Biomedical subjects

Gustav Amberg

Publications and source records attributed to Gustav Amberg.

3 recordsLinked to original sources

PubMed2006

Multi-step dielectrophoresis for separation of particles.

A new concept for separation of particles based on repetitive dielectrophoretic trapping and release in a flow system is proposed. Calculations using the finite element method have been performed to envision the particle behavior and the separation effectiveness of the proposed method. As a model system, polystyrene beads in deionized water and a micro-flow channel with arrays of interdigited electrodes have been used. Results show that the resolution increases as a direct function of the number of trap-and-release steps, and that a difference in size will have a larger influence on the separation than a difference in other dielectrophoretic properties. About 200 trap-and-release steps would be required to separate particles with a size difference of 0.2%. The enhanced separation power of dielectrophoresis with multiple steps could be of great importance, not only for fractionation of particles with small differences in size, but also for measuring changes in surface conductivity, or for separations based on combinations of difference in size and dielectric properties.

Electrodes↗

PubMed2005

Superpositioned dielectrophoresis for enhanced trapping efficiency.

One of the major applications for dielectrophoresis is selective trapping and fractionation of particles. If the surrounding medium is of low conductivity, the trapping force is high, but if the conductivity increases, the attraction decreases and may even become negative. However, high-conductivity media are essential when working with biological material such as living cells. In this paper, some basic calculations have been performed, and a model has been developed which employs both positive and negative dielectrophoresis in a channel with interdigitated electrodes. The finite element method was utilized to predict the trajectories of Escherichia coli bacteria in the superpositioned electrical fields. It is shown that a drastic improvement of trapping efficiency can be obtained in this way, when a high conductivity medium is employed.

Electric Conductivity↗

PubMed2003

Semisharp phase field method for quantitative phase change simulations.

The standard phase field model for simulation of phase change requires an asymptotic analysis in a vanishing interface width, in order to connect the model parameters to the sharp interface parameters, which has hampered the quantitative usefulness of the method. In this Letter the method is simplified to the point that the relevant reduced problem can be solved analytically, allowing the sharp and phase field parameters to be identified, in principle, without restrictions on the model parameters. The scheme is tested for standard cases of two-dimensional solidification, showing excellent agreement with sharp interface kinetics.

Journal Article↗