Biomedical subjects

H D Appleton

Publications and source records attributed to H D Appleton.

10 recordsLinked to original sources

PubMed1980

Creatinine: a review.

Measurement of creatinine has many applications. We review the determination of urinary creatinine as a valid index of completeness of 24-h urine collection, the clinical utility of the determination of creatinine clearance ratios, and measurement of the ratio of the clearance of specific analytes, such as amylase, to the ratio of clearance of creatinine. The chemistry and variables that affect the Jaffé reaction are reviewed, and attempts at improvement of specificity are discussed. We also review and assess techniques other than the Jaffé reaction for measurement of creatinine.

Amylases↗

PubMed1979

The biochemical and histochemical demonstration of lactase induction in fetal rat intestine by intra-amniotic injection of lactose.

A single dose of 20 mg beta-D-lactose injected into the amniotic sac of rats on day 17 of pregnancy induced an increase in lactase activity in fetal jejunum. This effect was first noted two days after injection and lasted for at least two additional days. Histoenzymatic investigation indicated that this enzyme was located on the surface of the absorptive cells lining the villi and thus corresponds to the "dietary" form of beta-galactosidase. A much smaller increase, based presumably on progressive increase in fetal size (age) was found in control fetuses which had received glucose or no injections. Peak lactase values in fetuses receiving lactose were substantially higher than peak values in control fetuses. In both lactose-injected and non-injected rats which were allowed to deliver, there was a sharp drop in lactase values coincident with birth.

Amnion↗

PubMed1972