Biomedical subjects

H Boye

Publications and source records attributed to H Boye.

7 recordsLinked to original sources

PubMed1999

[Comorbidity of infantile autism and blindness].

A three year-old girl with Leber's amaurosis was found to fulfil the criteria for autism, as will as being mentally retarded. Despite her blindness she displayed unusually by early motor development and was able to move around in her surroundings without difficulty. This case suggests that this ability was due to her relevant use of a minor visual remnant and, as is often seen in blindness, a capability of reflecting the surroundings--a "bat sense". The pervasive development a disorder influenced her abilities more strongly than her sensory handicap.

Autistic Disorder↗

PubMed1992

Synthesis of a heteroglycuronan derivative containing the beta-D-galactopyranosyluronic acid (1-->3)-L-rhamnose repeating unit.

Helferich glycosylation of the cyanoethylidene L-rhamnose derivative 3 with the galactosyluronic bromide 2 gave the disaccharide 4 as a key intermediate in the synthesis of the monomer 13 for trityl-cyanoethylidene condensation (TCC). The following formation of the monomer 13, including introduction of a trityl group at O-3', proceeded in six steps. Because of the difficulty of some steps, an alternative route for 13 was tested. Model compounds 20, 21, and 22 were synthesized in order to confirm the stereoregularity of the products of the polycondensation. The polycondensation of the monomer gave D-GalpA-(1-->3)-L-Rha-oligomer derivatives consisting mainly of three repeating units. This result is in contrast with the degree of polymerisation (dp > or = 22) of other synthetic rhamnans, but is very similar to dp 2-7 of homo- and hetero-glucuronan derivatives.

Carbohydrate Sequence↗

PubMed1990

Scanning electron microscope observations on collagen fibers in human dentin and pulp.

Human permanent teeth were examined in the scanning electron microscope after demineralization and exposure to preparative procedures based on hydrogen peroxide, trypsin, and EDTA. These substances removed the inorganic material, the cellular structures, the homogeneous connective tissue ground substance, and interfibrillar matrix. The remaining tissue components comprised a network of distinct collagen fibers whose organization was related to the type of tissue in which these were incorporated. A similar or identical method has not been developed or applied to teeth previously. Dentin and predentin comprised a compact mass of fibers which basically were parallel to the continuously growing interior surface of the predentin, or arranged at an acute angle to this plane. Collagen fibers in the pulp were numerous, but lacked any particular orientation in most areas. Interodontoblastic fibers crossed the odontoblastic zone at a right angle to the pulp chamber wall and mingled with collagen fibers in predentin. When previously published findings of ours are taken into account, it is possible to conclude that other factors than the organization of the collagen fibers are responsible for the stainability of these fibers in predentin and in interglobular dentin with silver methenamine, and that aldehyde groups on collagen fibers in predentin may be actively and directly involved in the mineralization of the dentin.

Collagen↗

PubMed1990

High resolution scanning electron microscopy of the subplasmalemmal cytoskeleton in human odontoblasts.

The subplasmalemmal cytoskeleton in human odontoblasts was studied by high resolution scanning electron microscopy. The odontoblast layer was isolated and exposed to formaldehyde, glutaraldehyde, and OsO4 for some specimens, while the membraneous structures and soluble proteins in the dental tissue were removed by Zenker's solution and 1% OsO4 for other specimens, without further fixation of the remaining components. The cytoskeletal elements comprised a dense network of interlacing filaments of different diameters in the cell body. Most cytoskeletal elements were parallel to the axis of the cell processes situated inside the dentinal tubules. The appearance and orientation of the investigated subplasmalemmal cytoskeletal elements was unaffected by the choice of method. Both methods confirm the presence of a subplasmalemmal cytoskeleton in human odontoblasts.

Actin Cytoskeleton↗

PubMed1987

[Preparation of leukocyte-poor erythrocyte concentrates by glycerin treatment and washing].

The discontinuous washing procedure of red cell concentrates with saline was modified in order to improve the removal of leucocytes and platelets. It was achieved by glycerolization and deglycerolization without freezing and thawing. The elimination rate of leucocytes amounted to 99.9% and of platelets to 97.4% related to whole blood. A unit of red cell concentrate prepared from 400 ml of donated blood contained a total of (0.001 +/- 0.003) X 10(9) leucocytes and (2.35 +/- 1.33) X 10(9) platelets. The relatively high elimination rates are comparable with those obtained by filtration and cryopreservation, respectively. The whole procedure performed in glass bottles required one hour more than washing three times without glycerolization. A further improvement of the procedure should be directed towards saving time in processing and towards lowering the loss of red cells that is still 20%.

Blood Platelets↗

PubMed1978

Low cost internegatives from electron micrographs of metal shadowed objects.

Some observers prefer a contrast reversal of electron micrographs of metal shadowed objects, so that the metal-free shadows appear dark on the print. There are several ways of obtaining contrast reversal, and the reasons for using 35 mm internegatives are given and a setup for re-photography of electron microscopy films is described together with a modified enlarger. Both are equipped with nearpoint illumination, which can be diffused in a simple manner with minimal loss of intensity. Other features are a particularly rigid camera stand combined with a numbering device, and a glass- and scratch-free negative holder for the enlarger. The choice of lens for the re-photography is discussed.

Chytridiomycota↗

PubMedDate not supplied

Parvovirus B19 infections in patients with chronic anemia.

BACKGROUND AND OBJECTIVE: Parvovirus B19 has a marked tropism for erythroid progenitor cells and this may lead to chronic anemia in predisposed individuals. It was the purpose of the present study to investigate prospectively the frequency of parvovirus B19 infections in patients with a diagnosis of chronic anemia. METHODS AND RESULTS: Evidence of parvovirus B19 infection was found in 13/43 (30%) patients by demonstrating viral DNA and/or specific IgM antibodies through the use of PCR and ELISA techniques. Parvovirus B19 infection was established in 4 of 7 patients with hemolytic anemia, in 2 of 3 patients with pure red cell aplasia, in 2 of 9 patients with myelodysplastic syndrome, and in 2 of 10 patients with aplastic anemia. In 8 of the 13 positive patients only parvovirus B19 DNA could be detected, while 4 patients tested positive for both parvovirus B19 DNA and specific IgM. In the remaining positive patient only specific IgM could be detected. CONCLUSIONS: Since no predictive paraclinical or clinical features were observed we recommend that all cases of chronic anemia be tested for the presence of parvovirus B19 infection. Due to the discrepancies between DNA and IgM results, the diagnostic procedures should include a search for specific DNA by PCR methods if specific IgM has been found to be negative.

Adult↗