polycrystalline materials].

The polynomial-fit method for XRD quantitative analysis of polycrystalline materials is presented in this paper, which combines a mathematic function model with computer technology. Based on the construction of diffraction peak mathematic function model, the XRD atlases from experiments were analyzed by means of polynomial whole pattern fitting to the spectral lines using computer software, then the integral intensities of every peak and weight percentages of each phase could be obtained accurately. This paper mainly includes three parts: 1. According to the fact that the diffraction atlases of mixture are weighted superposition of the powder diffraction atlases of each component and the weight factor of each component is relevant to its volume fraction or weight percentage, the theory of polynomial whole pattern fitting is constructed and the weight factor and weight percentage can be worked out. 2. Describe the method and procedure of data acquisition and analysis. 3. Discuss the result of quantitative analysis. The polynomial-fit method not only simplifies the process of data handling, but also increases the accuracy of analytical result.

Algorithms↗
PubMed2003

[Mastoscopic axillary lymph node dissection for the patients with breast cancer].

OBJECTIVE: To analyze the clinical effects of mastoscopic axillary lymph node dissection for the patients with breast cancer. METHODS: Following the liposuction of the axilla, axillary lymph node dissection was performed in 86 breast cancer patients by mastoscopy. RESULTS: The median duration of operation was 55.3 min. The operation time was obviously shorter for the last 76 patients than for the first 10 patients (P < 0.001). The bleeding amount in operation was little, and no patient was intraoperatively transferred to routine open axillary lymph node dissection due to uncontrolled bleeding. A mean number of 15.3 lymph nodes were harvested. 37 patients showed involved lymph nodes. The mean number of involved lymph nodes in these patients was 3.6. There was no intra- and post-operation morbidity. After a median follow-up time of 10.4 months, no axillary relapse or trocar site implantation had occurred. CONCLUSION: Mastoscopic axillary lymph node dissection (MALND) based on the liposuction of axilla is a minimally invasive and clinical feasible procedure. It manifests the functional and cosmetic effects and satisfies the breast cancer patients to certain extent.

Adult↗
PubMed2003

[Mastoscopic extirpation of benign breast masses by small and concealing incision].

OBJECTIVE: To investigate the feasibility and effect of the mastoscopic extirpation of benign breast masses by small and concealing incision. METHODS: 72 benign breast masses of 64 patients were mastoscopically extirpated. Transaxillary and transareolar small incisions were selected under local and vein analgesia anaesthesia. RESULTS: Most of patients were operated in clinic, only 28 patients in hospital. The average operation time was 46.1 minutes. The sensation and erection of breast nipples were normal in all patients. 2 patients with the more obvious complications (one with skin burned by electric scalpel, another with wide subcutaneous ecchymosis) complained this kind of operation, other patients were greatly satisfied with the cosmetic outcome of the operation. CONCLUSIONS: The mastoscopic extirpation of benign breast masses by small and concealing incision can be completed in most of the patients under local anesthesia. The incision is small and concealing, which raises the quality of life of the patients. This operation procedure meets the needs of wide women on the treatment of breast disease to a great extent and also alters the routine conception of the excision of benign breast mass.

Adolescent↗
PubMed2003

[Study on the epidemiological characteristics of severe acute respiratory syndrome in Shanxi province].

OBJECTIVE: To describe the epidemiological characteristics and related factors of SARS in Shanxi in order to provide scientific basis for prevention and control of severe acute respiratory syndrome (SARS). METHODS: Data on clinically-diagnosed SARS cases reported to Shanxi Center for Disease Control and Prevention through SARS reporting system of Shanxi province and epidemiological reports were collected from early March to 20 May, 2003. The characteristics of SARS distribution in time, place and population in Shanxi were described. The epidemiological characteristics and related influential factors were analyzed with EPI info 6.0 software. RESULTS: Since the first imported SARS case was diagnosed clinically on 7 March and till 20 May in Shanxi province, the number of cumulative clinically-diagnosed SARS cases were 445 with an attack rate of 1.34/10,000. 20 deaths occurred in that period with the mortality rate 4.49%. The number of cases increased from 28 March and formed the first peak. However, the number continued to increase until 18 April to have formed the second peak. Since then, the number of cases has gradually decreased gradually. Since 19 May, there has been no clinically-diagnosed cases being reported. SARS cases were mostly seen in urban areas of the city (83.82% of the total SARS cases) with sporadic cases found in rural areas. Students and medical staff and people from 20 - 59 years of age occupied the large part of the cases. Age specific mortality rate increased with age and the male/female ratio was 1:0.87. CONCLUSION: In Shanxi province, the SARS epidemic seemed to have had the following stages: importation of the first case, gradual increase of the number of cases to reach the peak and decreasing. Case identification at early stage as well as taking measures to decrease the chance of transmission were strategically crucial for controlling the spread of SARS virus in the community.

China↗
PubMed2003

[The study of the behavior of exciton and photon within semiconductor microcavity under hydrostatic pressure].

We studied, for the first time, the strong coupling between exciton and cavity mode within semiconductor microcavity under hydrostatic pressure, and measured the Rabi splitting. The strong coupling between exciton and cavity mode, and so Rabi splitting appear clearly as the applied pressure reaches 0.37-0.41 GPa. The experiment result shows that hydrostatic pressure not only can tune the coupling between exciton and cavity mode effectively, but also can keep exciton property almost unchanged during the whole tuning procedure in contrast to other tuning method (temperature field et al). Our result agrees with the related theory very well. The Rabi splitting, extracted from fitting the measured mode-energy vs pressure curves with correspending theoretical model, is equal to 6 meV.

Chemical Phenomena↗
PubMed2002

[A case-control study on risk factors for advanced schistosomiasis japonica].

OBJECTIVE: To investigate risk factors for the development of advanced schistosomiasis. METHODS: The case-control study was designed with a match of 1:1 and 1:2. Healthy persons and chronic schistosomiasis patients were used as control. Each group was composed of 213 cases. Items investigated included: history of schistosomiasis and treatment, exposure to the contaminated water, social-economical condition and nutritional status, other concomitant diseases. Cellular and humoral immunity, HBVMs and ABO blood groups were also detected. Statistical analysis was performed by 1:1 and 1:2 matched single and stepwise conditional logistic regression analysis with SAS software. RESULTS: By stepwise conditional logistic regression analysis, it was revealed that number of schistosomiasis examination (OR = 1.168-1.311), interval from first infection to last treatment(OR = 1.142), interval from first infection to this investigation (OR = 1.089), HBsAg+ (OR = 4.683-10.759), HBcAb+ (OR = 2.873), HBsAg+ + HBeAb+ + HBcAb+ (OR = 7.64) were risk factors of developing advanced schistosomiasis. The average living space and cellular immunity were lower in advanced schistosomiasis patients than others. No association was found between advanced schistosomiasis and ABO blood groups. CONCLUSION: The development of advanced schistosomiasis is associated with repeated infections, and delayed or incomplete treatment. Combined infection with hepatitis B, poor socio-economic conditions and impaired cellular immunity may increase the pathogenetic risk of schistosomiasis.

ABO Blood-Group System↗