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Biomedical subjects

H Caillens

Publications and source records attributed to H Caillens.

26 records · Page 2Linked to original sources
PubMed1979

[Biological changes observed after infusion of modified gelatin in women who had cesarean sections].

We suggest to study some biologic parameters after perfusion by 1,000 ml modified gelatin to young women who have a caesarian operation. The level of gelatin is determined by previously proposed method. Gelatin is also revealed at electrophoresis. Concurrently, protein contents in sera and hematocrit are estimated. These investigations are also made at the same time for a reference population who has a ceasarian operation without perfusion of gelatin. The highest level of gelatin is reached at t(0) + 90 mn. Gelatin is not present in sera at t(0) + 6 hr. At electrophoresis the beta-globulins raise concurrently with the level of gelatin. The alpha-globulins decrease but that variation is small and later. Protein level decreases also in conjunction with hematocrit. Hematocrit value is about 30 p. cent. It seems to the authors that this value allows a better oxygen transmission to tissues. For reference population these biologic parameters are not modified.

Adult↗
PubMed1979

[Comparative study of digoxin determination by E.L.I.S.A. and radioimmunoassay techniques (author's transl)].

This study is based on a double blind interlaboratory comparison between radioimmunoassay and E.L.I.S.A. digoxin determination. The correlation between digoxin values found with these two methods is good (r = 0.96 for therapeutic and toxic ranges 1,0 ng/ml - 5,5 ng/ml). The results indicate good repeatability, reproducibility and precision. The determination by E.L.I.S.A. can be performed with sera or plasma. The presence of haemolysis makes no appreciable difference in results. No variation is observed when different kits are used from an identical lot. However digoxin gives an important cross reactivity with digitoxin in Enzymeimmunoassay. Therefore it is necessary to know exactly the digitalis glycoside before the determination in order to avoid significant error.

Digoxin↗
PubMed1979

[Estimation of serum lipids: method without extraction adapted to autoanalysis (author's transl)].

An entirely automatic method, without prior extraction, is described for the determination of total serum lipids by a nephelemetric method. The results are compared with those obtained by Canal's method. There is a good correlation between the two technics. With a similar concentration of lipid the respective quantities of cholesterol and triglyceride do not intervene in the flocculation. The respective influence of the bilirubin concentration, hemolysis, and the concentration and nature of the serum proteins is considered. This method should permit simultaneous autoanalysis of the three parameters of classical lipid investigation: total lipids, triglycerides and cholesterol.

Autoanalysis↗
PubMed1978

[Demonstration and semi-automatic assay of gelatin in serum].

We suggest a semi-automatic method for the determination of gelatin. The measured concentration of gelatin is not dependant for the amino-acid composition of that plasma substitute preparation. Plasmatic proteins are separated previously from gelatin by precipitation using trichloracetic acid (5 p. cent; vol/vol). Modified gelatin lower in molecular weight than proteins can be found in the supernatant. It is determined by Lowry method with an automatic continuous flow system. Standardization must be realized using gelatin's solution since the developped coloration intensity with proteins is stronger. At electrophoresis, gelatin migrates essentially towards beta-globulins. beta-globulins increased with the gelatin level in serum. Percentage of variation for beta globulins and other fractions must be determinated in function of the protein level before perfusion. So it is possible to study the percentage of variation of these electrophoretic fractions independantly of the altered protein level.

Autoanalysis↗
PubMed1978

[Bamifylline. Conditions of its interference in the estimation of urinary 17-oxosteroids. Method of estimation using Zimmermann's reaction (author's transl)].

Bamifylline in therapeutic dosage may markedly disturb the determination of urinary 17-oxosteroids by Zimmermann's reaction, although the conditions of extraction and formation of this chromogenic substance are not always optimal for the metabolites of bamifylline. It is easy to suspect this interference as the chromogenic substance is produced as soon as the reagents are introduced, contrary to 17-oxosteroids, and it presents a more bluish colour. It is possible to separate completely both types of metabolite by washing the chloroform extracts with acid solutions which form salts with bamifylline and thus extract it. From these acid solutions, bamifylline may be recovered and estimated Zimmermann's reaction which is, for this purpose, modified.

17-Ketosteroids↗