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Biomedical subjects

H C Hsieh

Publications and source records attributed to H C Hsieh.

At least 73 records · Page 4Linked to original sources
PubMed1984

[Immunodiagnosis of human clonorchiasis by a micro-enzyme-linked immunosorbent assay].

A micro-enzyme-linked immunosorbent assay (ELISA) utilizing antigen from lyophilized Clonorchis sinensis adult worms extracted with triethanolamine buffered saline (TBS), veronal buffered saline (VBS) or phosphate buffered saline (PBS) were used to test 66, 31 and 9 sera from clonorchiasis patients, uninfected normal humen and patients with other parasitic infections, respectively. Four different combinations of incubation time between serum with antigen and serum with conjugate at either 37 degrees C or room temperature were tried. The combination of 6 hours and 16 hours reaction time processed at room temperature with shaking was chosen in this study because it revealed no pseudo-negative and cross reactions with high sensitivity, specificity and predictive value. There were no statistic difference among mean optical densities in micro-ELISA reactions of 66 sera (in 800 X dilution) from clonorchiasis patients with TBS, VBS or PBS antigen, respectively, but results obtained from TBS antigen seemed better than other two antigens. Good correlation was observed between the antibody titer or ELISA readings of sera form clonorchiasis patients and their worm burden expressed as eggs per gram of feces (EPG).

Antibodies↗
PubMed1983

Humoral reactions in human endocarditis due to Streptococcus bovis: evidence for a common S bovis antigen.

Humoral reactions to native culture filtrates of Streptococcus bovis were studied in patients with endocarditis by means of two-dimensional crossed immunoelectrophoresis. Serum from patients with S bovis endocarditis produced eight different precipitin arcs. Lex antigen cross-reacted with sera from patients with viridans streptococcal endocarditis, and TA antigen cross-reacted with ribitol teichoic acid-positive sera from patients with Staphylococcus aureus bacteremia. One antigen, common (c), was immunoreactive with all 10 sera from S bovis-infected patients. Antibody to this antigen was not found in sera from 77 patients with gram-positive bacteremia or in 29 other control sera. Common (c) antigen was found in all strains of S bovis isolated from patients. Rabbits immunized with formalinized S bovis cells of human origin also produced antibody to common (c) antigen. Common (c) may be an important antigen of S bovis and may in fact define this organism serologically.

Aged↗
PubMed1982

Modification of an experimental mouse Candida infection by human dialyzable leukocyte extract.

Human dialyzable leukocyte extract (10(7) - 10(8) leukocyte equivalents, containing transfer factor) was administered intraperitoneally to CFW mice the day of and 2 days after intravenous infection with Candida albicans. Tissue Candida populations were determined immediately after and 2, 4, 7 and 14 days after infection. Kidney populations were significantly reduced on 27% of the days studied. Similar reduction in C. albicans census was obtained after injection of leukocyte extracts from donors skin test-positive to Candida antigens or donors negative to Candida antigens by skin test and migration inhibition analyses. There was no evidence of a dose-response relationship for leukocyte extract in the range 10(5) - 10(9) leukocyte equivalents. When mice were primed with C. albicans antigen 4 weeks prior to challenge the efficacy of leukocyte extracts was not augmented. There was no evidence that the infection-reducing effects were related to augmented polymorphonuclear leukocyte mobilization, increased mononuclear clearance of C. albicans, or to a direct toxic effect on C. albicans blastospores. These studies suggest that the reduction in Candida populations was non-specific and give further impetus to the use of the dialyzable leukocyte extracts as non-specific supplements to antibiotics in overwhelming or recalcitrant infections in man.

Animals↗
PubMed1975

Epidemiologic studies of eosinophilic meningitis in southern Taiwan.

A study of the epidemiologic characteristics of 125 cases of eosinophilic meningitis or meningoencephalitis, probably caused by Angiostrongylus cantonensis, which occurred in southern Taiwan in 1968 and 1969 revealed a close association of the disease with the rainy season. In contrast to findings in other geographic areas, most such cases in this study occurred among children. A higher attack rate was observed among aborigines than among descendants of mainland Chinese. Most patients had eaten the giant African snail, Achatina fulica, prior to their illness and this mollusc was commonly found infected with third-stage larvae of A. cantonensis. However, in almost all instances the snail was eaten after it had been thoroughly cooked and examination of cooked snail meat revealed only dead larvae. It is suspected that patients became infected by inadvertently ingesting A. cantonensis larvae liberated when the snails were prepared for consumption.

Adolescent↗
PubMed1972

Distribution of Necator americanus and Ancylostoma duodenale in Liberia.

In the first country-wide survey of hookworm infection in Liberia, diagnosis by test-tube filter-paper cultivation revealed a prevalence of more than 90%. This sensitive technique also permitted differential species determination. Necator americanus was found to occur in all regions and in a high proportion of the persons examined. Ancylostoma duodenale was mainly confined to a narrow coastal belt, and usually parasitized individuals harbouring N. americanus as well. The average number of eggs per gram of faeces (EPG) of infected individuals was lowest in the population of the hinterland. During the latter part of the studies a modified test-tube filter-paper cultivation technique was used in combination with dilution counting to obtain data on intensity of infection separately for the 2 species. For N. americanus there was little regional difference in the intensity of infection, but the EPG for A. duodenale was much higher in people living in the coastal area.

Adolescent↗
PubMed1969

Inhibition of protease production of various bacteria by ammonium salts: its effect on toxin production and virulence.

Production of protease by many bacteria was found to be inhibited by ammonium salts, and the enzyme production was more sensitive to the salts than was growth of the organisms. Inhibition of protease production by some pathogenic bacteria may result in the recognition of an exotoxin which otherwise would have been digested by the protease. In the case of Pseudomonas aeruginosa, qualitatively different toxicities could be demonstrated in the culture fluids, depending on the presence or absence of protease in such a fluid. The toxicity of the culture in the presence of a high titer of protease may be due primarily to the protease, whereas the toxicity exhibited in the absence of protease could be due to proteinacious exotoxin. Producers of high titers of protease tended to be less virulent in vivo than producers of low titers of the enzyme, which exert their toxicities by a separate exotoxin.

Aeromonas↗
PubMedDate not supplied

Correlation of hepatocyte HBsAg expression with virus replication and liver pathology.

To elucidate the biologic significance of hepatocyte HBsAg, its expression patterns were correlated with virus replication and liver pathology in 578 liver biopsies taken from chronic HBsAg carriers aged 1 to 80 years. Five major patterns of hepatocyte HBsAg were identified: homogeneous [intense and discrete, (Pattern A), faint and discrete, (Pattern B) and faint and grouped (Pattern C)]; globular or spotty (Pattern D), and marginal (Pattern E). Pattern A was always associated with viremia and also very frequently with membrane HBsAg expression, but rarely with active liver disease. It occurred most commonly in HBeAg-positive carrier children and young adults, reflecting an early immune tolerance phase with active virus replication. Pattern B was also usually associated with viremia, but very commonly associated with active disease (70%), reflecting active virus replication with enhanced immune response. Pattern E (marginal HBsAg), which was always in group distribution resembling a clonal expansion, predominated the HBeAg-negative phase and was associated with absence of viremia and occurred mostly in older adults with inactive bipolar disease spectrum (normal liver/mild disease or cirrhosis/hepatocellular carcinoma); this reflects a late phase of inactive virus replication or integration. Patterns C and D did not correlate well with viremia, but also tended to have inactive diseases as did Pattern E. These findings suggest that hepatocyte HBsAg expression is closely related to the natural course of chronic hepatitis B virus infection.

Adolescent↗