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Biomedical subjects

H Bürger

Publications and source records attributed to H Bürger.

At least 91 records · Page 5Linked to original sources
PubMed1984

Development and evaluation of a coculture technique for identification of anaerobic organisms.

We investigated a method to reduce oxygen tension and produce anaerobic conditions conveniently by coculture with a nonfermentative organism. The biochemical reactions of 94 strains representing 41 different species were determined using prereduced media and gas chromatography according to standard methods. The standard reactions were compared with those obtained by preparing similar media, modified by containing no cysteine or resazurin, under aerobic conditions. Preincubation of the modified biochemical media with a standard suspension of a culture of Acinetobacter lwoffi provided reducing conditions. In 1,449 individual tests using 22 different biochemicals, 1,349 (93%) of the reactions agreed. Only two misidentifications resulted when using the modified media, one each at the species and genus levels. Two strains of Actinomyces odontolyticus failed to grow in coculture.

Acinetobacter↗
PubMed1983

Genetic relatedness of hemagglutinins of the H1 subtype of influenza A viruses isolated from swine and birds.

The hemagglutinin (HA) gene of the influenza virus subtype H1N1 isolated from pigs and birds has been analyzed by the hybridization technique. According to the RNase protection data the HA genes of recent isolates from pigs in Northern Europe are genetically more closely related to those of isolates from birds in Europe and North America than to those of isolates from pigs in the United States, Taiwan, and Italy. Thus, two different H1N1 subtypes are circulating in the pig population. The results are consistent with the view that H1N1 viruses can be transmitted from birds to pigs and/or vice versa.

Animals↗
PubMed1982

Enhanced labelling of RNA synthesized in nuclei and reduced labelling of RNA synthesized in the cytoplasm by [32P]orthophosphate in the presence of nucleosides.

Labelling of cellular RNA by [32P]orthophosphate can be enhanced up to a factor of three by adding 2 to 10 X 10(-5)M of non-labelled nucleosides to the culture medium. The lag period of labelling can be reduced by a factor of two. Adenosine has the highest effect, while uridine is without effect. Labelling of viral RNA, which occurs in the cytoplasm, is reduced by nucleosides, suggesting that two precursor pools of nucleoside triphosphates are differently affected. The base composition of the labelled RNA cannot be influenced by incubation with non-labelled nucleosides. As a possible explanation for this observation a group translocation transport mechanism is discussed.

Animals↗
PubMed1981

[Single-dose gentamicin treatment and simultaneous localization of urinary-tract infections (author's transl)].

Single-dose gentamicin (80--120 mg; Refobacin) can be used successfully to treat significant bacteriuria in women, while at the same time differentiating between upper and lower urinary tract infection. Localization was achieved by demonstrating antibody-carrying bacteria in urine by immunofluorescence. In all 31 women with lower urinary-tract infection so treated urinary sample continued to be bacteria-free when retested one to for weeks later. On the other hand, in 22 of 25 women with renal bacteriuria the original bacteria were repeatedly isolated after treatment. Using single-dose gentamicin and subsequently re-testing urine samples, renal infections in four women were correctly diagnosed in this manner, although the initial immunofluorescence test was negative.

Antibodies, Bacterial↗