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Biomedical subjects

H A Hadi

Publications and source records attributed to H A Hadi.

At least 19 recordsLinked to original sources
PubMed2001

Identification and characterization of App: an immunogenic autotransporter protein of Neisseria meningitidis.

In a search for immunogenic virulence factors in Neisseria meningitidis, we have identified a gene encoding a predicted 160 kDa protein with homology to the autotransporter family of proteins. Members of this family are secreted or surface exposed and are often associated with virulence in Gram-negative bacterial pathogens. We named the gene adhesion and penetration protein (app), because of its extensive homology to the hap gene of Haemophilus influenzae. We reconstructed the gene with reference to genomic sequence data and cloned and expressed the protein in Escherichia coli. Rabbit antiserum raised against recombinant App reacted with proteins in all meningococcal isolates examined, which represented clonal groups responsible for the majority of meningococcal invasive disease. Antibodies to the protein were detected in the sera of patients convalescing from meningococcal infection. Purified App had strong stimulating activity for T cells isolated from a number of healthy donors and from one convalescent patient. We confirmed that App is surface localized, cleaved and secreted by N. meningitidis. Importantly, the rabbit anti-App serum killed the organism in the presence of complement. Thus, App is conserved among meningococci, immunogenic in humans and potentially involved in virulence. It therefore merits further investigation as a component of a future multivalent vaccine.

Antigens, Bacterial↗
PubMed2000

Structure-activity relationship of polyisoprenyl benzophenones from Garcinia pyrifera on the tubulin/microtubule system.

Microtubule disassembly inhibitory properties have been established for the known polyisoprenylated benzophenones xanthochymol (1a) and guttiferone E (1b). The compounds were isolated from the fruits of Garcinia pyrifera collected in Malaysia. A structure-activity relationship study, including natural and semisynthetic derivatives, delineated some structural features necessary for the interaction with tubulin within this compound class.

Benzophenones↗
PubMed1999

Cytotoxic polyisoprenes and glycosides of long-chain fatty alcohols from Dimocarpus fumatus.

The ethanolic extract from the stem bark of Dimocarpus fumatus, showed in vitro cytotoxic activity against KB cells. Fractionation of the extract gave compounds belonging to different classes. The two major components have been identified as a benzoquinone, sargaquinone, and a chromene, sargaol. One sphingolipid, soyacerebroside I, two glycosides of sitosterol, and fatty acids were also identified. Besides these known compounds, two new glycosides of long-chain fatty alcohols have been identified as 1-O-[alpha-L-rhamnopyranosyl-(1-->2)-beta-D- glucopyranosyl-(1-->3)-alpha-L-rhamnopyranosyl-(1-->6)-beta-D- glucopyranosyl]hexadecanol and 1-O-[[alpha-L-arabinopyranosyl-(1-->3)]-alpha-L-rhamnopyranosyl-(1 -->2)- beta-D-glucopyranosyl-(1-->3)-alpha-L-rhamnopyranosyl-(1-->6)-beta-D- glucopyranosyl] hexadecanol, and a mixture of three new diacylglycerylglucosides has been isolated. These structures were elucidated by analysis of 2D-NMR and mass spectra.

Animals↗
PubMed1995

Prenatal diagnosis and management of fetal goiter caused by maternal Grave's disease.

We present a case of maternal Grave's disease associated with fetal goitrous hyperthyroidism. Fetal goiter was diagnosed by ultrasound and diagnosis of fetal hyperthyroidism was established by umbilical blood sampling. Fetus was successfully treated by increasing maternal propylthiouracil dosage. Fetal thyroid status was normal at birth. Role of sonography and umbilical blood sampling in management of fetal goiter complicated with maternal Grave's disease is discussed.

Adult↗
PubMed1995

In utero treatment of fetal goitrous hypothyroidism caused by maternal Graves' disease.

We present two cases of maternal Graves' disease complicated by fetal goitrous hypothyroidism. Both patients had elevated maternal thyroid-stimulating immunoglobulin and thyrotropic-binding inhibitory immunoglobulin antibody titers. Diagnosis of fetal hypothyroidism was made by cordocentesis, and serial injections of thyroxine into the amniotic fluid resulted in return to normal of fetal thyroid size and function at birth. Significance of in utero management of fetal hypothyroidism is discussed.

Adult↗
PubMed1995

Clinical significance of neurofibromatosis in pregnancy.

Neurofibromatosis (NF) is a common genetic disease that affects multiple organ systems. We studied eight women with NF and the outcome of their 14 pregnancies. One patient and her fetus died of a massive intracranial hemorrhage as a result of ruptured glioblastoma of the basal ganglia. The incidence of live birth and preterm labor were 50% and 28.6%, respectively. One of 14 pregnancies (7.1%) resulted in spontaneous abortion. There was also one growth-retarded fetus in this series (7.1%). Perinatal implications of this disease and management guidelines are discussed.

Adult↗
PubMed1994

Premature rupture of the membranes between 20 and 25 weeks' gestation: role of amniotic fluid volume in perinatal outcome.

OBJECTIVE: Our purpose was to prospectively study the relationship between amniotic fluid volume and perinatal outcome in pregnancies complicated by premature rupture of the membranes before fetal viability. STUDY DESIGN: The study population consisted of 178 singleton pregnancies with premature rupture of membranes between 20 and 25 weeks' gestation who were managed expectantly. Serial amniotic fluid volume measurements were made and their relationship to the neonatal survival rate, incidence of chorioamnionitis, and other perinatal outcomes was determined. RESULTS: Seventy-four patients were delivered before 25 weeks of gestation and only five infants (6.7%) survived. In contrast, 104 patients were delivered between 26 and 34 weeks, and 93 infants (89.4%) survived (p < 0.001). There were 107 pregnancies with adequate amniotic fluid volume after premature rupture of membranes on admission. Of these 16 patients were delivered before 25 weeks of gestation, and the remaining 91 patients were able to carry their pregnancies beyond 25 weeks of gestation. This was significantly different from 71 patients who demonstrated inadequate amniotic fluid volume on admission to the hospital, of whom 58 were delivered before 25 weeks and only 13 continued the pregnancy beyond 25 weeks (p < 0.05). At gestations between 26 and 34 weeks chorioamnionitis occurred in 22 of 91 (24.1%) patients with adequate amniotic fluid volume versus nine of 13 patients (69.2%) with inadequate amniotic fluid volume (p < 0.001). The incidence of perinatal death for pregnancies between 26 and 34 weeks with adequate versus inadequate amniotic fluid volume was 2.1% and 69.2%, respectively (p > 0.001). Overall survival rate and incidence of chorioamnionitis were 55% and 26.4%, respectively. CONCLUSIONS: Delivery of pregnancies between 20 and 25 weeks of gestation with premature rupture of membranes carries very high risk of neonatal mortality. The results of this study suggest that women with adequate amniotic fluid volume have a better chance to continue their pregnancy beyond 25 weeks of gestation and have a higher neonatal survival rate than those with inadequate amniotic fluid volume. The incidence of perinatal death and chorioamnionitis in patients who carry a pregnancy beyond 25 weeks is correlated with inadequate amniotic fluid volume.

Adult↗
PubMed1994

Prenatal diagnosis of cerebellar hemorrhage: medicolegal implications.

Perinatal brain damage and compromised long-term neurologic outcome have been a topic of major concern and continuing debate. Until recently the occurrence of fetal brain injury was attributed to intrapartum events. However, evidence continues to accumulate that neurologic damage can occur during fetal life unrelated to intrapartum events. We report a case of massive cerebellar hemorrhage of unknown cause diagnosed by ultrasonography at 36 weeks of gestation after decreased fetal movements. Fetal heart rate pattern and Doppler flow study of cerebral arteries are correlated with neuropathologic findings, and the medicolegal aspects of this condition are discussed.

Adult↗
PubMed1994

Electroconvulsive therapy in a twin pregnancy: a case report.

We present a case of twin gestation complicated by severe depression and psychotic behavior; the mother was treated with electroconvulsive therapy (ECT). She had received multiple medications for treatment of her depression earlier during the first part of the pregnancy. However, frequent use of ECT later in the course of pregnancy did not result in adverse fetal outcome as is evident from normal fetal surveillance tests. We conclude that, when indicated, ECT during pregnancy improves maternal condition and does not adversely affect fetal well-being.

Adult↗
PubMed1993

Placental chorioangioma: prenatal diagnosis and clinical significance.

We report the prenatal diagnosis of placental chorioangioma in a 32-week intrauterine pregnancy associated with polyhydramnios and enlarged fetal cardiac size that resulted in intrauterine fetal death. Ultrasound appearance, pathophysiology, and clinical significance of this entity are discussed.

Adolescent↗
PubMed1993

Measurement of pulmonary capillary pressure during ritodrine tocolysis in twin pregnancies: a new noninvasive technique.

Five twin pregnant women were studied during treatment of preterm labor with ritodrine. Maternal pulmonary capillary pressure (PCP) and cardiac function were measured by noninvasive simultaneous recordings of the indirect carotid pulse, electrocardiography, phonocardiography, and M-mode echocardiography. Pulmonary capillary pressure significantly increased from the control value of 9 +/- 3.0 to 18 +/- 2.2 mmHg during the infusion period of therapy. Cardiac index and preejection period to left ventricular ejection time ratio were also increased during treatment with ritodrine. Our findings suggest that noninvasive measurement of PCP and cardiac function may be useful in monitoring twin pregnancies that develop cardiovascular side effects during tocolytic therapy.

Adult↗