Biomedical subjects

H Brustmann

Publications and source records attributed to H Brustmann.

5 recordsLinked to original sources

PubMed2000

Multilocular peritoneal inclusion cyst with extensive xanthogranulomatous stromal changes: a differential diagnosis of cystic pelvic tumors in women.

This report presents an unusual case of multilocular peritoneal inclusion cyst with extensive xanthogranulomatous changes in the cyst stroma occurring in a 21-year-old woman. The multicystic tumor was found attached to the mesentery of the terminal ileum. Microscopically, the cystic spaces were lined by flattened to cuboidal cells, which displayed immunoreactivity for cytokeratin but not for factor VIII-related antigen. Large stromal areas contained cholesterol clefts, multinucleated foreign body giant cells, and accumulations of foam cells. The pathogenesis of this multilocular peritoneal inclusion cyst remains obscure. The patient is disease-free 10 years after surgery. The most important differential diagnosis to this case is mature cystic teratoma of the ovary with xanthogranulomatous changes.

Adult↗

PubMed1999

Expression of nm23 in normal, hyperplastic and neoplastic endometrial tissues.

This study evaluated the expression of nm23 in curettage specimens from 63 cases of normal, hyperplastic and neoplastic endometrial tissues by immunohistochemistry. The histological diagnoses were as follows: normal proliferative (N = 5) or secretory (N = 5), simple hyperplasia (N = 11), complex hyperplasia (N = 9), atypical hyperplasia (N = 8) and adenocarcinoma (N = 25), consisting of endometrioid adenocarcinoma (N = 15), clear cell (N = 7) and serous papillary adenocarcinoma (N = 3). There was no immunostaining for nm23 protein in the 10 cases of normal endometria and in the 28 cases of endometrial hyperplasia. In contrast, 52% of the adenocarcinomas displayed a cytoplasmic staining pattern which was moderate to strong. This difference was statistically significant (p < 0.0001, chi-square test). nm23 expression in curettage specimens had no predictive value for determining the FIGO stage in the hysterectomy specimens (p = 0.2709, chi-square test). No significant difference for nm23 immunoreactivity was found between the histologic subtypes of endometrial adenocarcinoma (endometrioid versus serous papillary and clear cell, p = 0.1413, chi-square test). In this study, there was no immunostaining of normal endometria or of endometrial hyperplasia (including atypical endometrial hyperplasia) to support the hypothesis that expression of the nm23 gene product is related to the development of endometrial adenocarcinoma. In contrast, nm23 expression was upregulated in many cases of endometrial adenocarcinomas irrespective of the histologic subtype.

Adenocarcinoma↗

PubMed1997

The relevance of angiogenesis in benign and malignant epithelial tumors of the ovary: a quantitative histologic study.

The ability of a tumor to grow and eventually to infiltrate adjacent tissues requires a sufficient blood supply. Many malignant neoplasms have been shown to induce neovascularization. We asked whether it is possible to characterize ovarian epithelial tumors on the basis of vascularization and whether vascularization can be used to differentiate between benign and malignant neoplasms. We examined 14 cases of benign cystadenomas and 18 carcinomas. The microvessels were identified by immunohistochemical staining of endothelial cells for factor VIII. They were counted within the most vascular area of a tumor (neovascular "hot spot") on a x 100 and a x 400 field. Mean vessel counts were 51.64 [standard error of the mean (SEM), 5.7] in the benign cystadenomas and 131.05 (SEM, 6.7) in the group of carcinomas at a x 100 magnification. The mean microvessel counts per x 400 field were 14.4 (SEM, 1.9) in the benign and 33.7 (SEM, 3.45) in the malignant tumors investigated. These differences were significant (t test for independent samples, P < 0.001). Since our study shows significantly fewer small blood vessels in the benign cystadenomas than in the malignant tumors, the histologically determinable vascular density may be the basis for imaging blood flow by means of color ultrasound.

Adult↗

PubMed1995

Nucleolar organizer regions as markers of endometrial proliferation: a study of normal, hyperplastic, and neoplastic tissue.

Nucleolar organizer regions (NORs) are loops of DNA that transcribe to ribosomal RNA. They can be visualized as intranuclear black dots by histochemical staining with a colloid silver solution. We applied this method to 78 sections of endometrial tissue obtained either from curettage or from hysterectomy specimens. The histological diagnoses were as follows: normal proliferative (N = 9) or secretory (N = 5) endometrium, simple hyperplasia (N = 10), complex hyperplasia (N = 18), atypical hyperplasia (N = 8), and adenocarcinoma (N = 28). Mean silver-stained NOR (AgNOR) counts per cell were 3.2 (standard error of the mean [SEM], 0.2) in normal proliferative and 2.7 (SEM, 0.2) in normal secretory epithelium, and increased to 4.1 (SEM, 0.3) in simple hyperplasia, to 5.4 (SEM, 0.4) in complex hyperplasia, to 8.1 (SEM, 0.7) in atypical hyperplasia, and finally to 10.0 (SEM, 0.5) in endometrial carcinoma. The differences were significant (one-factor analysis of variance [ANOVA], P < .001). A slight increase but no significant difference was seen between the mean AgNOR counts in endometrial carcinomas of different histological grades. Our study suggests that AgNOR counts are reliable markers of endometrial proliferation and allow a clear distinction between benign, premalignant, and malignant epithelial changes. Our AgNOR findings in endometrial hyperplasia support the concept of various degrees of hyperplasia that can be differentiated on morphological grounds.

Adenocarcinoma↗

PubMed1994

[Histochemical characterization of endometrial proliferation using AgNOR (nucleolus organizer region)].

Nucleolar organizer regions (NORs) are loops of DNA that transcribe to ribosomal RNA. We used histochemical staining of NORs for 78 sections of endometrial tissue, obtained either from curettage or from hysterectomy specimens (normal endometrium 14, simple hyperplasia 10, complex hyperplasia 18, atypical hyperplasia 8 and adenocarcinoma 28). There was a significant positive correlation between mean AgNOR counts and the degree of hyperplasia. In adenocarcinoma, no significant difference was found between AgNOR counts in tissues of different histologic grades. Our study suggests that AgNOR counts are reliable markers of endometrial proliferation and allow a clear distinction between benign, premalignant and malignant epithelial changes.

Adenocarcinoma↗