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Biomedical subjects

H Anger

Publications and source records attributed to H Anger.

At least 37 records · Page 2Linked to original sources
PubMed1981

[Characterization of polygalacturonase covalently bonded to Sepharose].

For the soluble endo-polygalacturonase (EC 3.2.1.15.) from Aspergillus spec., investigated in the present work, the defined substrate turnover U at 50% loss of viscosity if 0.2% and is independent on the reaction temperature. In the case of the covalently-bonded enzyme, the following linear equation applies to U, depending on the specific activity A and in the limits from A = O [U] and Amax: U = [U] + S square root of A. U is influenced by the kind of linkage, the conditions of immobilization and the properties of the carrier: it is a measure of the postulated conformational change of the polygalacturonase. The characteristic limiting value for the substrate turnover at A = O [U] is also temperature-independent and proves to be a true increment of binding, whereas Amax depends essentially on the porosity of the carrier. Polygalacturonase-sepharose complexes with a real substrate turnover U of 3--20% were prepared by varying systematically the kind of linkage and the specific activity A. It was found that with increasing U these complexes were, as a rule, inhibited to a lesser extent by a non-competitive pectinase inhibitor than the soluble polygalacturonase. Furthermore, their ability to liberate or enrich oligomeric galacturonic acids with a degree of polymerization greater than 3 was markedly reduced.

Aspergillus↗
PubMed1978

[Mode of action and inhibition of polygalacturonase covalently bound to polysaccharide and glass carriers].

Endo-polygalacturonase (EC 3.2.1.15.) from Aspergillus spec. is much changed as far as its mode of action and the interaction with vegetable inhibitors of pectinase (from green beans and cucumbers) are concerned when it is covalently bound to insoluble carriers (Sepharose, cellulose powder, macroporous glass and nonporous ballotinis). Whereas a 2% degradation of substrate by the soluble enzyme caused a 50% decrease of viscosity of citrus pectic acid, the comparable degradation of substrate was increased to a level of about 10% with the investigated polygalacturonase carrier complexes apparently independent of the properties of the carriers and the kind of binding of the enzyme. In contrast to this the higher degradation of substrate of 15 and 20% respectively which was further stated at a 50% decrease of viscosity is unambiguously connected with the carriers and is in direct correlation with the specific activity of the polygalacturonase carrier complexes. Contrary to the soluble enzyme the covalently bound enzyme produces more lower oligomerous galacturonic acids by an exo-mechanism or by multiple attack already at the beginning of the hydrolysis of pectic acid. During the final stage there is an enrichment of trigalacturonic acid besides mono- and digalacturonic acids independent of the state of solution of the enzyme. It could further be stated that the strong inhibition of the soluble endo-polygalacturonase by selected pectinase inhibitors which was described earlier is reduced by degrees with the enzyme covalently bound to the insoluble carriers.

Enzymes, Immobilized↗
PubMed1977

[Social psychological aspects of suicide in the old age (author's transl)].

Referring to Emile Durkheim's classical appraoch the normative aspects of suicide are emphasized, regarding suicidal behavior not as a purely individual phenomenon but as always co-determined by the normative expectations of a person's social environment. Starting from the dramatic increase of suicide rates with age combined with a marked decrease in non-letal suicide attempts it is argued that normally suicide-inhibiting social norms become less effective as a person appraoches old age, thus weakening the appellative motivation components of suicidal behavior and strengthening evasive strivings. Results of 2 representative studies are reported which tend to support these as well as a number of more specific hypotheses. Moreover, some aspects in the attitude to younger persons to suicidal acts of old people seem even compatible with the assumption of latent suicide-facilitating expectations.

Adult↗
PubMed1977

[Umbilical cord AFP values in twins (author's transl)].

The concentration of alpha-fetoprotein (AFP) in the umbilical cord in twins was determined via radioimmunological analysis and compared with the values obtained in single births. Since a close correlation exists between AFP content and gestation age, this method can be used to estimate maturity. The mean concentration of AFP was significantly higher in the umbilical blood of fraturnal twins than in that of identical twins. Genetic factors could play a role here.

Female↗
PubMed1977

[Carcino-embryogenic antigen (CEA) in patients with genital tumors (author's transl)].

The concentration of carcino-embryogenic antigen in the serum of healthy women and of patients with different benign and malignant genital tumors was determined before and after treatment. The carcino-embryogenic antigen was elevated to more than 2.5 ng/ml in 11% of the healthy control patients. Patients with carcinoma of the breast had positive CEA tests in 60%. Patients with ovarian cancer had positive CEA tests in 57%, women with carcinoma of the uterine cervix had positive tests in 50% and women with endometrial carcinoma had increased CEA levels in the serum in 38% of the cases. Following treatment the CEA values decreased.

Breast Neoplasms↗
PubMed1976

Premature chromosome condensation in irradiated man.

Micronuclei and premature chromosome condensation previously described in mammalian cells exposed to different types of irradiation and chemicals in vitro or in vivo could be demonstrated in cultured lymphocytes derived from chronically low-dose irradiated persons. The usefulness of these findings for biological recording of irradiation effects is suggested.

Cell Nucleus↗
PubMed1976

[Spinning of rapeseed proteins].

Alkaline solutions of a mixture from rapeseed globulin and casein show plastic flow, which is influenced by protein concentration, relation of rapeseed globulin and casein, temperature and time of storage of the protein solution. The sodium hydroxide concentration with a great variability doesn't take effect on the rheological properties. With increasing content of casein in such alkaline solutions of rapeseed globuline and casein results an approach to NEWTONian flow. The spinnability of alkaline solutions of a mixture from rapeseed globulin and casein and the properties of the corresponding spun protein fibers are influenced by protein concentration, relation of rapeseed globulin and casein of the spinning solution and temperature of coagulating bath. The sodium hydroxide content of the spinning solution and the hydrochloric acid concentration of the coagulating bath with a great variability don't take effect. The spinnability of alkaline solutions of a mixture from rapeseed albumin starchsulphate and casein and the properties of the corresponding spun protein fibers compared to rapeseed globulin-casein mixtures are influenced additionally by sodium hydroxide concentration of the spinning solution and hydrochloric acid content of coagulating bath. The favourable influence on the properties of spun rapeseed albuminstarchsulphate-casein fibers isn't increased by a growth of the rapeseed albumin starchsulphate content more then 4% of the protein concentration of the spinning solution.

Albumins↗
PubMed1976

[Rheology and spinning of alkaline solutions of sunflower seed globulin and casein].

The specific demand of sodium hydroxide is determined for the dissolution of sunflower seed globulin, casein and a mixture of them to equal parts. In low protein-containing solutions it depends for sunflower seed globulin very much on the sodium chloride concentration. From sunflower seed globulin, casein and a mixture of them to equal parts are prepared with sodium hydroxide high protein-containing alkaline solutions. Sunflower seed globulin forms temporally a gel phase. After this phase the solution of sunflower seed globulin shows like casein and a mixture of sunflower seed globulin/casein (I:I) pseudoplastic flow. The flow curves of the pseudoplastic solutions are described mathematically with the OSWALDian power statement. By alkaline solutions of casein and sunflower seed globulin/casein (I:I) the flow exponent n is distributed statistically about 0.9, by solutions of sunflower seed globulin a distribution exists about the mean values n = 0.85 and n = 0.50. lg k depends in all protein solutions on the concentration of protein, sodium chloride, sodium hydroxide and on the temperature and time. For all protein solutions exists a linear relation between the logarithm of viscosity and the reciprocal temperature for lg k and I/T, which is derived normally for NEWTONian flow behaviour. In a suitable scope of spinning for all protein solutions are carried out complete factorial experiment, which guide to regression equations of lg k; in the case of sunflower seed globulin are calculated also a regression equation of the flow exponent n. Going out from the parameters of the spinning process the properties of the spun sunflower seed globulin/casein (I:I) fibers are described.

Caseins↗
PubMed1976

[Rheology and spinning of alkaline solution of field bean protein and casein].

From field bean protein and a mixture of field bean protein with casein to equal parts are prepared with sodium hydroxide high protein-containing alkaline solutions, which show pseudoplastic flow. The flow curves of the pseudoplastic field bean protein-casein (I:I)-solutions are described mathematically with the Ostwaldian power statement. In a suitable scope of spinning of this solutions are carried out complete factorial experiments, which guide to regression equations of lg k and n. g k depends on the concentration of protein; an effect of the sodium hydroxide concentration exists lonly about interactions. On the other hand the flow exponent n depends on the hydroxide concentration. The properties of the spun field bean protein/casein (I:I) fibres are described.

Caseins↗