Biomedical subjects

H B Neustein

Publications and source records attributed to H B Neustein.

At least 37 records · Page 2Linked to original sources

PubMed1977

Ultrastructural abnormalities of liver cells in Reye's syndrome.

Electon microscopic observations were made on liver biopsy specimens from nine infants and children diagnosed as having Reye's syndrome by clinical, laboratory, and light microscopic criteria. In addition to excessive fat content, mitochondrial abnormalities were the most frequent abnormal finding in the liver. However, no correlation could be established between the severity of mitochondrial changes and clinical or biochemical data, and two patients with low levels of the first two urea cycle enzymes showed only mild mitochondrial abnormalities. Nonspecific or artifactual factors have been suggested to explain the mitochondrial changes. However, the data of this study suggest that most of the reported mitochondrial abnormalities are not artifacts, and that they can be helpful in the ultrastructural diagnosis of Reye's syndrome.

Adolescent↗

PubMed1977

Ferritin in human liver cells of homozygous beta-thalassaemia: ultrastructural observations.

Homozygous beta-thalassaemia is a disease in which there is a progressive iron overload from infancy to death in early adulthood. Liver biopsies from 10 patients in various stages of this disease were examined by electron microscopy. A number of round or oval lysosomal structures, containing lamellae different from myelin figures, were seen in all patients, including those with minimal iron overload. Ferritin molecules were seen either in relationship with the lamellae forming arrays, or in paracrystalline arrangement, or with no organized form. There were practically no ferritin molecules in sub-cellular compartments other than cell sap and lysosomes. The density of cell sap ferritin was constant beyond infancy, but the number of iron-laden lysosomes increased with age. The stages in the process of iron seclusion, seen even in advanced phases of iron overload, are described. Ferritin is thought to accumulate in lysosomes by a transmembraneous movement, but other explanations are considered.

Age Factors↗

PubMed1977

Morphology, growth, chromosomal pattern and fibrinolytic activity of two new human neuroblastoma cell lines.

Neuroblastoma cell lines LA-N-1 and LA-N-2 were extablished from neuroblastoma cells in the bone marrow and in the primary tumor, respectively, of two children with metastatic neuroblastoma. Morphology, growth in vitro and in athymic nude mice, chromosomal patter, and fibrinolytic activity of these cell lines and of previously extablished human neuroblastoma cell lines IMR-32, SK-N-MC, and SK-N-SH were compared. Most LA-N-1 cells were tear-drop shaped, small cells with processes; they tended to grow in clusters. LA-N-2 was comprised of elongated cells and small round cells, the latter growing in dense clumps on the former. Electron microscopy revealed numerous cytoplasmic dense cores in many LA-N-1 cells but none in LA-N-2 CELLS. During logarithmic growth in vitro, doubling times for LA-N-1, LA-N-2, SK-N-MC, SK-N-SH, and IMR-32 cells were 32,56, 23, 36, and 26 hr, respectively. Cells of all lines formed colonies in soft agar, and, after variable latency periods, LA-N-1, LA-N-2, SK-N-MC, and IMR-32 cells formed tumors in athymic nude mice. The marker chromosome(s) characteristic of each cell line was present in more than 90% of cells of given line. Significant plasminogen-dependent fibrinolytic activity was present in cells of all lines. These studies indicate that LA-N-1 and LA-N-2 cells arose from single but different aberrant progenitor cells and that they have properties of neuroblastoma cells. They also demonstrate that cell lines derived from human neuroblastomas are heterogenous as are the tumors in children.

Animals↗

PubMed1977