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H A Gaafar

Publications and source records attributed to H A Gaafar.

47 records · Page 3Linked to original sources
PubMedDate not supplied

Modified immunofluorescence test for the serologic diagnosis of gonorrhea (FGT-ABS).

Investigation of the source of false-positive reactions in the Fluorescent Gonococcal Test-Heated (FGT-H) implicated antibodies to antigens common to Neisseria gonorrhoeae and N. meningitidis. Absorption experiments proved that the fluorescence was due to both species-specific and common antigens and that in most cases the common antibodies could be successfully absorbed by a soluble protein extract of N. meningitidis. The value of such an absorption step was examined using sera from 500 women, 73 from bacteriologically confirmed cases of gonorrhea and 427 from bacteriologically and clinically negative cases. The absorption step reduced the false positivity rate by 58% without a significant change in sensitivity. The modified procedure, the Fluorescent Gonococcal Antibody Test-Absorbed (FGT-ABS), has a sensitivity similar to the Fluorescent Gonococcal Test-Heated (FGT-H) but has the advantage of higher specificity.

Antibodies, Bacterial↗
PubMedDate not supplied

Screening for Trichomonas vaginalis infection by use of acridine orange fluorescent microscopy.

The acridine orange test for detection of Trichomonas vaginalis in smears has been adapted for delayed examination of specimens. Mailed-in slides stained by acridine orange were compared with on-site wet mounts; the acridine orange test detected 96% of all positives, whereas only 76% were detected by wet mounts. In a similar comparison with Papanicolaou smears, the acridine orange test detected 89% as compared with 67% detected by Papanicolaou smears.

Acridine Orange↗
PubMedDate not supplied

Skin affection in rhinoscleroma. A clinical, histological and electron microscopic study on four patients.

Clinical, histological and electron microscopic studies were performed on 4 rhinoscleroma patients with concomitant skin lesions in the upper lip, dorsum of the nose and nasolacrymal sac area. The skin lesions were treated locally. One of the patients was followed up to 16 years. Histologically, the skin lesions showed downward prolongation of the rete pegs. Deep in the dermis, vacuolated Mikulicz cells surrounded by lymphocytes and plasma cells were found. By electron microscopy, numerous small vacuoles containing fine granular material were seen inside Mikulicz cells. A limited number of bacilli were found inside and outside these cells. Two clinical entities of rhinoscleroma are observed in Egypt, an active granulomatous type with possible extranasal extension, and a less active intranasal type with limited mucosal lesions. The skin lesions in rhinoscleroma were found to have an unpredictable course.

Adolescent↗