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H Beukers

Publications and source records attributed to H Beukers.

At least 19 recordsLinked to original sources

Who made John Snow a hero?

This paper describes how and why John Snow's investigation of the transmission of cholera grew into an epidemiologic classic. The evolution of the interpretation of the work of John Snow was first studied in depth in the Dutch medical literature, and thereafter traced more superficially in the bacteriologic, hygienic, and epidemiologic literature of Germany, the United Kingdom, and the United States. From the oral tradition of teaching, as well as from the written sources, it is concluded that US epidemiologist W. H. Frost was responsible for the revival of the work of John Snow in the 1930s. Besides the obvious and enjoyable clarity of thinking and reasoning, epidemiologically and medically, of the writings of John Snow, his example well suited epidemiology of the 1930s since his convictions came very close to the bacteriologic paradigm of the day.

Cholera↗

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Academies and Institutes↗

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History, Modern 1601-↗

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History, Modern 1601-↗

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Biochemistry↗

Analysis of methotrexate by isotachophoresis.

This paper shows that the anion of methotrexate (MTX) can be readily separated and quantified by isotachophoresis. An extraction method for MTX is also presented, appropriate for isotachophoretic studies. The extraction of MTX is based on the complexation and precipitation of MTX with metal ions. The recovery of MTZ from plasma is about 75%.

Anions↗

Latex phagocytosis by polymorphonuclear leukocytes: role of sialic acid groups.

Polystyrene latex particles are rapidly phagocytized by rabbit polymorphonuclear (PMN) leukocytes. The uptake is influenced by macromolecules which have the effect of altering the surface charge of the latex particle. The influence of polylysines of varying chain length on the surface charge of latex particles and of PMN cells was studied by micro-electrophoresis. Charge reversal at the latex surface was found to occur at concentrations considerably below that at which the surface charge of the PMN cells is reversed. Phagocytosis of latex by PMN cells is enhanced in the presence of low concentrations of long-chain polylysines. The enhancement of phagocytosis is strongly reduced if PMN cells are treated with neuraminidase. This suggests participation of sialic acid groups in a stage of particle-cell interaction which precedes engulfment.

Animals↗

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Academies and Institutes↗

Effects of albumin on the phagocytosis of polystyrene spherules by rabbit polymorphonuclear leucocytes.

Polystyrene latex spherules are rapidly phagocytized by polymorphonuclear leucocytes. Surface modifications of the latex spherules can lead to an inhibition of phagocytosis, and the purpose of this study was to analyse in particular inhibition by bovine and human serum albumin. It can be shown that albumin is bound much more strongly by the PSL particle surface than by the cell surface. Furthermore, a correlation was established between the extent to which the particle surface is covered and the extent of phagocytosis inhibition. In the binding of albumin to the particle surface ionic as well as hydrophobic interactions play a role. As a result, the particle acquires a negatively charged hydrophilic coating which effectively diminishes the number of effective cell-particle collisions leading to engulfment.

Animals↗

Phygocytosis by rabbit polymorphonuclear leukocytes: the effect of albumin and polyamino acids on latex uptake.

Albumin in low concentrations (0.0001-0.01 weight percent) was found to be an effective inhibitor of phagocytosis of polystyrene latex beads by rabbit polymorphonuclear leukocytes. Polyglutamic acid proved to be an inhibitor of latex uptake at even lower concentrations. Polylysine stimulates phagocytosis, maximal stimulation occurring at 0.002% polylysine. These findings are discussed with reference to the surface properties of latex particles and leukocytes, and particularly with reference to electrostatic interactions in phagocytosis.

Glutamates↗

History of cancer research in nosological perspective.

Contrary to conventional wisdom progress in cancer research has not followed a linear course, but rather a torturous capricious path, often guided by the prevailing concepts of disease (nosology). Various theories appear, disappear and reappear all the time. Periods with rapid progression alternate with periods of stagnation. Really new ideas emerge rarely in oncology. Most of the so-called new ideas are modifications of older concepts i.e. old wine in new skins. Moreover existing oncological theories brought under the umbrella of a new nosological paradigm may change drastically and often get a totally new meaning. As a consequence older theories may linger on over centuries but may be modified to such an extent that the original concepts are barely recognisable. Progress in oncology is characterised by periods of rapid progress alternating with periods of apparent stagnation which with hindsight were nevertheless periods in which new concepts were in statu nascendi. The history of oncology provides still a source of inspiration, since older ideas may be worth reconsidering.

History, 20th Century↗