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H A Andersen

Publications and source records attributed to H A Andersen.

At least 19 recordsLinked to original sources
PubMed1992

A peptide from Tetrahymena disrupts subunit organization of E. coli RNA polymerase.

Incubation of the E. coli RNA polymerase with a polypeptide factor from the protozoan Tetrahymena reduces the affinity of the holoenzyme for DNA. SDS-polyacrylamide gel electrophoresis of the peptide-treated RNA polymerase showed that the band pattern of the polymerase subunits was strongly altered. The three large subunits, beta', beta and sigma, disappear and a high number of rapidly migrating bands appeared. However, a brief heat treatment of the samples almost restored the original RNA polymerase subunit composition, and in addition a high molecular weight protein band approximately 240 kDa appeared. It is suggested that the Tetrahymena peptide specifically binds to the RNA polymerase and changes the structures of the large subunits.

Animals↗
PubMed1989

Purification and partial characterization of a transcription-inhibitory peptide from Tetrahymena.

The protozoa Tetrahymena excretes a small peptide complex with an Mr of about 5,000. The peptide inhibits transcription by reducing the activity of the RNA polymerase. We have purified and partially characterized the peptide complex. It contains two peptide chains of apparent Mr 2,300 and 2,600, respectively. Magnesium ions in connection with the SH groups of cysteine play a role in holding together the two chains of the intact complex.

Amino Acids↗
PubMed1987

Peptides regulate the activity of RNA polymerases in Tetrahymena.

An exponentially multiplying population of the protozoa Tetrahymena has a highly variable transcription rate when cultivated in a complex broth medium. It is shown that peptides in the medium specifically stimulate transcription and that the cells in response synthesize a peptide with a molecular weight of 3000-4000. This peptide inhibits transcription in vivo in cells with high rates of transcription and in in vitro transcription systems. We have partially purified radioactively labelled inhibitor peptide and found that cells stimulated to high transcription rate selectively accumulate inhibitor peptide in the nuclei. In vitro experiments have shown that the inhibitor peptide can reduce the RNA polymerase activity in the nuclei. The presence of inhibitor seems to lower the affinity of RNA-polymerases towards DNA and to increase their release during incubation of isolated nuclei. On the other hand, stimulatory peptides will compete with the inhibitor, and the polymerase activity in isolated nuclei is determined--at least partially--by the ratio between stimulatory and inhibitory peptide factors. It is suggested that regulatory peptides also are involved in regulation of transcription in vivo.

Animals↗
PubMed1979

Regulation of RNA synthesis in Tetrahymena pyriformis: secretion of regulatory factors.

The rate of ribosomal RNA synthesis varies greatly with the population density in both exponentially and synchronously growing populations of Tetrahymena pyriformis. Shortly after inoculation of the population - at relatively low cell densities - a gene-dose effect dominates the picture, and a doubling in the gene number is immediately followed by a doubling in the rate of RNA synthesis. However, also other mechanisms are controlling the rate of RNA synthesis. Generally one finds high rates of RNA synthesis in the lag phase of newly inoculated cells, decreasing rate of RNA synthesis during most of the exponential growth phase and very low rate of synthesis in stationary phase cells. We now have results which show that the repression of RNA synthesis in densely populated cultures is caused by a dialysable factor, which is secreted by the cells. If cells are inoculated on a medium which contains this factor the high initial rate of RNA synthesis normally observed is prevented, but the cells multiply and grow with normal generation time until normal stationary-phase population densities are reached.

Animals↗
PubMed1978

Regulation of ribosomal RNA synthesis in Tetrahymena pyriformis.

Ribosomal RNA is synthesized at constant rate during most of the cell cycle in heat-shock synchronized populations of Tetrahymena pyriformis. Early in each macronuclear S-period the rate of synthesis increases abruptly, concomitant with replication of the genes coding for ribosomal RNA. The increase is prevented by inhibitors of DNA replication, added prior to the S-period. Similarly, in cultures synchronized by starvation/refeeding, inhibition of DNA replication, at the time when the rDNA is replicated, will prevent the normal increase in rate of RNA synthesis which follows refeeding. We conclude that inhibition of rDNA replication interferes with the synthesis of rRNA, and we suggest that with respect to rRNA synthesis a gene dosis effect is operating in fast-growing Tetrahymena cells.

Animals↗
PubMed1977

Preferential inhibition of rDNA transcription by 5-bromodeoxyuridine.

On a chemically defined growth medium the degree of substitution of thymidine with 5-bromodeoxyuridine (BUdR) in DNA of Tetrahymena pyriformis was controlled by the concentration of tetrahydrofiolic acid, BUdR and thymidine in the medium. A correlation between the degree of BUdR substitution in DNA and the reduction in rate of total RNA synthesis has been established. It was found that the reduction of total RNA synthesis results from inhibition of transcription of all RNA species which have been measured. However, independent of the degree of BUdR substitution in DNA, a preferential inhibition of the synthesis of 25s and 17s ribosomal RNA was found. It is concluded that the various genes may respond differently to BUdR substitution with respect to transcription.

Bromodeoxyuridine↗
PubMed1975

Letter: Sarcoidosis.

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Adrenal Cortex Hormones↗
PubMed1975

Letter: Sarcoidosis.

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Adrenal Cortex Hormones↗