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Biomedical subjects

H Brunnemann

Publications and source records attributed to H Brunnemann.

At least 19 recordsLinked to original sources
PubMed1992

Clamydial antibody in sera of patients with trachoma.

Serum samples were taken in 1987 from 118 patients with trachoma seen in the Department of Opthalmology of the Gonder College of Medical Sciences, Gonder, Ethiopia, patients being staged according to MacCallan (II, III, IV), and classified in categories of different degrees of inflammation according to Dawson et al. Sera were investigated with the microIF test, using Chlamydia trachomatis serotypes A, B, C and partially D as antigens. Healthy Ethiopian students (n = 14), patients with chlamydial urogenital infections (n = 100), salpingitis (n = 47), and D-seronegative individuals (n = 574) served as controls. Patients with trachoma with severe inflammation showed significantly higher chlamydial antibody titres (greater than or equal to 1:128) against serotype A (p less than 0.05), B (p less than 0.01), and C (p less than 0.01) than patients with trivial, mild or moderate inflammatory symptoms. There was little correlation between MacCallan stages and chlamydial antibody titres, probably due to persistence of antibodies. In the control group of Ethiopian students only single persons were found to react with C. trachomatis serotypes A, B, and C in high titres; the geometric mean varied from x = 1:17 to x = 1:25 and distinctly differed from trachoma patients (x = 1:65). The individuals of other control groups showed high antibody titres against serotype C only in single cases and low geometric mean values.

Adolescent↗
PubMed1990

Comparison of different methods for the detection of antibodies to Chlamydia trachomatis in human sera.

Different methods for the detection of IgG antibodies to Chlamydia trachomatis were compared as to their specificity, sensitivity and efficacy. A modified microimmunofluorescence test (MIF) was used as a reference method. Two commercial ELISA tests kits (Chlamyset-antibody EIA, Orion, Finland and Chlamydelisa, Whittacker M.A. Bioproducts, USA), the complement fixation test (CFT) and five self-made ELISA tests with CFT antigens were compared with the reference method and with each other. In comparison to the reference method, the Chlamyset was found to be the most useful method. Three of the ELISA tests with CFT antigens were statistically comparable with the Chlamyset and the CFT, but the two others showed statistically significant differences. The Chlamydelisa and the MIF showed great differences from all other methods. The Chlamydelisa was very sensitive but apparently unspecific. Possible reasons for the different results are discussed.

Antibodies, Bacterial↗
PubMed1990

[Experimental microbiological research on instrument and denture disinfection with a disinfectant spray for dental practice and the use of patients. 2. Virological studies; the evaluation summary].

Carrier experiments using Echo- and Influenza-viruses on denture basis resins and fragments of orthodontic appliances resulted in good and even very good virucidal effects of disinfectant sprays Desident and Fesia-sept. The application of the disinfectant as spray seem to be advantageous, but is not recommendable. The diverse causes of this conclusion are discussed and an alternative way is proposed.

Aerosols↗
PubMed1990

[Chlamydia infection in gynecology].

The frequency of chlamydial infections was investigated by means of the McCoy culture technique and a serological procedure using the microimmunofluorescence test in female outpatients showing suspect symptoms; in patients suffering from acute salpingitis, and in women with involuntary infertility. In 18.6% of 290 subjects of the first group, chlamydiae were isolated from cultures. Of 26 patients with acute salpingitis, Chlamydia trachomatis could be detected in 10. The high frequency of positive serological tests suggests chlamydial infection to constitute an important etiological factor in female infertility and extrauterine pregnancies.

Adolescent↗
PubMed1989

[Genital chlamydia infections and extrauterine pregnancy].

Serum samples of 217 patients with ectopic pregnancy (e.p.) and of 152 women with intrauterine pregnancy (i.u.p.) were examined for their antibody content to chlamydia trachomatis by means of the indirect immunofluorescence technique. In 81% of the sera of patients with e.p., antibody could be detected, while patients with i.u.p. revealed antibody in 39%. Titres greater than or equal to 1:128 were found in 49% (e.p.) and in only 11% (i.u.p.) respectively; so they possess a high diagnostic value. These results show significant differences in the behaviour of antibodies in both groups of patients and are illustrated in 2 charts. Anamnestic data showed 54% of the patients with e.p. (i.e. adnexitis 23%, gonorrhea 12.3%, previous e.p. 18.4%); the mean age was 29 years. Affected tubes may lose their physiological function, possibly resulting in an ectopic pregnancy. The level of antibody in the serum remains stabile for many years, this enables a retrospective diagnosis of chlamydial infections. An early diagnosis and treatment, including the partners, represent the best way for prevention of complications.

Adult↗