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H Brockerhoff

Publications and source records attributed to H Brockerhoff.

69 records · Page 4Linked to original sources
PubMed1967

Stereospecific analysis of triglycerides: an alternative method.

A new method for the stereospecific analysis of triglycerides is demonstrated on a corn oil which had been analyzed by an earlier method. The triglyceride (1,2,3-triacyl L-glycerol) was partially degraded by means of methyl magnesium bromide. The 1,3-diglyceride was then isolated, and converted into l-2-phosphatidyl phenol, from which phospholipase A cleaved the fatty acids from position 1. The remaining lysophosphatide was analyzed to yield the fatty acids at position 3 of the original triglyceride. The fatty acid composition of position 2 was determined after hydrolysis of the corn oil with pancreatic lipase. This method has the advantage over the one reported previously of yielding direct analyses of the fatty acids in each of the three positions.

Chromatography, Thin Layer↗
PubMed1967

Positional distribution of isomers of monoenoic fatty acids in animal glycerolipids.

The distributions of the following monoenoic acids were determined [notation: (position of double bond)-(chain length): (no. of double bonds)]: 7-, 9-, and 11-16:1; 7-, 9-, 11-, and 13-18:1; 9-, 11-, and 13-20:1; 9 + 11-22:1 and 13-22:1. As a rule, all isomers of a group show different distribution patterns. In the phospholipids of fish and mammals, the 7- and 13-isomers of 18:1 accumulate in position 1. In triglycerides of mammals fed on fish they accumulate in positions 1 plus 3, and this distribution is shared by 7-16:1 and 11-16:1 and by the groups 20:1 and 22:1. The positional distribution of the acids seems to depend on their structure, the 9-isomers in general accumulating in position 2; but in triglycerides, at least, the origin of the acid also seems to play a directing role, the exogenous acids being incorporated into positions 1 and 3. The variability of the distribution patterns of 9-16:1, 9-18:1, and 11:18:1, which contrasts with the regularity of the patterns for saturated and polyenoic acids, may be connected with the ability of the endogenous monoenoic acids to balance fluctuations in the supply of the exogenous polyenoic acids, and with the role of the fatty acid 9,10-dehydrogenation mechanism in the maintenance of structural and physical properties of phospholipids and triglycerides.

Adipose Tissue↗
PubMed1966

Stereospecific analyses of several vegetable fats.

The distribution of fatty acids among the positions 1, 2, and 3 of triglycerides of seven vegetable fats has been determined. The fatty acid compositions in positions 1 and 3 are very similar in most cases, but in none of the fats is the distribution completely symmetrical.

Dietary Fats↗