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Biomedical subjects

H Champsaur

Publications and source records attributed to H Champsaur.

31 records · Page 2Linked to original sources
PubMed1982

Cholera-like illness due to Aeromonas sobria.

A Thai woman from Bangkok was admitted to a hospital in Paris for a cholera-like illness. A culture of her "rice-water" diarrhea was negative for Vibrio cholerae and enterotoxigenic Escherichia coli but was positive for Aeromonas sobria. This strain produced enterotoxin, cytolysin, proteolysin, hemolysin, and a cell-rounding factor. Acute -and convalescent-phase sera showed an increase in neutralizing antibodies to enterotoxin, cytolysin, and hemolysin. The enterotoxin, which was labile at 100 C, induced an accumulation of fluid in the rabbit ileal loop model and was not neutralized by antiserum to cholera toxin. Suckling mouse assays and rabbit permeability skin tests were negative, and the Y1 mouse adrenal cell assay produced not true cytotonic effect. This report, the first of an infection due to A. sobria alone, provides evidence that A. sobria is an enteric pathogen of humans that can cause a toxin-mediated, life-threatening illness.

Aeromonas↗
PubMed1980

Hemagglutination inhibition, single radial hemolysis, and ELISA tests for the detection of IgG and IgM to rubella virus.

Hemagglutination inhibition (HI), single radial hemolysis (SRH) and enzyme-linked immuno sorbent assay (ELISA), performed with commercial antigen and reagents are described and were compared in the three distinct situations that require rubella antibody detection. Determination of immunity status was carried out on 156 sera. A degree of correlation greater than 0.9 was found when comparing the three methods. Analysis of a further 74 sera, from 31 primary infections and three congenital syndromes, was performed to compare the occurrence of the various classes of antibodies in the three tests: HI test and IgM-ELISA become positive the day after the rash, whereas SRH test is not positive before the sixth day. From our limited study bearing on a total of 230 sera, each test has a precise assignment. For the determination of immunity status, SRH is simpler, faster, and inexpensive; absence or evidence of past infection can be unequivocally obtained especially in cases of low (1:10, 1:20) residual immunity. In the serodiagnosis of a rubella rash, SRH alone, due to the delayed rise in antibody titers, will demonstrate a complete seroconversion with a first serum collected up to the fifth day of the eruption. In case of absence of an early serum, of primary infection in a pregnant woman, of a newborn with suspicion of congenital syndrome, the measurement of rubella specific IgM is best obtained with ELISA, a procedure less time-consuming than HI following centrifugal, chromatographic, or electrophoretic separation, and "light" (8 S) RF with SRH test is discussed. Interference of IgM Rheumatoid Factor (RF) with IgM ELISA and IgG RF with SRH test is discussed.

Acute Disease↗
PubMed1978

[Loss of steroidogenesis in a mouse adrenal cell line after simian adenovirus 7 (SA7) transformation].

After transformation by the simian adenovirus 7 (SA7), the Y-1 Mouse adrenal tumor cells are no longer able to produce steroïds either spontaneously, or after specific stimulation. Nevertheless, these stimulations are able to increase the level of cAMP both in transformed and original cell line. The differentiation is associated with an intracellular modification of steroïdogenesis which seems to be localized in the metabolic chain of steroïd synthesis after the increase of the cAMP level.

Adenoviridae↗
PubMed1978

[Use of hemolysis in gel in the serodiagnosis of rubella. Comparison with hemagglutination inhibition].

The hemolysis in gel test (HIG) was compared with the hemagglutination inhibition test (HI). This new test is simple and time-sparing since it does not require dilution or serum pre-treatment, and can be measured directly in mm. It is not more expensive than the HI method. It has proved sensitive and is not affected by non specific serum hemagglutination inhibitors and is conclusive whenever HI titers are looked upon with suspicion (titers of less than 1/20). It is therefore well adapted to mass screening of immunity against rubella but in the field of recent infections HI method still has its role to play. Using the HIG test as a reference, we measured the loss of Ig to rubella following the three most commonly employed methods for removal of inhibitors: the mean values of 16 sera were 25% of antibodies lost after kaolin pre-treatment, 62% lost after heparine-Mn-Cl2 procedure 75% lost after dextran-CaCl2 treatment.

Hemagglutination Inhibition Tests↗
PubMedDate not supplied

[Rotavirus gastro-enteritis in infants (author's transl)].

Deviation of complement antibodies were studied in 630 hospitalized infants aged from 0 to 4 years suffering from rotavirus gastro-enteritis, over a period of one year. Seroconversion was observed in 30 cases out of the 256 infants in whom 2 serological samples were taken. In 29 cases out of 30, these rotavirus infections occurred in infants under 18 months of age, and they were all seen during ther period from november to march, rotavirus being responsible for one third of the gastro-enteritis cases. A serological study according to age-group in 640 infants gave positive results in 75 per cent (1st semester), 68 per cent (2nd semester), 75 per cent (2nd year), 78 per cent (3rd and 4th years).

Age Factors↗