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Biomedical subjects

H Baurmann

Publications and source records attributed to H Baurmann.

At least 37 records · Page 2Linked to original sources
PubMed1982

8-methoxypsoralen and long ultraviolet effects on the rat lens: experiments with high dosage.

The effect of systemic 8-methoxypsoralen (8-MOP; 100 mg/kg daily) and subsequent long ultraviolet irradiation (UVA; 300 mJ/cm2; peak: 365 nm) on albino and pigmented rat eyes was studied in a 3-dimensional experimental set-up. While 8-MOP and UVA did not cause any ocular pathology when administered alone, a combined application of the two factors caused reversible corneal opacities, and irreversible iris devascularisation and cataracts. The irreversible changes were seen only in the albinos and accompanied by a significant decrease in lens wet weight. Phosphorescence and EPR spectroscopy demonstrated the formation of an 8-MOP-protein photoadduct in the animals treated with both 8-MOP and UVA. The results of this study emphasize the necessity of shielding the eyes of patients on photochemotherapy with protective spectacles.

Animals↗
PubMed1982

[Secondary fluorescence of intraocular vessels of the anterior segment of the rat eye after local drug application (author's transl)].

After local application of gentamycine, dexamethasone and tropicamide to rat eyes, no effect was observed on the permeability of the vascular wall of the iris and ciliary body compared with fluorescein-Na. Nor were there any changes in vessel caliber. The constriction of the dye column observed in corneal neovascularizations was not seen in the (normal) iris vessels and the vessels of the ciliary body. With regard to the iris region, however, this might be due to mechanical causes and/or the possibility that vessels of different origins react differently.

Administration, Topical↗
PubMed1979

[Behavior of fluorescein dye in the vascularized cornea of the animal eye after local application of certain drugs (author's transl)].

In pigmented rabbits we investigated the much-used drugs pilocarpine, atropine, and tropicamide (Mydriaticum, Roche) with regard to their possible influence on the permeability of newly formed corneal vessels. Sodium fluorescein was chosen as test substance. Corneal vessels were produced by introcorneal injection of 0.1 N NaOH. Their development required 2--3 weeks and their growth about 4--6 weeks. A 2-week period of stability followed and was used for the local application of the above drugs. An evident influence on the diffusion of fluorescein could not be ascertained. Tropicamide induced a narrowing of the dye column in the corneal vessels. This phenomenon may be due to a narrowing of supplying vessels. In all cases, dye diffusion took place especially at the peripheral loops of the corneal vessels.

Animals↗
PubMed1979

[Differences in permeability for FITC-dextrane in cornea neovascularisation (author's transl)].

3 weeks after NaOH cauterization of rat corneas, the newly formed vessels were angiographically investigated by means of FITC dextran fractions. An angiographic picture similar to that of fluorescein Na was observed after intravenous administration of FD 3. A minimum diffusion of FD 40 could only be detected in the area of the distal terminal loops of the newly formed corneal vessels. FD 70 and FS 150 shoed an angiographically similar behaviour with no diffusion in the whole area of neovascularization.

Animals↗
PubMed1978

[Limited diffusion of dye between choroid and retina during animal experiments after injection of fluorescein and rhodamin (author's transl)].

For contributing to the question of an existence of a diffusion of the unbound part of the fluorescein between choroid and retina in fluorescein angiography, on pigmented rats, after intravenous injection of Fluorescein sodium and Rhodamine B respectively, comparing observations were effectuated. Rhodamine B in its unbound form showed a distinct diffusion between choroid and retina and coloured all retinal layers in the late phases whereas in the case of the Fluorescein sodium, only a poor diffusion was observable. The similar spectral bands of the primary and secondary fluorescences render difficult the observation of the behaviour of the fluorescein dye.

Animals↗
PubMed1977

[Regression of corneal neovascularization by laser treatment? (author's transl)].

The corneal neovascularization produced by NaOH burns was examined in two groups of pigmented rabbits following laser treatment. The treatment was carried out with energy level 500 mW, spot diameter 200 micron, and exposure time 1 s throughout. In the first group, a single newly formed vessel was coagulated in each case. Subsequent fluorescein angiography invariably showed an incomplete occlusion of the vessel. In the second group, we coagulated a section of the neovascularization network at its origin in the corneal limbus. After 48 h, fluorescein perfusion was once again observed, but the vessels were predominantly finer than before. Fluorescence microscopy revealed a rich neovascularization of the more superficial layers of the corneal stroma. To be successful, laser treatment must involve both supplying and draining vessels.

Animals↗
PubMed1976

[Experimental fluorescein angiographic and microscopic investigations on laser treated rat eyes (author's transl)].

On fundi of pigmented rats, with Argon laser constantly 300 mW of power. 2 seconds exposure time and 50 microns spot diameter, coagulations have been performed. The eyes have been observed up till 3 months after the intervention, then angiographies were performed, and the following enucleated eyes have been examined with the fluorescence microscope. Neovascularizations and dye diffusions were observable mainly in the marginal zones of the arised cicatrices. The neovascularizations were starting from the choroid and penetrating into the outer retinal layers.

Animals↗
PubMed1976

Observation of injected fluorescein diffusion after laser treatment of cat fundi. An experimental study with angiography and microscopy.

Argon laser coagulations with a power of 100 mW and 300 mW, exposure time 0.02 and 0.02 sec, and constant spot size of 100 microns were applied on normal cat fundi. From 1 and 2 hrs to 7 days after the coagulation, the treated eyes were observed angiographically and by fluorescence microscopy after dye injection; freeze dried eyes were examined under a fluorescence microscope. Abnormal dye leakages originated from the chorid and from retinal vessels were detected in the specimens examined within 72 hrs after the coagulation.

Animals↗
PubMed1976

[Occurrence of diffusions shown by fluorescence angiography of the laser coagulation in animals (author's transl)].

Laser treated rat fundi were observed angiographically and with the fluorescence microscope for 3 months. Angiographically diffusions have been observable up to about one week post laser, fluorescence microscopically, however, for the whole observation time of 3 months. From the 2nd week, these diffusions were produced mainly by neovascularizations which angiographically were not visible. They started from the choroid and penetrated into the outer part of the retina.

Animals↗
PubMed1975

Investigations on laser coagulated rat eyes by fluorescence angiography and microscopy.

Laser coagulation using 50 and 100 mW of power, a spot of 50 microns and an exposure time of 0, 2 seconds were performed on rat eye fundi. We observed the effects which were produced in the retina and choroid utilizing both fluorescence photomicroscopy with incident excitation light and fluorescence angiography. No distinct differences were noticed between the effects produced by 50 and by 100 mW. Both energies damaged choroid and retina, especially the outer part of the retina. Moreover, fluorescein angiography within 24 hours gave us effective information for estimating the applied laser effect.

Animals↗