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H A Schwartz

Publications and source records attributed to H A Schwartz.

16 recordsLinked to original sources
PubMed1984

Sequence analysis of derivatized peptides by high-performance liquid chromatography-mass spectrometry.

N-Acetyl-N,O,S-permethylated derivatives of oligopeptides were analyzed by high-performance liquid chromatography-mass spectrometry (HPLC-MS) using a moving belt interface. A heated-gas nebulizer was employed for sample deposition, thus permitting the effective use of a water-methanol gradient covering the range from 5% to 95% water at mobile phase flow-rates of 0.5 ml/min. We demonstrate in this paper that it is possible to sequence the octapeptide derived from the C-chain of glucagon by HPLC-MS analysis of a permethylated enzymatic hydrolysate of this peptide using overlap information from the mass spectral patterns. Moreover, it is shown that peptides not readily amenable to analysis by gas chromatography-MS can be analyzed using this approach. Preliminary results suggest that N-acetyl-methyl ester derivatives of oligopeptides may in specific cases also be a useful alternative for HPLC-MS analysis of complex oligopeptide mixtures.

Amino Acid Sequence↗
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Acquired Immunodeficiency Syndrome↗
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Lower gastrointestinal side effects of nonsteroidal antiinflammatory drugs.

It has been well accepted that the nonsteroidal antiinflammatory drugs (NSAID) can cause upper gastrointestinal (GI) blood loss and reactivation of peptic ulcer disease. This report describes 3 patients in whom the ingestion of a NSAID was temporally associated with serious lower GI disease, including bleeding in 2 patients. These cases suggest an association between NSAID and activation of serious lower GI disease.

Adult↗