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H Bockendahl

Publications and source records attributed to H Bockendahl.

13 recordsLinked to original sources
PubMed1978

[Psoriasis and bullous dermatoses].

In the last five years we observed in Berlin seven cases of psoriasis vulgaris combined with bullous eruptions of the pemphigoid type. Exogenious factors provoking the bullous eruptions were sun exposure, photochemotherapy and treatment with dithranol. These factors did not cause contact allergy of type IV, but pemphigoid eruption which could be characterised by immunfluorescence. This, was accompanied in two cases with high numbers of eosinophils in tissue and blood. The results show similarities with the findings of Person and Rogers who have shown the manifestation of otherwise subclinical pemphigoid in patients being treated for psoriasis.

Adult↗
PubMed1977

Complement as an essential factor for indirect immunofluorescence in cases of pemphigoid.

Hitherto in the literature no proof has been published of a complement activation on the basement membrane in cases of pemphigoid. In this paper, for the first time, it is demonstrated, even if only in vitro, that complement, whose presence in the region of the basement membrane has long been known, plays an active role in a reaction. Through the action of the haemolytic system (amboceptor and sheep erythrocytes) and of anti C3 on reaction specimens for indirect immunofluorescence in pemphigoid, it is shown that the indicator reaction of the fluorescein-labelled anti-IgG with the immune complex attached to the basement membrane is markedly dependent on complement. Free, dissolved IgG is precipitated by appropriate anti-serum, even without complement.

Antigen-Antibody Complex↗
PubMed1977

Investigations on the binding sites of the basement membrane zone for pemphigoid antibodies in vitro. II. Immunohistochemical reactions of the reactive groups.

In continuing our studies on the characteristics of the antigenic structures of the basement membrane, procedures used in histochemistry for the identification and blocking of reactive groups have been adapted in this present work to the pretreatment of cryostat sections of guinea-pig tongue. These sections have then been studied by the indirect immunofluorescence method. By means or reagents, which produce a reversible extinction of the basement membrane fluorescence produced with pemphigoid sera, it proved possible to determine the existence of certain chemical groupings which bind the anti-basement membrane antibodies, namely: free carboxyl groups deriving from amino acids, hydrogen bridges in which the hydroxyl groups from the carbohydrates in the antigen are probably involved, bonds involving the free aldehydes of the antigen (either cross-links from aldehydes, or formation of Schiff's bases with amino groups of the antibodies), and lastly, to a lesser extent, disulphide bonds. The antigen/antibody reaction probably occurs in the neighbourhood of the alpha-glucosido-beta-galactosido-hydroxylysine. A reaction model has been developed from these findings. There were also some indications that the antigenic structures of the intercellular substance are chemically related to those of the basement membrane. The cell nucleus antigens, on the other hand, appear to possess different chemical properties.

Animals↗
PubMed1976

[Antibody titers--curse in patients with recurring Herpes simplex virus infections under vaccination with heat-inactivated Herpes viruses].

Follow-up studies of antibody-titres in patients suffering from recidivations of herpes labialis and progenitalis treated with herpes virus vaccine inactivated by heat. It was stated by several authors that patients suffering from recidivations of herpes labialis and progenitalis can be successfully treated with a herpes virus vaccine inactivated by heat (Lupidon G/H). Nothing is known, however, about the mechanisms diminishing recurrences. We have studied 11 patients and 3 control persons whether there were changes in specific antibody titres after repeated vaccinations. Skin reactions following intradermal injections were also tested. Serum samples studied over a period of 8 weeks until 9 months showed that the antibody titres found by complement-fixation and neutralization tests remained practically constant. Skin tests were always negative. Therefore it merits further study to find out the mode of action of this clinically successful treatment.

Antibodies, Viral↗
PubMed1976

The activation of antigens of the basement membrane zone by proteolytic enzymes in vitro.

Following brief exposure of cryostat sections of human skin to the action of proteolytic enzymes (papain or trypsin), there was a pronounced increase in the antibody binding sites of the basement membrane zone, shown in indirect immunofluorescence by an increased intensity in fluorescence of the basement membrane zone as compared with preparations which had not previously undergone incubation, and by an increase of 5-6 (papain) or 4-5 (trypsin) titre dilution steps. This effect was practically absent when guinea-pig tongue was used as the antigenic substrate. In conjunction with findings published in the literature, our results can be interpreted as indicating that the activation of the basement membrane zone antigens by proteolytic enzymes is associated with an increase in antigenicity which results in the formation of "auto-antibodies" of the basement membrane zone antibody type.

Antibody-Producing Cells↗
PubMed1976

[Stimulation of autoantibody formation in pemphigoid due to proteolytic enzymes?].

Following brief exposure of cryostat sections of human skin to proteolytic enzymes (papain or trypsin), there is an increase in the antibody binding capacity of the basement membrane zone for basement membrane zone antibodies of patients with bullous pemphigoid. In conjunction with findings published in the literature, our results indicate that activation of the basement membrane zone antigens by proteolytic enzymes is associated with an increase in antigenicity which results in the formation of auto-antibodies of the basement membrane zone antibody type.

Antibody Formation↗
PubMed1975

[The mechanism of antigen-antibody reaction at the basement membrane zone in bullous pemphigoid. The question of interaction of complement factors and bivalent cations (author's transl)].

1. In a medium poorly cationic indirect immunfluorescence of the basement membrane zone normally observed with preparations of sera from patients with pemphigoid has not be seen. 2. The fluorescence of basement membrane zone takes place if Fe2+ ions are added together with the antibasement zone antibodies. Evidently Fe2+ ions are essential for fixing antibodies. 3. With the method of indirect fluorescence the C3 component of complement could be observed after fixation of pemphigoid antibodies to the basement membrane zone. 4. EDTA prevents binding C3 too. Only after adding Fe2+ (not with Ca2+ or Mg2+) complement IF staining is demonstrable. Apparently EDTA causes the loss of fixing antibasement zone antibodies at first. Thereafter complement staining reaction is negative. 5. Participation of C1 could not be demonstrated using indirect IF staining. The alternate pathway to activation of C3 is discussed.

Antigen-Antibody Reactions↗
PubMed1975

Immunofluorescent follow-up studies in patients with bullous dermatoses.

Basement zone antibody titres were studied in two patients with bullous pemphigoid over a period of 1-1/2 and 3-1/2 years. Titres remained almost constantly even after a complete healing of all lesions, without any treatment. Direct immunofluorescence revealed basement zone antibodies in one of these two patients when examined after healing had occurred. Three patients with bullous dermatoses showed pemphigus antibodies at the inception of the disease. Basement zone antibodies were present simultaneously in one patient. In the subsequent course of observation in the two other patients, pemphigus antibodies were replaced by basement zone antibodies and in one case by nuclear antibodies too. These findings make the pathogenetic import of these "auto-antibodies" difficult to understand.

Aged↗
PubMed1975

The effects of X-ray, ultraviolet and infrared irradiation on the basement membrane zone antibody reaction of the human skin in vitro.

Two patients with bullous pemphigoid were treated with radio cobalt or betatron irradiation. Thereafter bullous eruptions developed in the non-irradiated skin. It is known from the literature that ultraviolet light applied to the normal-appearing skin of patients with bullous pemphigoid produced fresh histologic and immunohistochemical changes in the irradiated skin. X-ray irradiation applied to human skin biopsy specimens using single doses of 7 000 and 8 000 R resulted in an increase of basement membrane zone antibody binding of 2-3 titre dilution steps. An increase of 3-4 titre dilution steps was achieved by irradiation with ultraviolet light. Infrared irradiation of skin biopsy specimens, on the contrary, caused the total loss of the basement membrane zone fluorescence. It merits further study, whether these contrary effects on the basement membrane zone antibody binding of human skin in vitro (activation of bound antigen after X-ray or ultraviolet irradiation and inactivation of antigen caused by infrared irradiation) are of clinical value concerning the bullous pemphigoid problem in vivo.

Aged↗