PubMed Health⌕ Search

Biomedical subjects

H Braunstein

Publications and source records attributed to H Braunstein.

At least 37 records · Page 2Linked to original sources

Congenital syphilis in aborted second trimester fetus: diagnosis by histological study.

A case of congenital syphilis in an aborted fetus in the second trimester of pregnancy was documented by demonstration of spirochaetes in the fetal liver, despite the fact that diagnostic studies had not been performed on the mother. Suspicion was aroused by the gross and microscopic appearance of the placenta, which appeared identical with the described in proven cases of congenital syphilis. Although these changes have been alleged to be non-specific, the rarity with which such well-developed lesions are encountered in placentas in other circumstances dictates the necessity for further study when they are observed.

Abortion, Spontaneous↗

Indole-positive strains of Klebsiella pneumoniae producing hydrogen sulfide in iron-agar slants.

The biochemical characteristics of six strains of Klebsiella pneumoniae that produced H2S in TSI slants are described. All were biotypical except for the consistent characteristic of indole production. It is suggested that the qualities of H2S and indole production are linked biochemical features acquired by episomal transfer. The attention of bacteriology laboratory workers is directed to these strains, which are easily distinguished biochemically from other H2S-producing organisms by their otherwise biotypical pattern.

Agar↗

A quantitative study of the multiplication of Pseudomonas aeruginosa in vented and unvented blood-culture bottles.

A quantitative study of the growth of Pseudomonas aeruginosa in both vented and unvented vaccum blood-culture bottles revealed that these organisms multiply readily in the latter. After a 3- to 4-hour lag period they double at 33--34-minute intervals, reaching a maximum growth of 10(8) - 10(10) organisms by 18 hours. Multiplication then stops. The data indicate that blind sampling should invariably be successful in isolating organisms after 8 hours of incubation. The rate of recovery of these organisms is not increased by venting, but the maximum level of growth in unvented containers is at the borderline level of visibility, necessitating a blind subculture for definitive diagnosis, whereas vented containers show relatively unrestrained growth. A method for the use of single vacuum bottles for the detection of Ps. aeruginosa, and optionally, Candida species, is proposed.

Aerobiosis↗

A quantitative study of the growth of Candida albicans in vented and unvented blood-culture bottles.

Suspensions of four strains of Candida albicans of various concentrations were inoculated in equal amounts into paired vented and unvented vacuum blood-culture bottles. Inocula ranged from 10 to 10(7) organisms. After 48 hours, it was found that unvented bottles contained essentially the same numbers of organisms as the original inocula, whereas heavy growth was observed in vented bottles. When the previously unvented bottles were subsequently exposed to room atmospheric pressure and incubated, all, even those with the smallest inocula, showed heavy growth in two days. It is concluded that Candida yeasts do not multiply under the microaerophilic conditions in such containers, but survive with the capability of subsequent proliferation. To avoid the necessity of employing two bottles, it is suggested that Candida be sought when indicated by venting the container at 48 hours after anaerobic subculture and again examining and subculturing for Candida two days later.

Aerobiosis↗