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H Bäumlein

Publications and source records attributed to H Bäumlein.

47 records · Page 3Linked to original sources
PubMed1990

A new transposable element in Chironomus thummi.

A 1.7 kb long transposable element called TECth1 was found in the 3' flanking region of a Chironomus thummi Balbiani ring gene. As shown by sequence comparison with a second copy, TECth1 is characterized by a perfect terminal inverted repeat of 17 bp flanked by a duplicated target site of 8 bp, four internal imperfect inverted repeats of 17 to 26 bp and terminal regions of about 0.25 kb with a high number of short direct repeats of the consensus sequence ACTTT or permutated and mutated forms such as TTTAC or ACTAT. The terminal inverted repeats and the 8 bp target site duplication are reminiscent of Drosophila P and hobo elements but no long open reading frame starting with ATG is present, suggesting that the two TECth1 copies studied represent deletion derivatives of a longer element coding for its own transposase. In situ hybridization revealed about 75 labelled sites distributed over all chromosomes with the Balbiani ring locus most strongly labelled. Fifty percent of the sites are specific for a given individual, and these variable sites are often heterozygous for the element.

Animals↗
PubMed1989

The legumin gene family: structure and evolutionary implications of Vicia faba B-type genes and pseudogenes.

We have characterized several Vicia faba genes encoding methionine residue-free group B subunits of the 11S or legumin storage proteins. The respective gene subfamily consists of 10 to 15 members, six of them having been studied by DNA sequence analysis. Four functional genes (LeB2, LeB4, LeB6, LeB7) are highly homologous in their coding region and 0.3 kb of their 3' flanking sequences. On the other hand, two pseudogenes (psi LeB1, psi LeB5) have accumulated a large number of mutations including an identical 0.7 kb internal deletion; they are both flanked by a repetitive element. Analysis of sequence changes show that transitions are nearly double as frequent as transversions. CpG is the most infrequent dinucleotide whereas TpA is significantly underrepresented in exon sequences. End points of deletions are correlated with short direct repeats and preferentially found in the two introns. Our studies indicate that the Vicia faba legumin B gene subfamily contains a group of expressed, highly homologous genes as well as more diverged pseudogenes.

Amino Acid Sequence↗
PubMed1986

The legumin gene family: structure of a B type gene of Vicia faba and a possible legumin gene specific regulatory element.

The field bean, Vicia faba L. var. minor, possesses two sub-families of 11 S legumin genes named A and B. We isolated from a genomic library a B-type gene (LeB4) and determined its primary DNA sequence. Gene LeB4 codes for a 484 amino acid residue prepropolypeptide, encompassing a signal peptide of 22 amino acid residues, an acidic, very hydrophilic alpha-chain of 281 residues and a basic, somewhat hydrophobic beta-chain of 181 residues. The latter two coding regions are immediately contiguous, but each is interrupted by a short intron. Type A legumin genes from soybean and pea are known to have introns in the same two positions, in addition to an extra intron (within the alpha-coding sequence). Sequence comparisons of legumin genes from these three plants revealed a highly conserved sequence element of at least 28 bp, centered at approximately 100 bp upstream of each cap site. The element is absent from the equivalent position of all non-legumin and other plant and fungal genes examined. We tentatively name this element "legumin box" and suggest that it may have a function in the regulation of legumin gene expression.

Amino Acid Sequence↗
PubMed1986

The 3' ends of two genes in the Balbiani ring c locus of Chironomus thummi.

The 3'-end sequences of two nonallelic genes derived from the Balbiani ring c (BRc) locus of Chironomus thummi are described. Only one of the genes appears to be transcribed abundantly in normal late larval salivary glands. The two sequences are highly similar, even in the 3' untranslated regions, but sharply diverge beyond the polyadenylation site. Together with evidence from the 3' ends of BR1 and BR2 genes of C. pallidivittatus and C. tentans, independently characterized by others, this result suggests the existence of a sequence-homogenization mechanism that operates across the 3' ends of all BR genes characterized to date. The 3'-terminal coding region of each BRc gene is divided into two portions by a short intron. The upstream portion is homologous to and continuous with the tandem repeats that make up the internal core of each BR gene; however, that portion is variant in sequence relative to the core, and apparently is not subject to the homogenization process that operates on the core repeats. The portion downstream of the intron encodes a unique, 111-residue polypeptide highly different from the rest of the BRc product. The evolution of the various segments of the BRc genes is discussed.

Animals↗
PubMed1984

Self-complementary DNA sequences within the BRc gene of Chironomus thummi.

The Balbiani ring c (BRc) DNA of Chironomus thummi consists of tandemly arranged 249-base pair (bp) repeats, which represent the major part of the gene (Bäumlein et al. 1982a) and are transcribed and translated in a periodic polypeptide of unusual size. To obtain further information on the DNA sequence organization of that gene a recombinant phage (lambda CthBRc-1) with a relatively long insert (containing predominantly 249-bp repeats) was studied by electron microscopy (EM). lambda CthBRc-1 was found to undergo specific sequence elimination of BRc DNA resulting in heterogeneous size distribution of insert length within the limits of the cloning capacity of the phage with a maximum around 15 kilobase pairs (kb). The EM analysis of R loops formed between recombinant molecules and poly(A)+RNA (containing the transcripts of BRc and BRb) revealed the existence of self-complementary inverted and direct repeats as further sequence elements of BRc DNA scattered throughout a long portion of the BRc transcription unit. Different intrastrand structures (stems, hairpins, complex loops) originate from the renaturation of several sets of self-complementary repeats. Most double-stranded regions fell into one main-size class with an average length of 0.1 kb. The overall data suggest that self-complementary repeats belong to the same DNA sequence family and are able to cooperate in the formation of loops of different size and complexity. The results are discussed in relation to the functional significance of self-complementary inverted repeats (palindromes) for BRc expression.

Animals↗
PubMed1984

Balbiani ring DNA: sequence comparisons and evolutionary history of a family of hierarchically repetitive protein-coding genes.

All known types of Balbiani ring (BR) genes consist of multiple, tandemly arranged, ca. 180 to 300-bp repeat units that can be divided into a constant region and a subrepeat region. The latter region includes short tandem subrepeats (SRs). Comparison of all available BR sequences using computer methods has enabled us (a) to define more precisely the constant and subrepeat regions, (b) to infer the evolutionary relationships among the various types of BR repeats, (c) to derive a consensus approximation of an ancestral sequence from a small segment of which the highly diverse present-day SRs may have originated, and (d) to detect an underlying substructure in the constant region, evident in the consensus but not in the present-day sequences and possibly corresponding to an original 39-bp DNA segment from which the extant, giant BR sequences may have evolved. We discuss the processes of reduplication, diversification, and homogenization within the hierarchically repetitive BR sequences as examples of how a simple DNA element may evolve into a diverse family of large, protein-coding genes.

Animals↗
PubMed1982

The basic repeat unit of a Chironomus Balbiani ring gene.

A clone derived from the Balbiani ring b (BRb) gene of Chironomus thummi has been used to study the internal organization of that gene. Much of the gene consists of approximately 80 copies of a ca. 300 bp repeat unit, which are tandemly organized. The BRb clone contains a major part of that unit (242 bp). Sequence analysis shows that approximately 60% of the unit corresponds to short, tandemly organized subsequences, which encode peptides 8 to 11 residues long. In turn, each subsequence consists of even shorter internal repeats, corresponding to a tripeptide (consensus Proline. Serine. Lysine.). The remainder of the ca. 300 bp unit probably does not have obvious repetitive substructure.

Animals↗
PubMed1982

Characterization of a 249-bp tandemly repetitive, satellite-like repeat in the translated portion of Balbiani ring c of Chironomus thummi.

A major part of Balbiani ring (BR)c DNA of Chironomus thummi consists of tandem 249-bp repeats which appear to be transcribed and translated into a polypeptide of very unusual composition. Whereas these 2549-bp repeats are evident by Southern blotting, sequence analysis reveals a finer tandemly repetitive substructure: more than half of the 249-bp repeat length consists of tandem 24-bp subrepeats , and these in turn may have been generated from even shorter sequences. Comparisons with partial BRb and BR1 sequences reveal that this hierarchically repetitive sequence structure is typical of BR genes. It resembles the structure of some satellite sequences, suggesting that mechanisms leading to satellite DNA evolution may also operate in the evolution of structural genes.

Animals↗
PubMed1980

Periodicities and tandem repeats in a Balbiani ring gene.

The Balbiani ring (BR) DNAs show prominent periodicities of restriction enzyme sites. Studies using a cloned fragment of the BRc gene strongly suggest that these periodicities reflect the existence of tandemly repetitive sequences within BR DNA. Tandem repeats measuring 54-58 bp have been demonstrated by partial sequence analysis of the BRc clone; the restriction site periodicities suggest the existence of additional 175 (= 3 X 58) and 1050 (= 6 X 175) bp repeat units. The short, medium and long repeats (58, 175 and 1050 bp, respectively) show sequence homology. Constrained unequal crossing over (resulting from misalignment of repeat arrays, usually by one repeat) is proposed as the mechanism for evolution of short, medium and long repeats from each other, in a manner analogous to evolution of satellite DNA sequences. Paradoxically, the dominant restriction site periodicities appear to be more conservative than might be expected on the basis of the overall sequence divergence between the sequenced repeats. This may be a consequence of functionally important, long-range amino acid or oligopeptide periodicities (for example, Asp x Ser or Glu x Ser corresponding to Hinf I sites) in the BRc protein product, in conjunction with preferential use of certain synonymous codons.

Animals↗
PubMed1976

RNA, RIBOSOMAL/*BIOSYNon of ribosomal 5S RNA genes in Chironomus thummi by in situ hybridization of iodinated 5S RNA.

5 S RNA of Chironomus thummi larvae was purified from total phenol extracted RNA by gel filtration and labelled to about 10(7) dpm/mug with carrier-free iodine-125. After hybridization in situ of 125I-5 S RNA and autoradiography only region B3c-e (containing two "normal" and two very faint bands) of chromosome II of salivary gland cells was highly labelled. In chromosomes of an animal showing pairing discontinuities a clearly "heterozygous" labelling of the 5 S RNA region was found. Region B3c-e shows no clearcut morphological signs of puffing or autoradiographically detectable 3H-uridine incorportion in spite of a continuous synthesis of 5 S RNA in salivary gland cells.

Animals↗