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Biomedical subjects

H A Koch

Publications and source records attributed to H A Koch.

At least 19 recordsLinked to original sources
PubMed1989

New method for quantitating yeasts in clinical specimens.

Existing methods for quantitating yeasts in clinical specimens usually rely on plate counts of sediments. A new method has been developed which uses filtration to concentrate a sample and fluorescence microscopy to facilitate counting. This method is fast and easy to perform and can be used for a wide variety of specimens including urine, mouthwash water, and vaginal fluid.

Colony Count, Microbial↗
PubMed1986

Fluorescence microscopy procedure for quantitation of yeasts in beverages.

Existing methods for quantitating yeasts in beverages include time-consuming plate counts that detect only living cells and hemacytometer counts that are reliable only at very high concentrations (e.g., 10(6) to 20 X 10(6) cells per ml). The new method described here involves the use of fluorescence microscopy with the fluorescent stain aniline blue to differentiate yeasts (and other fungi) from backgrounds for easy counting and also may be used in conjunction with membrane filtration to concentrate yeasts from liquids before cell enumeration. Recoveries averaged 91.5% for beverages spiked with levels of 500 to 600,000 organisms per ml. The correlation coefficient of count to spike level was 0.996.

Beverages↗
PubMed1981

[Detection of an alga of the genus Prototheca in the blood of a tuberculosis patient].

The proof of a chlorophyllous alga of the genus Prototheca from the blood of a tuberculous patient with brain damage is described. The pathogenetic importance of Protothecae in the field of human medicine is discussed. Although representatives of the genus Protothecae up to now are regarded as extraordinarily infrequent commensals, nevertheless occasionally their proof not only on the surface of the skin but also from the blood or the inner organs must be taken into account.

Adult↗