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Biomedical subjects

H Brantner

Publications and source records attributed to H Brantner.

At least 19 recordsLinked to original sources
PubMed1994

Applicability of diffusion methods required by the pharmacopoeias for testing antibacterial activity of natural compounds.

The diffusion methods required by various pharmacopoeias were investigated in comparison to the bioautographic TLC assay in regard to the applicability and the statistical evaluation for testing the antibacterial activity of natural substances. Naringenin as an example for a weakly effective lipophilic natural compound and tetracycline as standard were used. Data were analysed by linear regression and ANOVA. The comparison of the disk diffusion method, the hole-plate diffusion method, and the bioautographic TLC assay showed that nature and amount of substance influence to a great extent the selection of the test method. The evaluation of parallelism demanded by the pharmacopoeias may provoke problems in comparing natural substances with strongly effective antibiotics. The simultaneous influence of different variables was examined with the help of ANOVA. The bioautographic TLC assay showed for spotted compounds a comparable reproducibility and accuracy to the diffusion methods mentioned above.

Analysis of Variance↗
PubMed1980

A theoretical models of oncolysis by Clostridium oncolyticum M 55 ATCC 13,732.

Based on experimental data obtained by own experiments and investigations of other authors the first time it is tried to develop a new theoretical model of oncolysis by Clostridium oncolyticum M 55. The correlations between tumour, clostridial cells, the tumour kinin system and the immune system of the host are represented in their influence on oncolysis. The consequences for a therapeutic application of the tumour clostridia phenomenon are discussed.

Animals↗
PubMed1979

[Enzymological examinations on Clostrium oncolyticum M 55 ATCC 13,732 (author's transl)].

Since excenzymes are mainly involved in oncolysis by Cl. oncolyticum M 55 ATCC 13,732 the enzyme production of the strain should be examined under different growth conditions. The excretion of a protease and of two nucleases was observed in the nutrient medium for 72 h and the results obtained in a nitrogen atmosphere were compared with those obtained in an oxygen atmosphere. The cleavage of protein and of peptides was investigated by means of TLC. The importance of the exoenzymes for the oncolytic effect of Cl. oncolyticum M 55 was confirmed by animal experiments.

Clostridium↗
PubMed1978

[Investigations on the mercury pollutions of drinking water in the urban and rural regions of Styria (Austria) (author's transl)].

The paper reports on the inspection of more than 1000 drinking water supply plants with the purpose of determining the content of mercury. The investigations were carried out during the years from 1974 to 1977, and were intended to clarify whether in Styria (Austria), a partly industrialised and partly agricultural area, the ground water is polluted by mercury resulting from the use of mercury-containing plant preservation chemicals, seed dressings and industrial chemicals. The standard of assessment was taken from the German Drinking Water Regulation of 1975 which establishes a max. permissible mercury content of 4 ppb Hg for drinking water. In 70 per cent of the samples no mercury was detected. Of the remaining 30 per cent of the samples, about 90 per cent proved to have mercury below 1 ppb. The remaining water samples contained more than 4 ppb Hg and these values were attributable to geological or civilisatory causes. In two instances it was impossible to determine the origin of the mercury.

Austria↗
PubMed1975

The kininase activity of Ehrlichs' ascites solid tumor after treatment with oncolytic clostridia.

For the first time the effect of bacterial kininases of Cl. oncolyticum M 55 ATCC 13.732 could be demonstrated in the tumor itself. This is shown by an increased degradation of kinins by spore-treated tumor tissue in comparison with respective controls. The results indicate the influence of kininases on the capillary circulation of the tumor tissue as one of the fundamental reactions of oncolysis.

Animals↗
PubMed1975

[Studies on the culture of clostridium butyricum strain M 55 ATCC 13.732. 4th Communication: The influence of peptone quality upon kininase activity (author's transl)].

Studies of the oncolytic effect of Cl. butyricum strain M 55 resulted in the hypothesis of oncolysis to be an effect of a continuous production of kinin-decomposing enzymes. A decomposition of bradykinin will reduce capillary permeability and microcirculation in the tumour tissue, thus releasing and maintaining the process of necrotization. To achieve an optimal oncolytic effect of Cl. butyricum M 55, in addition to conditions already demanded in preceding papers, also an abundant production of kininase is required. Experience has shown peptone-containing media to be most suitable for culturing the M 55 strain, kininase formation being dependent upon peptone quality. The best yield of kinine-decomposing enzymes was achieved with the following peptones: peptone from Gelatine Merck, Tryptose Merck, Microbiotone Oxoid, Proteose Oxoid and Trypticase BBL. To obtain the greatest possible kininase production by the spores of Cl. butyricum M 55 applied for oncolytic therapy, the peptones mentioned are recommended for culture.

Bradykinin↗
PubMed1975

Studies on the cultivation of Clostridium oncolyticum M 55. 5th communication: The influence of iron, zinc, and cobaltous ions on the growth and the kininase activity of clostridium oncolyticum M 55 ATCC 13.732.

The precipitation or iron sulfide by hydrogen sulfide excreting Clostridium oncolyticum M 55 led to further examinations in order to avoid this disturbing factor. The method should render the cultivation of Cl. oncolyticum M 55 under definite conditions and should also intensify the formation and the enrichment of the clostridial kininases. Cobaltous ions and zinc granules were found useful for cultivation, because no disturbances increased from both during sterilization and incubation. The determination of the growth cycles and the replication rates showed the usefulness of zinc for substitution of iron. Cobaltous ions did only allow a low replication rate. These results confirmed those showing the influence of chelate-forming agents on the kininase activity. Cobaltous ions were not able to exchange the central atom of the metallo-proteide. The influence of the peptone quality on the kininase activity in presence of zinc was also examined, since examinations with iron media have shown a direct connection between peptone quality and enzyme activity. The results with zinc-containing media were nearly 5-10% lower than those with iron. The results allowed also the conclusion that the kininase excreted by Cl. oncolyticum M 55 is an iron-containing metallo-proteide, whose central atom is exchangeable for zinc. The consequences of the loss of enzyme activity for the oncolytic therapy with Cl. oncolyticum M 55 will be shown in further examinations.

Acetates↗