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Biomedical subjects

H B Bosmann

Publications and source records attributed to H B Bosmann.

At least 181 records · Page 10Linked to original sources
PubMed1970

Synthesis of glycoprotein, glycolipid, protein, and lipid in synchronized L5178Y cells.

Synthesis of four macromolecular classes found in membranes-glycoprotein, glycolipid, protein, and lipid-was measured as a function of time of the cell cycle in synchronized L5178Y cells. Incorporation of leucine, choline, fucose, glucosamine, or thymidine into the cells, protein, nucleic acid, or lipid was measured by pulse-labeling for (1/2) hr at (1/2) hr intervals after release from the mitotic block. The amount of protein, lipid, glycoprotein, or glycolipid released or secreted into the medium by the L5178Y cells was also measured as a function of time of the cell cycle. Cellular protein was found to be synthesized throughout the cell cycle, with the highest synthesis occurring in the S period; synthesis was depressed in the M period. Cellular glycoprotein was synthesized at approximately the same times as protein, except that the rates of glycoprotein synthesis in the S period relative to other periods were much greater than for protein. Secreted protein was synthesized throughout the cell cycle without any general pattern, except that secretion was elevated in the late S and G(2) periods. Secreted glycoprotein was similar to secreted protein. Cellular lipid and cellular glycolipid were synthesized almost exclusively in the G(2) and M periods; there was no synthesis in the G(1) and S periods. Release or secretion of glycolipid and lipid also occurred in the G(2) and M periods.

Animals↗
PubMed1969

Mitochondrial autonomy: incorporation of monosaccharides into glycoprotein by isolated mitochondria.

Isolated intact mitochondria selectively incorporate monosaccharides from nucleotide diphosphate monosaccharides into protein. Fucose, mannose, glucose, and galactose were incorporated by the mitochondria into glycoprotein; xylose was not. Structural integrity of the mitochondria was not necessary for the incorporation of monosaccharide into glycoprotein; mitochondria broken by homogenization also incorporated monosaccharide. The monosaccharides incorporated into glycoprotein were localized in the inner mitochondrial membranes, the same membranes which contain the protein into which leucine is incorporated by the isolated mitochondria.

Animals↗