PubMed Health⌕ Search

Biomedical subjects

Guo-qing Liu

Publications and source records attributed to Guo-qing Liu.

11 recordsLinked to original sources
PubMed2006

[One stage repairing operation in neonatal hypospadias].

OBJECTIVE: To explore the feasibility of one stage repairing operation on hypospadias in neonatal. METHODS: Sixteen newborn infants with congenital hypospadias dated from May 1998 to Jun. 2004, who was 1 to 29 days old with average 13 days, were performed one stage repairing operation, among whom hypospadias were classified: 4 cases of type I hypospadias, 8 cases of type II, 3 cases of type III and 1 case of type IV. RESULTS: Fourteen cases were cured, 1 case had urethral stricture, and 1 case had fistula. The cure rate of one stage operation was 87.5% (14/16). CONCLUSION: On the premise of the anesthetic safety, one stage hypospadias repairing operation is feasible in some selective cases in neonate.

Humans↗
PubMed2006

[Relevant factors of lymphatic metastasis in rectal carcinoma].

OBJECTIVE: To investigate the rules of lymphatic metastasis of rectal carcinoma, and to help clinical diagnosis and treatment. METHODS: A retrospective analysis was performed in the 979 patients with rectal carcinoma who underwent surgical resection from 1995 to 2004. The associations between lymphatic metastasis and clinicopathologic variables were evaluated by Chi-squared test and logistic regression. RESULTS: The rate of lymph node metstasis was 71.4% for patients younger than 30 years old, 40.7% in the patients with tumor diameters over 6 centimeters, 82.5% in the patients with extraneous tumor invasion, 71.6% for patients of poor-differentiated adenocarcinoma, 70.4% for patients with mucoid adenocarcinoma, 100% for patients with signet-ring cell carcinoma and 46.4% for patients with more than half intestinal circumference invasion. Logistic regression analysis showed that the degree of lymphatic metastasis was related to the differentiating degrees, depths of tumor invasion and intestinal circumference invasion, and the differentiating degree was the major factor. CONCLUSION: The lymphatic metastasis of rectal carcinoma is related to age, tumor size, intestinal circumference invasion, depth of tumor invasion and the differentiating degree of the tumor; the differentiating degree is the major factor.

Adenocarcinoma↗
PubMed2006

[Effects of paeoniflorin on cerebral energy metabolism, nitric oxide and nitric oxide synthase after cerebral ischemia in mongoliagerbils].

OBJECTIVE: To explore the effects of paeoniflorin on antagonising the delayed neuronal death (DND) induced by cerebral ischemia,and the relation between DND, cerebral tissue energy metabolism, nitric oxide (NO) and nitric oxide synthase (NOS). METHOD: Incomplete cerebral ischemia induced was induced by ligating bilateral arteries carotis communis for 20 min followed by reperfusion 48 h in rats. The indexes including Na(+)-K(+)-ATPase activity, lactic acid content, Ca(2+)-ATPase, Mg(2+)-ATPase activity, NO content and NOS activity were determined in fore brain cortex at 48 h after reperfusion. RESULT: Na(+)-K(+)-ATPase, Ca(2+)-ATPase and Mg(2+)-ATPase activity were lowered (P < 0.01), NO level was decreased (P < 0.01), NOS activity dropped (P < 0.01) in cerebral tissue at 48h after reperfusion, but lactic acid level had no change. Paeoniflorin could prevent reduction of Na(+)-K(+)-ATPase activity (P < 0.05, P < 0.01), increase NO level (P < 0.01), enhance NOS activity (P < 0.01) at 48h after reperfusion. CONCLUSION: DND induced by ischemia may be concerned with energy metabolism disorder and decrease of NO formation. Paeoniflorin may play the role of antagonising cerebral ischemia by adjusting cerebral energy metabolism and nitric oxide formation.

Animals↗
PubMed2005

[Over one hundred year sedimentary record of polycyclic aromatic hydrocarbons in the Pearl River Estuary, South China].

The western shoal of the Lingding Bay is known to be an important deposition zone of terrestrial substance in the Pearl River Estuary (PRE). In this study, the vertical variations of concentration and fluxes of polycyclic aromatic hydrocarbon (PAH) are investigated in a core from the west shoal of Lingding Bay. In combining with 210Pb-dating, the PAHs sedimentary record in the last 100 years is reconstructed. The sigmaPAH concentration ranged from 59 ng x g(-1) to 330 ng x g(-1) throughout the core with two distinct peaks. An initial increase in sigmaPAH concentration was found around the 1860s, followed with the first maximum in the 1950s. There was a decrease in total PAHs concentration and flux in 1960s and 1970s. A sharp increase in PAHs levels was observed from 1980s and a maximum was found in the 1990s. PAHs diagnostic ratios indicate that PAH in the sediment core are mainly of pyrolytic origin. PAHs concentrations are found to correlate positively with the Gross Domestic Production, vehicle numbers and power generation in the surrounding regions, indicating that the PAHs in the sediment core are mainly anthropogenic. Atmospheric deposition and land runoff may serve as the important pathway of PAHs input to the sediment.

China↗
PubMed2004

Doxepin protects cultured neurons against oxidative stress-induced injury.

AIM: To investigate protective effects of doxepin (Dox) on cultured neuronal injury induced by oxidative stress. METHODS: Cytotoxicities of glutamate (Glu 0.5 mmol/L, 15 min), sodium dithionite (0.5 mmol/L, 24 h) or hemoglobin (Hb100 mg/L,24 h)and the protective effects of Dox were observed. RESULTS: Exposure of cultured neurons to Glu, sodium dithionite and Hb developed a neurotoxicity expressed in the thiazol blue tetrazolium bromide (MTT) assay, the increase of lactate dehydrogenase (LDH) leakage, malondialdehyde (MDA) content and intracellular [Ca2+]i accumulation, as well as the decrease of superoxide dismutase (SOD) activity. DOX 1-100 nmol/L significantly inhibited all above changes. CONCLUSION: Dox protects cultured neurons against oxidative stress-induced injury by suppressing intracellular [Ca2+]i accumulation, decreasing lipid peroxide generation and stimulating antioxidant enzyme.

Animals↗
PubMed2004

[Effect of fast-acting spray for heat stroke in enhancing heat tolerance of rats].

OBJECTIVE: To observe the effect of fast-acting spray for heat stroke, a preparation of traditional Chinese drugs, in enhancing the heat tolerance in rats. METHODS: Thirty SD rats were randomized into 3 groups(with 10 in each group), and group A was treated with the fast-acting spray for heat stroke, group B with chlorpromazine, and group C with cool boiled water, before they were exposed to heat at 41 degrees Celsius in a heat chamber with relative humidity of 70% till death. The rats' rectal temperature was measured before and 1 h after the heat exposure, and the time when death occurred in the rats was recorded. RESULT: In comparison with group C, the rats in groups A and B had lower rectal temperature and prolonged survival time with decrease mortality. CONCLUSION: The fast-acting spray for heat stroke we prepared may enhance the heat tolerance of rats.

Animals↗
PubMed2004

[Reversal of multidrug resistance by lomerizine in K562/ADM cells].

AIM: To study the effect of lomerizine (Lom) on the reversal of multidrug resistance (MDR) in K562/ADM cells and its mechanism. METHODS: MTT assay was used to determine the influence of Lom on the cytotoxicity of adriamycin (ADM). The effect of Lom on the apoptosis induced by ADM and vincristine (VCR) in K562/ADM cells was detected using flow cytometry. Intracellular accumulation of ADM was measured by fluorescence spectrophotometry. Flow cytometry was used to investigate the efflux of rhodamine 123 (Rh123) and the expression of P-glycoprotein (P-gp) in K562/ADM cells. RESULTS: Lom increased the cytotoxicity of ADM and the apoptosis induced by ADM or VCR in K562/ADM cells. At the concentration of 3, 10 and 30 micromol x L(-1), Lom reduced the IC50 value of ADM from 79.03 micromol x L(-1) to 28.14, 8.16 and 3.16 micromol x L(-1), respectively. Lom increased the intracellular accumulation of ADM and inhibited the efflux of Rh123 in K562/ ADM cells. No change in P-gp expression was observed after the treatment of Lom for 72 h. CONCLUSION: Lom had strong reversal effect on MDR in K562/ADM cells by inhibiting P-gp function.

ATP Binding Cassette Transporter, Subfamily B, Mem↗
PubMed2003

Studies on antidiabetic effects of cortex Moutan polysaccharide-2b in type 2 diabetes mellitus rats.

AIM: To study the antidiabetic effects of cortex Moutan polysaccharide-2b (CMP-2b) in type 2 diabetes mellitus (T2DM) rats. METHODS: The T2DM model rats were induced by a single intravenous injection of low dose streptozotocin (STZ) and intake of high sucrose-fat diet. CMP-2b was given to T2DM rats daily through gavage for 4-5 weeks. The body weight, water and food intake, fasting blood glucose (FBG), glucose tolerance, plasma lipids, serum insulin, and insulin receptor (Ins R) were determined. RESULTS: Oral administration of CMP-2b significantly decreased water and food intake, FBG, total cholesterol (Tch), and triglyceride (TG), improved the impaired glucose tolerance (IGT), and remarkably raised the number of low affinity InsR and insulin sensitivity index (ISI) in T2DM rats. CONCLUSION: CMP-2b may be useful for treating T2DM and its complications.

Animals↗
PubMed2003

9-(4-ethoxycarbonylphenoxy)-6,7-dimethoxy-1,2,3,4-tetrahydro acridine inhibits free radical induced rat cortical neuron cytotoxicity and cerebral ischemia injury.

AIM: To study the effects of 9-(4-ethoxycarbonylphenoxy)-6,7-dimethoxy-1,2,3,4-tetrahydro acridine (EDT) on free radical induced injury in primary cultured rat cortical neuron and cerebral ischemia in mice. METHODS: In primary rat cortical neuron, free radical injury model was established by 10 mumol.L-1 H2O2. The content of malondiadehyde (MDA) and activity of superoxide dismutase (SOD) in cells were investigated. Chronic cerebral ischemia model was produced by occlusion of one carotid artery and pneumogastric nerve in mice. The step down test was adopted to investigate the effect of EDT on the memory impairment. The cerebra morphology and MDA, NO content and SOD activity in mice cerebra were detected. RESULTS: In primary rat cortical culture, 0.01-3 mumol.L-1 EDT concentration-dependently inhibited the formation of MDA and reduction of SOD activity induced by 10 mumol.L-1 H2O2. In chronic cerebral ischemia, EDT 2.5, 5 and 10 mg.kg-1 ig for 5 d greatly improved the memory impairment, reduced NO efflux and MDA content, while increased SOD activity in mice cerebra. CONCLUSION: EDT was found to protect neurons from H2O2-induced neurotoxicity and inhibit chronic cerebral ischemia mediated injury and memory impairment in mice.

Acridines↗
PubMed2002

[Clinical value of spiral CT in the surgical treatment of severe acute pancreatitis].

OBJECTIVE: To evaluate the clinical value of spiral CT in the surgical treatment of severe acute pancreatitis (SAP). METHODS: The morphological changes in the spiral CT images of 82 SAP patients were contrasted with the therapeutic methods and the curative effects. RESULTS: The operation for obstructive biliogenic pancreatitis, invasive parapancreas vessels forming angioma, piece and segmental necrosis in the body and tail of the pancreas, the enlargement of pancreas cyst during the treatment, and parapancreas abscess diagnosed by the spiral CT images had a significant curative effect compared with the non-operation treatment (P < 0.05). The satisfactory effect could be gained if a conservative treatment was performed first, and then the delayed operative treatment was carried out according to the etiologic and clinical data for the SAP patients with extensive phlegmona, necrosis in the head of the pancreas, severe parapancreas cyst, and a large amount of ascite. CONCLUSION: Spiral CT can supply the grounds for choosing surgical methods and the surgical opportunity in the treatment of SAP patients.

Acute Disease↗