Biomedical subjects

H A Quinney

Publications and source records attributed to H A Quinney.

At least 19 recordsLinked to original sources

PubMed1992

Carnitine stimulation of glucose oxidation in the fatty acid perfused isolated working rat heart.

The effects of L-carnitine on myocardial glycolysis, glucose oxidation, and palmitate oxidation were determined in isolated working rat hearts. Hearts were perfused under aerobic conditions with perfusate containing either 11 mM [2-3H/U-14C]glucose in the presence or absence of 1.2 mM palmitate or 11 mM glucose and 1.2 mM [1-14C]palmitate. Myocardial carnitine levels were elevated by perfusing hearts with 10 mM L-carnitine. A 60-min perfusion period resulted in significant increases in total myocardial carnitine from 4376 +/- 211 to 9496 +/- 473 nmol/g dry weight. Glycolysis (measured as 3H2O production) was unchanged in carnitine-treated hearts perfused in the absence of fatty acids (4418 +/- 300 versus 4547 +/- 600 nmol glucose/g dry weight.min). If 1.2 mM palmitate was present in the perfusate, glycolysis decreased almost 2-fold compared with hearts perfused in the absence of fatty acids. In carnitine-treated hearts this drop in glycolysis did not occur (glycolytic rates were 2911 +/- 231 to 4629 +/- 460 nmol glucose/g dry weight.min, in control and carnitine-treated hearts, respectively. Compared with control hearts, glucose oxidation rates (measured as 14CO2 production from [U-14C]glucose) were unaltered in carnitine-treated hearts perfused in the absence of fatty acids (1819 +/- 169 versus 2026 +/- 171 nmol glucose/g dry weight.min, respectively). In the presence of 1.2 mM palmitate, glucose oxidation decreased dramatically in control hearts (11-fold). In carnitine-treated hearts, however, glucose oxidation was significantly greater than control hearts under these conditions (158 +/- 21 to 454 +/- 85 nmol glucose/g dry weight.min, in control and carnitine-treated hearts, respectively). Palmitate oxidation rates (measured as 14CO2 production from [1-14C]palmitate) decreased in the carnitine-treated hearts from 728 +/- 61 to 572 +/- 111 nmol palmitate/g dry weight.min. This probably occurred secondary to an increase in overall ATP production from glucose oxidation (from 5.4 to 14.5% of steady state myocardial ATP production). The results reported in this study provide direct evidence that carnitine can stimulate glucose oxidation in the intact fatty acid perfused heart. This probably occurs secondary to facilitating the intramitochondrial transfer of acetyl groups from acetyl-CoA to acetylcarnitine, thereby relieving inhibition of the pyruvate dehydrogenase complex.

Acyl Coenzyme A↗

PubMed1992

31P NMR of electrically stimulated rectus femoris muscle: an in vivo graded exercise model.

This study reports on the development of a model for studying skeletal muscle metabolism in humans using NMR spectroscopy. Graded exercise was simulated using electrical stimulation in 10 healthy, fit subjects (mean VO2max = 53 +/- 4 ml.kg-1.min-1). The effects of varying the stimulation parameters, namely, the stimulation frequency, the stimulation intensity, and the duty cycle, as well as the spectral interrogation volume, were compared using data acquired from the rectus femoris muscle. With stimulation, the inorganic phosphate to phosphocreatine concentration ratio ([P(i)]/[PCr]) and the intracellular pH both follow curvilinear relationships over the stimulation frequencies from 3 to 30 Hz, with the magnitude of the observed change related closely to stimulation intensity and duty cycle. Oxidative phosphorylation predominates at stimulation frequencies below 12 Hz, while anaerobic metabolism increases sharply above 12 Hz. Our findings show clearly the interdependence of the effects of the various stimulation parameters and emphasize the care that must be exercised in interpreting the physiological significance of the biochemical data obtained from electrical stimulation models used to study skeletal muscle metabolism.

Adult↗

PubMed1992

The effects of a reduced exercise duration taper programme on performance and muscle enzymes of endurance cyclists.

The influence of tapering on the metabolic and performance parameters in endurance cyclists was investigated. Cyclists (n = 25) trained 5 days.week-1, 60 min.day-1, at 75-85% maximal oxygen consumption (VO2max) for 8 weeks and were then randomly assigned to a taper group: 4D (4 days; n = 7), 8D (8 days; n = 6), CON (control, 4 days rest; n = 6), NOTAPER (non-taper, continued training; n = 6). Muscle biopsy specimens taken before and after training and tapering were analysed for carnitine palmityltransferase (CPT), citrate synthase, beta-hydroxyacyl CoA dehydrogenase (HOAD), cytochrome oxidase (CYTOX), lactate dehydrogenase, glycogen and protein. Significant increases in VO2max (6%), a 60-min endurance cycle test (34.5%), oxidative enzymes (77-178%), glycogen (35%) and protein (34%) occurred following training. After the taper, HOAD and CPT decreased 25% (P less than 0.05) and 26% respectively, in the CON. Post-taper CYTOX values were different (P less than 0.05) for 4D and 8D compared with CON. Muscle glycogen levels were increased (P less than 0.05) after tapering in the 4D, 8D and CON, but decreased in NOTAPER. Similarly, power output at ventilation threshold was significantly increased in the 4D (27.4 W) and 8D (27 W) groups, but decreased (22 W) in the NOTAPER. These findings suggest that tapering elicited a physiological adaptation by altering oxidative enzymes and muscle glycogen levels. Such an adaptation may influence endurance cycling during a laboratory performance test.

Adult↗

PubMed1992

Acute insulin withdrawal from diabetic BB rats decreases myocardial glycolysis during low-flow ischemia.

We have previously demonstrated that withdrawal of insulin treatment from BB diabetic rats for a 24-hour period will increase the failure rate of hearts subjected to low-flow ischemia. The purpose of this study was to determine if this increased severity of ischemia was related to a decrease in glycolytic rates during ischemia. Two groups of insulin-dependent diabetic BB Wistar rats were used; in one group, insulin treatment was withheld from rats 24 hours prior to study (uncontrolled), while in the second group, the daily insulin injection was not withheld (insulin-treated). Isolated working hearts obtained from these animals were perfused with 30 mmol/L (2-3H/U-14C)-glucose and 1.2 mmol/L palmitate, at an 11.5 mm Hg left atrial preload and 80 mm Hg aortic afterload. Hearts were subjected to a 15-minute aerobic perfusion followed by 60 minutes of low-flow ischemia (coronary flow, 0.5 mL/min). Under aerobic conditions, steady-state glucose oxidation rates (measured as 14CO2 production) were decreased in the uncontrolled group compared with the insulin-treated group (85.3 +/- 21.5 v 406.2 +/- 120.1 nmol/min/g dry weight, respectively; P less than .05). Steady-state glycolytic rates (measured as 3H2O production) were also decreased in the uncontrolled group compared with the insulin-treated group (1.73 +/- 0.30 v 5.57 +/- 1.26 mumol/min/g dry weight, respectively; P less than .05). During low-flow ischemia, glucose-oxidation rates markedly decreased in both groups (23.9 +/- 8.7 and 38.3 +/- 25.2 nmol/min/g dry weight in the uncontrolled and insulin-treated diabetic rats, respectively).(ABSTRACT TRUNCATED AT 250 WORDS)

Analysis of Variance↗

PubMed1992

Effect of high velocity resistance training on peak torque, cross sectional area and myofibrillar ATPase activity.

The purpose of this investigation was to determine the effect of high velocity resistance (HVR) training on peak torque (PT), cross sectional area (CSA) and myofibrillar ATPase activity of the knee extensors. HVR training was performed in a circuit on hydraulic exercise equipment, 4 times a week for 5 weeks at an angular velocity of approximately 3.14 rad.s-1. Knee extension PT was determined on a Cybex II isokinetic dynamometer and CSA of the quadriceps femoris muscle was assessed using computer tomography (CT) scanning. Muscle biopsies were obtained from the lateral quadriceps muscle and were analyzed for myofibrillar ATPase activity. Knee extension peak torque was significantly increased at 1.57, 2.09, 3.14, 3.66 and 4.19 rad.s-1. Myofibrillar ATPase activity and CSA was also significantly increased after HVR training. These findings showed that short-term high velocity resistance training enhances the in vivo torque/velocity curve especially at fast angular velocities and these changes are partly attributed to an increase in muscle CSA and activity of myofibrillar ATPase.

Adaptation, Physiological↗

PubMed1992

Changes in blood pressure and cardiac output during maximal isokinetic exercise.

This study was undertaken to determine the blood pressure (BP) and cardiac output (Qc) responses to maximal isokinetic exercise. The subjects (n = 5) performed unilateral knee extension/flexion exercise (knee exercise) and unilateral elbow extension/flexion exercise (elbow exercise) at 0.52, 1.57, and 2.62 rads.sec-1. The BP was monitored using a cannula placed in the radial artery. Heart rate (HR), stroke volume (SV), and Qc were measured by impedance cardiography. In response to isokinetic exercise, HR and Qc increased significantly (p less than .01), while the SV did not. The BP response was characterized by significant increases in systolic, diastolic, and mean arterial pressure (MAP) (p less than .01). The Qc and MAP, responses were not influenced by the exercise velocity. The adjustments in HR, MAP, and rate pressure product (RPP) to the elbow exercise were qualitatively similar to those seen during the knee exercise, but the absolute values achieved were smaller (p less than .05). Compared with maximal dynamic exercise, the HR and SV responses to the knee exercises were lower. The MAP response to isokinetic exercise equaled the highest value achieved during dynamic exercise. Findings from the present study suggest that the cardiovascular stress (the increase in HR, MAP, and RPP) associated with isokinetic exercise is independent of the velocity of movement and is proportional to the active muscle mass.

Adult↗

PubMed1991

Physiological adaptations to concurrent endurance training and low velocity resistance training.

This study investigated the effects of concurrent endurances and low velocity resistance training (LVR) on measures of strength and aerobic endurance. One group (ES) performed concurrent endurance training 3 days a week and LVR training on alternate days, 3 days a week for 12 weeks. The other group (S) performed only LVR training 3 days a week for 12 weeks without any endurance training. Measurements and increases in training volume were made every three weeks in both groups. Group ES exhibited increases in submaximal exercise responses after 3, 9 and 12 weeks (p less than 0.05). Knee extension peak torque and total work as well as cross-sectional area of quadriceps femoris were significantly increased after 6 and 9 weeks of training in both groups. These findings indicate that no significant differences in strength gains were observed between subjects performing concurrent endurance and resistance training or resistance training only. However, the time-course of adaptations between groups was somewhat different.

Adaptation, Physiological↗

PubMed1991

Adaptations to endurance and low velocity resistance training performed in a sequence.

The purpose of this study was to examine the effects of two different sequences of the same two training programs on strength and endurance adaptations. One group completed 5 weeks of low velocity resistance (LVR) training prior to 5 weeks of endurance training (Group SE) while the other group (ES) completed the opposite sequence. Endurance training and resistance training resulted in anticipated increases in aerobic endurance measures and strength, respectively, regardless of the sequence followed. Some maintenance of strength was observed during subsequent endurance training in Group SE. However, the improvements in peak VO2 and submaximal exercise responses that occurred after endurance training in Group ES were not maintained with subsequent LVR training. This investigation suggests that conducting LVR training prior to endurance training may be more beneficial if resistance and endurance training are performed in a sequence.

Adult↗

PubMed1991

Time-motion analysis of and heart rate responses to amateur ice hockey officiating.

The physical activity of referees (n = 4) and linesmen (n = 6) was analyzed from videotapes of four varsity hockey games to determine the frequency, average duration, and total time spent in each of six activity categories. The average duration of activity was low while frequency of occurrence was high. Low intensity activities occupied 96% of total time for referees and 94% of time for linesmen. Heart rate responses were recorded every 5 seconds and synchronized to the videotapes for all referees and 5 linesmen. Heart rates were above 70% of maximum for 70% of the total ice time, which was greater than expected for the observed physical activity. Psychological stress during the course of a varsity hockey game could contribute to the high heart rate responses. It is concluded that training to increase aerobic fitness is an important aspect of ice hockey officiating to increase resistance to fatigue and aid in stress management.

Alberta↗

PubMed1991

Effects of hydraulic circuit training following coronary artery bypass surgery.

The effect of hydraulic circuit training (HCT) on stroke volume (SV), cardiac output (Qc), aerobic power (peak VO2), and muscular strength and endurance was evaluated in 24 post-coronary artery bypass (CABS) patients (mean age = 52.8 +/- 2.6 yr). All assessments other than muscular strength and endurance were based upon a symptom limited graded exercise test on a bicycle ergometer. Muscular strength and endurance were assessed on a Cybex II isokinetic dynamometer. Sixteen patients were assigned randomly to 8 wk of cycle training or HCT (N = 8 in each). Subjects assigned to cycle training exercised on bicycle ergometers. The HCT group exercised on a three-station circuit, completing three circuits per day. Each circuit consisted of three 20 s work intervals at each station with a 1:1 work:rest ratio. Results from the training groups were compared with results from eight patients who served as a nonexercising control group. Following training the peak VO2 was significantly increased in the training groups (20% and 11% for the cycle and HCT groups, respectively; P less than 0.05). For both training groups, the increase in peak VO2 was associated with increases in SV and Qc and a reduction in heart rate (HR) at submaximal levels of exercise (P less than 0.05). Only the HCT group demonstrated an increase in both muscular strength and endurance during knee and shoulder exercises (P less than 0.05). These findings suggest that a program of HCT can elicit improvements in cardiovascular fitness and muscular strength and endurance in post-CABS patients.

Analysis of Variance↗

PubMed1991

Validity of convenient indicators of obesity.

A validation study of convenient indicators of obesity was undertaken in 540 male and female subjects, aged 7-14 yr. Four adiposity measures that have commonly accepted obesity classification points [relative weight, relative body mass index (BMI), sum of five skinfolds, and triceps skinfold] were derived from measures of height, weight, and five skinfold thickness measurements. Body density measures were converted to percentage of body fat using Lohman's (1986) age- and gender-specific regression equations. Using greater than or equal to 20% body fat for males and greater than or equal to 25% for females as the standard for obesity, the diagnostic utilities (sensitivity, specificity, overall accuracy, and positive and negative predictive values) of the four obesity indicators at their commonly used obesity cutoff points were determined. Preliminary analyses demonstrate that use of these indicators should not be considered independent of the gender of the subject or without reference to the purpose for classifying subjects as obese. Secondary analyses, in which the obesity cutoff point in each indicator was altered to obtain a minimum specificity level of 95%, demonstrated that a sum of skinfolds was better at identifying true obesity than all other indicators in both males and females. There is potential for inappropriate labeling with all convenient indicators of obesity, and thus they should be used with caution.

Adolescent↗

PubMed1990

Comparison of convenient indicators of obesity.

A comparative study of obesity measurements was undertaken with 533 male and female subjects, aged 11.8-15.9. Six adiposity measures (three skinfold indices, three height-weight indices) were derived from measures of height, weight, and eight skinfold thickness measurements. A principal components analysis of these adiposity measures resulted in a unifactorial solution accounting for 85.6% of the total variance. A cross-tabulation analysis with the derived factor scores and a criterion visual inspection rating supported the interpretation that the underlying construct of the factor was adiposity, and that a factor score of greater than 1.5 SD above the mean was a suitable standard for labeling obesity. Utilizing this dichotomy of factor scores as a standard, the differential diagnostic capabilities of four adiposity scales commonly used in identifying obesity was undertaken. Pursuit of this methodology, with the use of additional measures and larger sample sizes, is recommended to ensure the validation of an obesity measure.

Adipose Tissue↗

PubMed1989

The effect of velocity-specific strength training on peak torque and anaerobic rowing power.

This study investigated the effect of low- and high-velocity resistance training on isokinetic peak torque and anaerobic power output. Eighteen male varsity oarsmen were blocked on peak knee extension torque at 3.14 rad s-1 and assigned to a high-velocity resistance training group (HVR), a low-velocity resistance training group (LVR) or a control group. Subjects trained four times a week for 5 weeks. Each training session included three circuits of 12 stations using variable-resistance hydraulic equipment. The HVR training significantly improved peak torque (P less than 0.05) in knee extension and flexion at 2.61, 3.14, 3.66 and 4.19 rad s-1. The LVR training produced significant improvements (P less than 0.05) in peak torque for knee extension and flexion at 0.52, 1.05, 1.57 and 2.61 rad s-1. High positive correlations were found between peak torque and anaerobic power outputs for all groups. However, no significant changes occurred in 15 s power output, average 90 s power output or peak blood lactate in either training group. These results indicate that velocity-specific strength training does not necessarily improve anaerobic power output in a different exercise mode despite the high positive correlation between isokinetic strength and anaerobic power output.

Adult↗

PubMed1989

The influence of high-velocity circuit resistance training on VO2max and cardiac output.

In order to investigate the influence of high-velocity circuit resistance training on maximal aerobic power, maximal stroke volume and cardiac output, and blood lactate removal during recovery, 16 habitually active males were blocked on initial VO2max into either training or control groups. The training group completed two (weeks 1 and 2) or three (weeks 3-6) circuits of 10 variable-resistance hydraulic exercise stations at an exercise: relief ratio of 1:2 on alternate days over six weeks. Angular velocities of movement were maintained at approximately 3.1 rad.s-1. Following training, the VO2max was increased (p less than .01) from 4.32 to 4.68 1.min-1. Maximal stroke volume was increased (p less than .05) from 120 to 129 mL and heart rate response to an absolute submaximal exercise load was decreased (p less than .05) from 153 to 146 beats.min-1. As well, enhanced (p less than .01) removal of lactate from the blood was observed during recovery from exhausting exercise. No changes were observed for control subjects. These results indicate that positive alterations in aerobic and cardiovascular function may be achieved consequent to high-velocity circuit resistance training.

Cardiac Output↗

PubMed1988

Body fat assessed from body density and estimated from skinfold thickness in normal children and children with cystic fibrosis.

Body density and skinfold thickness at four sites were measured in 140 normal boys, 168 normal girls, and 6 boys and 7 girls with cystic fibrosis, all aged 8-14 y. Prediction equations for the normal boys and girls for the estimation of body-fat content from skinfold measurements were derived from linear regression of body density vs the log of the sum of the skinfold thickness. The relationship between body density and the log of the sum of the skinfold measurements differed from normal for the boys and girls with cystic fibrosis because of their high body density even though their large residual volume was corrected for. However the sum of skinfold measurements in the children with cystic fibrosis did not differ from normal. Thus body fat percent of these children with cystic fibrosis was underestimated when calculated from body density and invalid when calculated from skinfold thickness.

Adipose Tissue↗

PubMed1988

Sequencing of endurance and high-velocity strength training.

To compare two sequences of endurance (E) and high-velocity resistance (HVR) training, sixteen male oarsmen were separated into Group ES which trained endurance prior to strength and Group SE which trained strength prior to endurance. The endurance program consisted of up to 60 min a session, five days a week for five weeks. HVR exercise was conducted on 12 stations of variable resistance hydraulic equipment, four sessions per week for five weeks. Endurance training significantly improved VO2max and submaximal heart rate and blood lactate responses in both groups regardless of the sequence followed. HVR training improved VO2max in group SE only and had no effect on submaximal response to exercise. Peak torque increases for knee extension and flexion with HVR training were greater in group SE than group ES. These results show that organizing strength and endurance training into sequential programs can influence the physiological adaptation to training.

Adult↗

PubMed1988

Short-term cardiovascular adaptations to vertical head-down suspension.

Inversion devices have been advocated as means of therapy for patients with low back pain. The present investigation was undertaken to determine the cardiovascular responses to vertical head-down suspension with such a device. Eight men were monitored for three minutes in the upright and supine position, and for ten minutes in the vertical head-down position. Stroke volume (SV), heart rate (HR), cardiac output (QT), segmental arm blood flow (ABF), and segmental leg blood flow (LBF) were measured by impedance plethysmography. Moving from the upright to the supine positions resulted in an SV increase from 93.1 +/- 18.8 to 138.2 +/- 28.2 ml.beat-1 (p less than 0.01). During the first minute of suspension a further increase in SV to a maximum of 178.6 +/- 45.0 ml.beat-1 was noted. By the end of the suspension period, SV had declined to 159.6 +/- 34.2 ml.beat-1. Resumption of the upright position was associated with a reduction in SV to 94.3 +/- 15.8 ml.beat-1. Changes in QT across the supine and vertical head-down positions reflected the changes in SV. The vertical head-down position was also associated with a gradual reduction in both ABF and LBF. These data suggest that the primary effect of inverted suspension was a transient increase in venous return, which requires an effective ventricular response. An element of caution should be exercised in using these devices to avoid aggravating concurrent pathologic conditions.

Adaptation, Physiological↗

PubMed1987

Decrease in serum testosterone levels with maximal intensity swimming exercise in trained male and female swimmers.

Circulating testosterone levels have generally been reported to increase during short term, intense running or bicycle ergometer exercise. Swimming differs from such activity because of the strenuous arm activity and because it is conducted in a liquid medium in the horizontal position with the weight totally supported. To investigate whether such differences modify the serum testosterone response to exercise, we measured serum testosterone levels before and after a maximal intensity swimming test in ten elite male and ten elite female swimmers. Levels of circulating testosterone fell in 19 of 20 swimmers in contrast to previous reports of exercise in the vertical position. In women mean testosterone levels declined by 39.4% from baseline values and in males, mean testosterone levels declined by 19.0%. Since the incremental protocol was similar to in design to maximal intensity tests conducted on a treadmill or bicycle ergometer, these data suggest that the differing physical circumstances of swimming lead to a qualitatively different testosterone response to exercise.

Adolescent↗