PubMed Health⌕ Search

Biomedical subjects

H Bos

Publications and source records attributed to H Bos.

At least 19 recordsLinked to original sources
PubMed2001

Pharmacological considerations during pregnancy.

We present a brief overview of recent literature concerning some of the drugs used in pregnancy, labour and delivery. Obstetric anaesthesia continues to evolve through the reuse of old drugs such as magnesium sulphate or the manipulation of current drugs (e.g. propofol for emesis) in order to improve patient outcome. Pregnant women have traditionally been therapeutic orphans. The use of new agents such as levobupivacaine and ropivacaine in obstetric patients lags behind that of their non-pregnant counterparts. However, this gap is decreasing and these new drugs offer benefits to the parturient woman.

Journal Article↗
PubMed2000

Phagocytosis of yeast: a method for concurrent quantification of binding and internalization using differential interference contrast microscopy.

In studies of phagocytosis there is a need to distinguish targets that are internalized by the cell from those that are bound to the cell surface. The present work describes a simple method by which internalized and surface-bound yeast particles can be identified by differential interference contrast microscopy, using trypan blue to stain surface-bound yeast particles. The method has the advantage that both internalized and surface-bound particles can be visualized without the need to switch the illumination source and/or filter sets, thus facilitating concurrent quantitation of binding and internalization. The method was evaluated with the phagocytosis-modulating agents horseradish peroxidase (HRP) and cytochalasin D, using adherent resident macrophages as phagocytic cells. When macrophages are challenged with a particular type of target, they usually bind many more targets than they ingest. It was shown that yeast particles were arrested in the initial binding phase of phagocytosis depending on the region of macrophage plasma membrane where binding sites were formed. Failure of surface-bound yeast particles to trigger internalization was not due to modifications of the yeast particle surface. Nor was it due to binding to non-phagocytic receptors, or low-affinity receptor-ligand interactions. The glycoprotein HRP inhibited only the binding stage of phagocytosis, whereas cytochalasin D, a drug that affects actin polymerization, inhibited both binding and internalization. However, when the yeast particles were pre-incubated in fresh mouse serum, cytochalasin D inhibited only the internalization step. The assay described here may be useful in studies concerned with the function and expression of phagocytosis-mediating surface lectins.

Animals↗
PubMed2000

Role of patient factors in therapy resistance to antiproteinuric intervention in nondiabetic and diabetic nephropathy.

Reduction of proteinuria is a prerequisite for successful long-term renoprotection. To investigate whether individual patient factors are determinants of antiproteinuric efficacy, we analyzed individual responses to different modes of antiproteinuric intervention in nondiabetic and diabetic patients, obtained in prior studies comparing the efficacy of various pharmacological regimens. The individual antiproteinuric response to angiotensin-converting enzyme (ACE) inhibition positively correlated to the response to angiotensin type I (AT1) receptor blockade in diabetic (r = 0.67, P < 0.01, N = 16) as well as nondiabetic patients (r = 0.75, P < 0.01, N = 12). This corresponded to the correlations for antihypertensive efficacy between ACE inhibition and AT1 receptor blockade in diabetic (r = 0.73, P < 0.001) as well as nondiabetic patients (r = 0.55, P < 0.05). Remarkably, the antiproteinuric response to ACE inhibition also correlated positively to the antiproteinuric response to indomethacin (r = 0.63, P < 0.05, N = 9). Thus, patients responding favorably to one class of antiproteinuric drugs also respond favorably to other classes of available drugs, supporting a main role for individual patient factors in responsiveness or resistance to antiproteinuric intervention. In the search for strategies to improve response in these high risk patients, combination-treatment (combining different drugs, and combining drugs with dietary measures like sodium and protein restriction), and the use of higher doses may provide more fruitful strategies to optimize renoprotection than shifting to other classes of the available drugs.

Angiotensin-Converting Enzyme Inhibitors↗
PubMed1996

Surfactant nebulization does not alter airflow obstruction and bronchial responsiveness to histamine in asthmatic children.

To test the hypothesis that surfactant nebulization improves pulmonary function and reduces bronchial responsiveness to histamine, we studied 12 children with asthma. We measured before and after inhalation of 100 mg nebulized natural-derived bovine surfactant (Alveofact (registered)) and after nebulization of 0.9% NaCl the change in peak expiratory flow, vital VC, FEV1, and forced expiratory flows at 50 and 75% of the flow-volume curve. In addition, we performed a histamine inhalation challenge. We did not find any significant changes in these parameters after nebulization of surfactant. These findings indicate that the inhalation of nebulized surfactant does not alter airflow obstruction and does not alter bronchial responsiveness to histamine in asthmatic children with airflow obstruction.

Adolescent↗
PubMed1994

Methylphenidate and information processing. Part 1: Differentiation between responders and nonresponders; Part 2: Efficacy in responders.

Part 1 of this study attempted to discriminate clinical responders and nonresponders to methylphenidate (MPH) on the basis of neuropsychological deficit profiles. Part 2 addressed the question to what extent MPH might ameliorate these deficits. Hyperactive clinical responders (n = 30) and nonresponders (n = 28) to MPH, were compared to normal controls (n = 27) on selective and sustained attention tasks and on conventional psychological and neurological measures. The responders took part in a randomized double-blind placebo-controlled crossover study. They ingested a placebo (PL) or MPH (0.3-0.6 mg/kg/day) during 4 weeks, and were then tested. After crossover, another period of 4 weeks was concluded by a second test series. Compared to controls, both responders and nonresponders showed attention deficits in encoding, memory search, and decision operations, as well as in focused and sustained attention, vigilance, and use of feedback. These deficits were the most severe in the responders. Anamnestic data, IQ scores, and neurological variables did not discriminate between groups. MPH selectively alleviates attention deficits. MPH did not affect divided attention, except for an increased accuracy of response organization. The drug, however, greatly improved focused and sustained attention, vigilance, impulsivity, and the behavioral adaptivity to feedback.

Attention↗
PubMed1986

Heredity of navicular disease.

Bone structure, nutrient canals, form and definition, and a total evaluation of both fore navicular bones were radiologically evaluated in 169 three- and four-year-old Dutch warmblood mares divided into eleven daughter (half sister) groups. On the basis of the 'total evaluation', differences between the three- and four-year-old horses were detectable. Using the 'total evaluation', significant differences were found between daughter groups consisting of three-year-old mares. These differences support the theory that podotrochleosis may be (partly) due to the presence of genetic factors. The possibilities concerning selection are presented. The results of the radiological evaluation of the stallion do not have predictive value as far as his progeny is concerned.

Animals↗
PubMed1984

[The hygienic status of meat transport vans].

Meat transport vans were sampled using impression surfaces of violet red bile glucose agar (VRBG agar; in 1978) and plate count agar (PC agar) contained in so-called Rodac plates (during the period from 1979 to 1982). So-called hygienograms of each van were recorded. At the same time, scrapings from the vans were examined for contamination by Salmonella. The majority of meat vans were only slightly contaminated with Enterobacteriaceae. The state of hygiene of approximately fifty per cent of the meat vans examined with plate count agar was inadequate initially. However, considerable improvement occurred during the course of the investigations. Seasonal effects were merely limited. Moreover, there was not found to be any relationship between the appearance of scrapings positive for Salmonella and the state of hygiene of the transport vans. Systematic bacteriological examination of meat transport vans is considerably hampered in practice by the ambulatory character of meat transport.

Animals↗
PubMed1980

[Genetics and latent defects in animals. II. Microphthalmia in sheep (author's transl)].

In this second clinical paper, the genetic features of the case of microphthalmia in sheep reported by Wagenaar is discussed in greater detail. The situation is compared with that on breeding other animal species in the Netherlands. Probability calculations based on the situation described are carried out. The replies by experts appointed by the Court of Justice are commented upon.

Animals↗
PubMed1975

[Preliminary performance test in saddle horses (author's transl)].

The study of the literature on a preliminary performance test for saddle horses (stallions) is concerned with European countries. Interest is found to be increasing in the various countries. Performance of the test in the Netherlands (since 1966) is described and the various criteria of evaluation are discussed in detail. The intensity of selection based on this test is referred to. In 1974, it was 78 per cent in the Netherlands. Part of studies on the relationship between some results of testing are reviewed. The traction test shows a very low coefficient of correlation with the other parts of the examination. The contemplated analysis of the results of progeny groups is discussed. In the comment, the fact is stressed that there should be more international co-operation in this field. Finally, the preliminary performance test is considered as part of the criteria adopted in the selection of saddle horses in the future.

Animal Husbandry↗
PubMed1974

[Work in Papakura].

Explore the source record for details and available documents.

Foreign Professional Personnel↗