Relative dependence on intracellular cyclic adenosine-3',5'-monophosphate levels of catabolite repressible proteins in Escherichia coli.
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Bacterial Proteins↗
Biomedical subjects
H Cailla
Publications and source records attributed to H Cailla.
Succinyl derivatives of adenosine 3',5'-cyclic monophosphate: synthesis and purification.
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Adenine Nucleotides↗
Enzyme immunoassay of 2'-5'-oligoadenylates at the femtomole level.
We have developed a competition enzyme immunoassay (EIA) for 2'-5'-oligoadenylates [p kappa(A2'p5')nA; 0 less than or equal to kappa less than or equal to 3; 1 less than or equal to n] based on an anti-A2'p5' A monoclonal antibody coated onto 96-well polystyrene plates and A2'p5' A peroxidase as a marker. It permits measurement of 5'OH(A2'p5')nA as such and p kappa(A2'p5')nA after alkaline phosphatase hydrolysis, with a detection threshold of 5 X 10(-12) M. All 2'-5'-oligomers were assayed with similar sensitivity. ATP and adenosine did not interfere at concentrations up to 10(6)-fold higher than those of 2'-5'-oligoadenylates. Reproducibility, stability of reagents and correlation with the radioimmunoassay were good. As such, this EIA is a suitable tool for studying the 2-5A system, particularly, in clinical investigations: the initial velocity of 2-5A synthetase can be determined on 10,000 cells without purification and the level of 2'-5'-oligoadenylates can be assayed on less than 1 ml of blood.
Adenine Nucleotides↗
High concentrations of 2-5A, the interferon intracellular mediator, in the blood of children with acute viral infections.
We measured the concentration of 2-5A (2',5'-oligoadenylate), an intracellular mediator of the antiviral action of interferon, in the blood of children with acute viral and bacterial infectious diseases. 2-5A concentration was found to be elevated in several children with viral diseases. This elevation seemed transient and was not specific for viral infections. We provide arguments for the use of 2-5A as a marker of the evolution of diagnosed viral diseases.
Acute Disease↗