Biomedical subjects

György Acsády

Publications and source records attributed to György Acsády.

16 recordsLinked to original sources

PubMed2006

Factor V Leiden and apolipoprotein E genotypes in severe femoropopliteal atherosclerosis with restenosis.

BACKGROUND: The role of factor V (Leiden) mutation, thrombophilia, and apolipoprotein E (apoE) alleles in the pathogenesis of accelerated atherosclerosis and restenosis was studied in patients requiring reoperation within five years after femoropopliteal angioplasty with artificial grafts. METHODS: One hundred ninety-eight consecutive patients with femoropopliteal atherosclerotic disease, reoperated for restenosis were contacted by phone and 100 of them returned for laboratory and clinical work-up. In addition to clinical evaluation and routine laboratory investigations, parameters of lipoprotein metabolism, factor V (Leiden) mutation and apolipoprotein E (apoE) allele were studied by PCR amplification of DNA and endonuclease digestion techniques. RESULTS: A significantly higher incidence of factor V (Leiden) mutation was found in patients with atherosclerosis and restenosis, compared to 445 healthy blood donors (13/200, 6.5% vs. 34/890, 3.8%, p=0.0379). Distribution of the alleles of the apolipoprotein E (apoE) gene was different, when the patients were compared to 372 controls; however, the difference only approached the level of statistical significance (25/200, 12.5% vs. 56/744, 7.5%, p=0.0515). Comparing the two groups, the number of epsilon4 allele carriers was significantly higher among patients with restenosis (25/100, 25% vs. 53/272, 14%, p=0.0147). CONCLUSION: Factor V (Leiden) mutation may influence the progression of atherosclerosis and the development of restenosis after revascularization in patients with accelerated femoropopliteal atherosclerosis. Further investigation is needed whether long-term anticoagulation has an impact or not on the course of disease in such cases. ApoE epsilon4 allele should be screened in patients with femoropopliteal atherosclerosis, because it indicates a faster progression of atherosclerosis and may predict restenosis after revascularization procedure.

Adult↗

PubMed2006

[Risk of cardiac complications in patients undergoing major vascular surgery].

In patients undergoing major vascular surgery cardiac complications are the major cause of perioperative morbidity and mortality. This is related to the frequent presence of underlying coronary artery disease. The aim of the preoperative cardiac risk assessment is to evaluate the presence and degree of coronary artery disease along with other risk factors such as cerebrovascular disease, renal insufficiency, and diabetes mellitus that may influence the perioperative risk of these patients. Based on the presence and number of cardiac risk factors the individual risk profile can be determined, which may facilitate medical decision making about the perioperative and long-term management of these patients. In the present review based on data from current literature we summarized the pathology of perioperative cardiac complications, and the role of cardiac risk assessment and risk reduction strategies in patients undergoing major vascular surgery. In this review the authors summarized data from current literature and found that a limited set of risk factors and a result of a noninvasive test used for detection of coronary artery disease may help to stratify patients into low-, intermediate- and high risk for perioperative cardiac complications. If patients with intermediate- to high-risk prescribed beta-blockers the risk of cardiac complications can be reduced. Along with beta-blockers it was also found that perioperative use of statins may also help to reduce the risk of cardiac complications. In patients identified to be at very high-risk further perioperative evaluation and management should include coronary angiography with subsequent coronary revascularization if there is a clearly defined need, independent of the need for vascular surgery.

Adrenergic beta-Antagonists↗

PubMed2006

Human blood plasma advanced oxidation protein products (AOPP) correlates with fibrinogen levels.

In 1996 a novel oxidative stress biomarker, referred to as advanced oxidation protein products (AOPP) was detected in the plasma of chronic uremic patients. The aim of the present studies was to find out that which plasma fraction(s) is responsible for AOPP reactivity. Thermal treatment of pooled samples of human citrate-plasma or EDTA-plasma at 50 degrees C resulted in a rapid and parallel loss of fibrinogen concentration and AOPP reactivity. On the basis of time course and t1/2 values following thermal treatment, AOPP was indistinguishable from fibrinogen. There was a statistically significant (p < 0.0001) correlation between levels of blood plasma fibrinogen and AOPP in patients (n = 61) with various peripheral vascular or cardiovascular diseases. There was also a significant (p < 0.0001) relationship between plasma levels of fibrinogen and molar AOPP/fibrinogen ratio indicating that higher fibrinogen concentrations were associated with more oxidatively transformed groups on the molecule. Results of the present studies suggest that post-translationally modified fibrinogen is a key molecule responsible for human plasma AOPP reactivity. It remains to be elucidated what is the pathophysiological significance of the post-translationally modified fibrinogen in the inflammation-associated events of atherosclerosis, in platelet aggregation, and as a cardiovascular risk biomarker.

Biomarkers↗

PubMed2006

The effect of induction method on defibrillation threshold and ventricular fibrillation cycle length.

INTRODUCTION: Since no clinical data are available on the comparison of the "shock on T-wave" and "high frequency burst" ventricular fibrillation (VF) induction modes during defibrillation threshold (DFT) testing, we aimed to compare these two methods during implantable cardioverter defibrillator implantation. METHODS: The DFT was determined with a step-down protocol using biphasic, anodal polarity (100%, 40%, 20% voltage control) shocks. Patients were randomized: VF was induced by 50 Hz burst in group B (n = 45) and T-wave shock in group T (n = 41). The DFT was defined as the lowest energy level that terminated VF; confirmed DFT (DFTc) was defined as the minimal energy level that consecutively terminated VF twice. Success rate of DFTc was calculated during an intraindividual test for the alternate induction method. RESULTS: A total of 546 episodes of VF were induced: n = 278 (B) vs n = 268 (T). Incidence of VT during inductions was 9.9% (B) vs 2.7% (T), P < 0.05. Neither the DFT, 8.8 +/- 4.0 J (B) vs 9.7 +/- 4.2 J (T), nor the DFTc, 10.6 +/- 5.1 J (B) vs 10.8 +/- 4.2 J (T), proved to be significantly different. A significant correlation was found between VF cycle length (CL) and the concomitant DFT (r = 0.298, P < 0.05) in group T only. Subgroup analysis of patients under chronic class III antiarrhythmic treatment showed no increase of the DFT in either group and significantly lower incidence of VT induction in group T regardless of antiarrhythmic treatment. CONCLUSION: The DFT and the VFCL proved to be independent of the VF induction method. The T-wave shock was more unlikely to induce VT during DFT testing. These results suggest that both methods are reliable in DFT determination, though T-wave shock application is a more reliable method for DFT testing.

Aged↗

PubMed2006

Elevated complement C3 is associated with early restenosis after eversion carotid endarterectomy.

Early restenosis following carotid endarterectomy (CEA) is an inflammatory process leading to myointimal hyperplasia of smooth muscle cells. The risk for restenosis is increased in homozygous carriers of the normal (A) allele of mannose-binding lectin (MBL2) gene. Our objective was to study the associations of C3 and as control three non-complement acute-phase reactants (APRs) (C-reactive protein, haptoglobin and alpha2HSglycoprotein) with early restenosis following CEA. We also considered, whether MBL2 genotype relates to C3 levels and to the risk of restenosis. Concentrations of the APRs were determined by radial immunodiffusion or immunoturbidimetric methods in 64 patients who underwent eversion CEA and were followed up with carotid duplex scan (CDS) examinations for at least one year. MBL2 genotypes were determined by a PCR-SSP method. C3 levels increased during the follow-up and correlated with the percentage of restenosis detected by CDS at 14 months postsurgery, in MBL2 A/A allele carriers. Patients with high C3 levels had nearly five-fold higher odds for the presence of significant restenosis (>50% reduction in diameter) even after adjusting for MBL2 genotype, age and gender. By contrast, no such associations were detected between the non-complement APRs and early restenosis. C3 is associated with and might have a direct role in the development of an early restenosis following CEA, which is partially related to an intact MBL lectin pathway, thus determining C3 levels might have clinical importance. On the other hand, our results indicate that the regulation of C3 differs from non-complement APRs.

Adult↗

PubMed2005

Generalized changes in venous distensibility in postthrombotic patients.

INTRODUCTION: In situ biomechanical properties of peripheral large veins were compared between asymptomatic young patients who had previously unilateral femoro-popliteal deep venous thrombosis (DVT) and age-matched, healthy controls; the aim of this study was to assess local or generalized alterations of venous wall biomechanics in postthrombotic patients. PATIENTS AND METHODS: Inner diameters of both common femoral veins, right axillary vein, and right internal jugular veins were measured in two directions by ultrasonography. Venous pressure was altered by posture changes (standing and lying) and by application of graded and controlled Valsalva. Ten postthrombotic young patients without any symptoms and 11 age-matched control subjects were included. RESULTS: In postthrombotic patients, both the affected and unaffected common femoral vein diameters and capacities were larger at low transmural pressures than those for the control group, but they demonstrated significantly less distensibility when higher pressures were applied. Similarly, in the internal jugular vein, capacity without Valsalva was significantly higher in postthrombotic patients and distensibility was reduced (statistically significant in the erect position). Pressure-induced changes in axillary vein diameter were negligible. CONCLUSIONS: In situ diameter and capacity changes, and in situ distensibility of the femoral veins on both sides (i.e., the side of previous thrombosis as well as the disease-free side) and of the jugular veins are reduced in the young DVT patients compared to veins of the age-matched, healthy controls. The pathophysiological mechanism of generalized venous wall changes in these young DVT patients remains unknown.

Adolescent↗

PubMed2005

Dramatic changes in the serum levels of anti-cholesterol antibodies after eversion endarterectomy in patients with severe carotid atherosclerosis.

Our goal was to study changes in anti-cholesterol antibodies (ACHA) levels in patients with severe carotid stenosis after eversion endarterectomy. Seventy consecutive patients who underwent eversion endarterectomy at the Department of Cardiovascular Surgery, Semmelweis University, Budapest, were included in the study. Serum samples from 66 healthy volunteers served as controls. Patients had medical check up at 5.7 (4.6-8.0) weeks (median (interquartile range)), 6.8 (6.2-7.9) months as well as 13.8 (12.3-19.0) months after endarterectomy. In all patients the carotid arteries were investigated by color duplex ultrasound. ACHA concentrations were determined in the serum samples taken before operation as well as at the first and last follow-up visits by using an ELISA method. ACHA concentrations (median (interquartile range) were found to be significantly (p<0.0001) lower in the sera of the patients with carotid atherosclerosis (13.5 (8.4-21.3)AU/ml) than in the healthy subjects (26.1 (20.9-33.2)AU/ml) (Mann-Whitney test). Strong negative correlation was found between the preoperative ACHA and LDL-cholesterol levels (r=-0.413, p=0.0004). Serum ACHA concentrations significantly (p<0.0001) increased from the values measured before operation (13.5 (8.4-21.3)AU/ml) to 27.1 (19.9-34.7)AU/ml measured at the end of the 14 months long follow-up. Increase occurred only in patients with low or medium baseline ACHA concentration. Our present findings indicate that after surgical removal of atherosclerotic plaques from the carotid arteries the reduced baseline levels of ACHA reach normal values in 1 year. Different not mutually exclusive mechanisms (binding of ACHA to lipid/lipoproteins particles, to advanced plaques or reversibly injured endothelial cells in CNS) can be responsible for this novel finding.

Acute-Phase Proteins↗

PubMed2005

[Case of a young woman successfully operated on for acute pulmonary embolism and right ventricular thrombus after urogenital infection].

The authors report a case of a 22-year-old women operated on for pulmonary embolism with success. The venous embolism developed in two phases one week apart. The first clinical sign was a dyspnea of abrupt onset limiting physical activity even after a mild exertion. However, relevant diagnostic tests were performed after one week when the patient fainted, her dyspnea worsened. The results of ECG, echocardiography and pulmonary perfusion scintigraphy indicated the presence of a thrombus residing in the right ventricle and the embolism of the right pulmonary artery. Chest CT and pneumo-angiography findings show the embolus completely blocking the main right branch of the pulmonary artery and partially blocking the left lower lobe artery. On the basis of their experiences published earlier the patient was brought to a stable cardio-respiratory state prior to surgery. Both the adherent right ventricular thrombus and the fresh embolus residing in the right branch of the pulmonary artery were removed under the protection of extracorporeal circulation. After the elapse of an event-free 6-month postoperative period the patient continued to remain symptomless, while being on a long-term anticoagulant regimen. The long-term anticoncipient medication and the urogenital infection 3 months prior to surgery may have been involved in the etiology of the pulmonary embolism. The authors propose the implementation of an antithrombotic prophylaxis in young women after gynecological infections.

Acute Disease↗

PubMed2005

Advanced oxidation protein products (AOPP) for monitoring oxidative stress in critically ill patients: a simple, fast and inexpensive automated technique.

Oxidative stress is known to be involved in many human pathological processes. Although there are numerous methods available for the assessment of oxidative stress, most of them are still not easily applicable in a routine clinical laboratory due to the complex methodology and/or lack of automation. In research into human oxidative stress, the simplification and automation of techniques represent a key issue from a laboratory point of view at present. In 1996 a novel oxidative stress biomarker, referred to as advanced oxidation protein products (AOPP), was detected in the plasma of chronic uremic patients. Here we describe in detail an automated version of the originally published microplate-based technique that we adapted for a Cobas Mira Plus clinical chemistry analyzer. AOPP reference values were measured in plasma samples from 266 apparently healthy volunteers (university students; 81 male and 185 female subjects) with a mean age of 21.3 years (range 18-33). Over a period of 18 months we determined AOPP concentrations in more than 300 patients in our department. Our experiences appear to demonstrate that this technique is especially suitable for monitoring oxidative stress in critically ill patients (sepsis, reperfusion injury, heart failure) even at daily intervals, since AOPP exhibited rapid responses in both directions. We believe that the well-established relationship between AOPP response and induced damage makes this simple, fast and inexpensive automated technique applicable in daily routine laboratory practice for assessing and monitoring oxidative stress in critically ill or other patients.

Adolescent↗

PubMed2005

[Congenital arteriovenous malformation complicated with brachial artery false aneurysm].

Congenital arteriovenous malformations in the upper extremity, mainly in the hand are rare. In this patient an arteriovenous fistula of the hand caused arterial dilatation of the upper limb, and blunt injury of brachial artery resulted in an extreme large false aneurysm. This situation, which threatened with rupture and peripheral embolisation was treated by resection of the false aneurysm with end-to-end direct anastomosis. Complication of a congenital arteriovenous malformation so late, in the ninth decade is almost exceptional. We think that the successful surgical treatment of an infrequent lesion's rare late complication is worth presenting.

Aged, 80 and over↗

PubMed2004

[New chapter of vascular surgery; stent-graft placement in the treatment of aortic aneurysm].

INTRODUCTION AND AIM: This paper deals with a novel chapter of aneurysm surgery, the stent-graft implantation. The interdisciplinary cooperation between angio-radiology and surgery made available a much less invasive therapeutic option. The new technique was first applied in 1991.The authors present the technique, conditions and results of the procedure and also give a survey on the domestic situation. METHOD: In general anaesthesia using inguinal approach a self-expanding stent-graft (diameter: 8 mm) equipped with barbs was inserted in the aorta and deployed into the aneurysm under X-ray guidance. Vanguard (Boston-Scientific Co.), Talent (Medtronic Co.) and Exluder (Gore-Tex Co.) instruments were used. RESULTS: In 40 patients (29 men and 11 women, mean age: 59.7 years) 55 stent-grafts were implanted. Thirty-one thoracic and 9 infrarenal aneurysms were treated. In 3 patients ruptured aneurysm, in 3 other instances covered ruptured aneurysm, and in 3 persons symptomatic aortic aneurysm were treated. Thirty-one patients underwent elective procedures. Two deaths occurred in the early, and 4 in the late postoperative period (at months 3, 6, 7 and 36 postoperatively). In 9 patients adjunctive vascular surgery interventions were performed in 13 instances. Primary endoleak occurred in 3, whereas secondary endoleak was observed in 4 instances. All of these healed spontaneously. The authors compared the domestic results with internationally published data. This included 1120 traditional aneurysm resections and 110 stent-graft implantations performed in Hungary and 2283 surgical and 3843 endovascular procedures published in international scientific journals. CONCLUSIONS: The indications for this new procedure are still being formed. Despite the higher incidence of complications, this less traumatic intervention can be applied with benefit for patients. Nevertheless, at the time being, financial considerations may prevent the more widespread use in the practice. In Hungary the conditions are already available for this endovascular treatment and the authors support its employment.

Adolescent↗

PubMed2004

[Treatment of acute ST-elevation myocardial infarction with primary percutaneous coronary intervention].

INTRODUCTION: Treatment of acute myocardial infarction has changed significantly in recent years. Since January 1st, 2003 primary coronary intervention has been organized in Budapest. The authors show the results during the implementation of this method in the first 6 months with emphasizing its importance. PATIENTS: In the center which was developed specially for catheter interventions in myocardial infarction, 282 patients were admitted during this period with the suspicion of acute ST-elevation myocardial infarction. 266 (94.3%) of them were proved to have an infarction. Of these, 260 had a lesion amenable for percutaneous intervention. RESULTS: Success rate of catheter-based revascularization was 96.5%. 4 patients underwent open-heart surgery. Adverse events were: severe bleeding 1.1%, in-hospital mortality 4.5%. CONCLUSION: In our center the major outcomes are similar to the results of large international centers.

Adult↗

PubMed2004

Parasympathetic cardiac nerve stimulation with implanted coronary sinus lead.

A patient with drug-refractory paroxysmal atrial fibrillation associated with rapid ventricular rate underwent biatrial pacemaker implantation. During elective replacement of the pacemaker, a significant voltage- and frequency-dependent decrease in ventricular rate was achieved by high-frequency electrical stimulation (17 Hz) of parasympathetic cardiac nerves innervating the AV node with the implanted bipolar coronary sinus electrode. The negative dromotropic effect of parasympathetic stimulation was eliminated by intravenous administration of 1-mg atropine.

Anti-Arrhythmia Agents↗

PubMed2004

Magnetic resonance imaging-based biventricular pacemaker upgrade.

This report describes a patient with drug refractory severe chronic ischemic heart failure, atrial fibrillation with bradycardia, and left bundle branch block who had a failed implantation of a biventricular pacemaker because of a high left ventricular pacing threshold. VVI pacemaker implantation had not improved the patient's condition. MRI-guided biventricular pacemaker upgrade had been performed with a left ventricular epicardial lead at the lateral region where a 4-mm thickening during systole had been proven. After 6 months of effective resynchronization, the patient's functional class improved to NYHA II without further need of hospitalization.

Atrial Fibrillation↗

PubMed2004

[The advantages of axillary artery cannulation in surgical treatment of type A acute aortic dissection].

There are more alternative cannulation techniques during surgery of type A aortic dissection. The most frequently used femoro-atrial cannulation method provides limited possibility for brain protection during surgery. This theory is confirmed by relatively high frequency of major brain complications in patients operated on while using this cannulation technique. During the last years cannulationis used more often, as it may provide more protection for the brain than other methods. In 2003 seven patients underwent aortic reconstruction because of type A acute aortic dissection using axillary cannulation. All patients except one were discharged after uneventful recovery. There were no postoperative neurological complications following surgery. We lost one patient due to distal progression of the dissection. He was the only patient with clinical evidence of transient postoperative brain damage. We are strongly convinced that the spectacular improvement in our results for the surgery of type A acute aortic dissection is due to the axillary cannulation, the anterograde flow and the isolated cerebral perfusion. We recommend the axillary cannulation technique as the first choice in type A acute aortic dissection.

Acute Disease↗

PubMed2002

[Secondary aorto-enteral fistula--material of 22 years and literature review].

INTRODUCTION: Aorto-enteral fistula is a pathological communication between the aorta and the bowel lumen. In its secondary form is a dreaded, often fatal complication of the aorto-iliac operations. METHODS: We report 34 secondary fistulas, a great number of patients treated in the last 22 years. One can rarely find such a number in the literature. RESULTS: The incidence of secondary fistulas is 0.42% following aorto-iliac operations. We give a detailed description of diagnostics. We prefer in situ solutions (graft change, aorto-aortic interposition, iliac desobliteration, homograft or deep venous bypass, aorto-bifemoral bypass) to extra-anatomical bypasses. We emphasize the importance of the postoperative care, and the necessity of adequate antibiotic therapy. CONCLUSION: Despite improved operations and intensive care mortality is still high (55.88%). We find that prevention is of great importance.

Aorta, Abdominal↗