PubMed Health⌕ Search

Biomedical subjects

H A Müller

Publications and source records attributed to H A Müller.

At least 91 records · Page 5Linked to original sources
PubMed1981

[Stepwise reconstruction in mid-hand destruction by crush].

The treatment of very serious crush injuries of the metacarpus is reported in three examples. It is recommended that in such injuries reconstruction of all damaged structures should not be performed in the first operation. The most important step beside the stabilisation of the skeleton is microsurgical revascularisation.. The reconstruction of nerves and tendons should be limited during the primary operation. Secondary reconstruction then has a lower risk and gives a good functional result. In such trauma the use of distant flaps for the reconstruction of large soft tissue defects seems more advantageous than the transplantation of free flaps by microvascular anastomoses.

Adolescent↗
PubMed1980

The value of perineal punch biopsy in estimating the histological grade of carcinoma of the prostate.

The degree of accuracy of the histological grade of carcinoma of the prostate obtained by perineal punch biopsy was analyzed by comparing the histological findings with those of radical prostatectomy specimens. Biopsy specimens of 97 of 100 patients who underwent radical prostatectomy between 1969 and 1979 were reevaluated. In 43% of the cases, perineal punch biopsy revealed a uniform architectural pattern, which was classified as well-differentiated adenocarcinoma in 21 cases and poorly differentiated adenocarcinoma in 22 cases. However, of the 21 cases determined by biopsy to be uniformly well-differentiated adenocarcinomas, only seven (33.3%) were confirmed by evaluation of the radical prostatectomy specimens. A uniform, poorly differentiated adenocarcinoma was ultimately found in only five operative specimens of the 22 patients with this preoperative diagnosis. As one case clearly demonstrated, multifocal origin of carcinoma of the prostate is the main source of the frequently occurring error encountered with perineal punch biopsy in determining the grade of differentiation.

Adenocarcinoma↗
PubMed1980

On the development of metastasizing tumors of the rat thyroid gland after combined administration of nitrosomethylurea and methylthiouracil.

Seventy-five female Wistar rats of 150 g each were given 3 X 40 mg/kg body weight nitrosomethylurea (NMU) and 4 weeks later until death (in the 9th-60th week) methylthiouracil (MTU), as a 0.1% solution in their drinking water. After 2 months, in the almost colloid-less thyroid gland individual foci appeared which consisted of sometimes colloid containing follicles with increased cytoplasmic and nuclear basophilia; they increased in number during the subsequent weeks and finally resulted in nodes with different morphologic structures. From the 16th week on there were tumors infiltrating the capsule, and after the 24th week there were angioinvasive carcinomas. Thirteen rats had developed lung metastasization after the 30th week. Cytology could only distinguish between the follicular carcinomas of low differentiation and the follicular adenomas. If these findings are applied to human pathology, one would have to reckon with adenomas which can develop their malignant characteristics without necessarily changing their histologic and cytologic appearance.

Animals↗
PubMed1979

Haemofiltration - critical evaluation of clinical benefits.

To delineate the worth of chronic HF in end stage renal failure, since 1976 we have treated 9 patients with dialysis-resistant hypertension, 6 patients with dialysis intolerance, 7 patients with hypertriglyceridaemia and 7 patients with polyneuropathy. We found an improvement of polyneuropathy and volume-sodium dependent hypertension and symptoms of dialysis discomfort markedly diminished. No amelioration was detected in anaemia, hypertriglyceridaemia and volume-independent hypertension. Hyperphosphataemia was poorly controlled despite increased amounts of aluminium hydroxide. PTH values increased and renal osteopathy seemed to deteriorate.

Acidosis↗
PubMed1978

[The value of fine-needle-cytology, imprint-cytology and histology in the head-neck-region (author's transl)].

Comparative studies about the fine-needle-cytology, imprint-cytology and histology furnished an almost complete agreement between imprint-cytology and histology. Suspected tumor findings by way of fine-needle-biopsy deserve the same place as similar histological examinations. Negative cytological results have to be critically evaluated. Fine-needle-cytology presents a quick and not too painful procedure that is both relatively free of complications and repeatable examination.

Cytological Techniques↗
PubMed1977

[Thin-layer chromatography of sugars (author's transl)].

A thin-layer chromatographic method for the detection of sugars is described. The method can also be adapted for the use in biochemistry, pharmacology, and food chemistry. The solvent systems are: I, benzene/1-butanol/acetone/water (20 : 30 : 40 : 10, v/v); II, 1,2-dichloroethane/glacial acetic acid/methanol/water (50 : 25 : 15 : 10, v/v); III, boric acid 0.5%/1-butanol/2-propanol (20 : 30 : 50, v/v). Detection is developed by a diphenylamine aniline phosphoric acid reagent. Additionally an enzymatic method for the detection of some sugars is described.

Carbohydrates↗
PubMed1977

Retrospective analysis of 645 simultaneous perineal punch biopsies and transrectal aspiration biopsies for diagnosis of prostatic carcinoma.

Transrectal aspiration biopsy of the prostate has been recommended as method of choice for morphological diagnosis of prostatic malignancies. It is less inconvenient to the patient and with hardly any complications. A retrospective analysis of 645 simultaneous perineal punch biopsies and transrectal aspiration biopsies was carried out in order to elicit whether prostatic carcinoma can be detected with transrectal aspiration biopsy as frequently as with perineal punch biopsy, and whether a cytological diagnosis of prostatic cancer is reliable. Carcinoma of the prostate was found in a total of 39.1% either with both techniques or with only one of them. A concordant diagnosis has been obtained in only 25.1%. Prostatic carcinoma was diagnosed more often by perineal punch biopsy (36.1%) than by transrectal aspiration biopsy (27.7%). However, the diagnosis has been missed by perineal punch biopsy in 2.6% of the cases. Doubtful results and unsatisfactory preparations have been observed more frequently after transrectal aspiration biopsies. Definite 'false-positive' cystological findings have not been detected. Thus, cytological evaluation of aspirated prostatic cells appears reliable. A definite morphological diagnosis can be expected with this technique. Simultaneous application of perineal punch biopsy and transrectal aspiration biopsy can be recommended in order to diagnose prostatic carcinoma with a higher frequency.

Biopsy↗
PubMed1976

[Imprint cytology. Advantages and possibilities].

Imprint cytology is a special variation of applied cytology, which can be used for quite different purposes. In scientific research cytologic imprints are very apt for cytophotometric measurements of DNA contents in nuclei as well as for karyologic studies, for instance on the different distribution of chromatin in the nuclei of benign and malignant tumors. Furthermore, imprint cytology is of great importance in teaching and learning results of aspiration biopsies, as the cellular patterns obtained with both methods are looking identical and moreover every imprint can be compared with the corresponding histologic slide. Last but not least in diagnostic procedures cytologic imprints are very helpful as an adjuvant to frozen and paraffin sections: They set off also very discrete changes and make possible diagnostic statements in a shorter time than it is possible with histologic slides.

Astrocytoma↗
PubMed1975

[On the sex chromatin and sex chromatin-like nuclear structures in human Purkinje-cells].

INTRODUCTION: Animal experiments have shown that in the course of chromatolysis the nuclear sex chromatin of nerve cells of female mammals changes its customary location at the nucleolus and migrates to the nuclear membrane; moreover, in the nuclei of nerve cells of males there occur under similar conditions nuclear structures which resemble the sex chromatin. It was, therefore, thought of interest to see if similar findings also apply to human Purkinje-cells. MATERIAL AND METHODS: The cerebellum of 100 female and 52 male corpses of various ages was examined. Paraffin sections, 2-5 mum thick, were stained with haemalumeosin and, after hydrolysis by hydrochloric acid, with cresyl violet of Schiff's reagent (Feulgen stain). In each cerebellum 100 Purkinje-cells, whose nuclei showed a well developed nucleolus and an obvious Barr body, were studied. In the cerebellum of 29 female corpses the cells examined showed a marked cytoplasmic chromatolysis; in the Purkinje-cells of the remaining 71 female, and of all the male corpses the stage of chromatolysis was classified according to the degree of dissolution of the Nissl bodies as "feeble", "marked", or as the stage of restitution, characterized by nuclear caps. RESULTS: The sex chromatin was seen as a, mainly, round and compact chromocenter with a maximal diameter of 0.6--1.6 mum. The larger Barr bodies were seen more frequently, if chromatolysis in the corresponding cells was marked. With increasing chromatolysis the Barr body, which usually occupies a perinucleolar location, tended to be found more often at or near the nuclear membrane. In the cerebellum of the males 17.4% of the Purkinje cells studied showed heteropyknotic nuclear structures which could not always be distinguished in size or in appearance from a sex chromatin. They occupied predominantly a perinuclear position. but with increasing chromatolysis they too were seen more frequently in a location which tended to be intermediary or near the nuclear membrane. Not infrequently the nuclei simultaneously presented a granular hyperchromatosis. DISCUSSION: The change in the location of the sex chromatin in the nucleus is considered to be associated with compensatory processes following chromatolysis. These in turn are accompanied by an intranuclear current of materials, such as RNA, towards the periphery of the nucleus, or by changed electric potentials which develop in the affected cells. The variations in size of the Barr body should be attributed to a variable condensation of the corresponding X-chromosome, rather than to polyploidy of the nerve cell nuclei. The sex chromatin-like chromocenters, which are seen in "male" Purkinje-cells in the course of chromatolysis, could be attributed to certain chromosomal segments which have a tendency to heterochromasia...

Cell Nucleolus↗