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Biomedical subjects

H D Chen

Publications and source records attributed to H D Chen.

49 records · Page 3Linked to original sources
PubMed1986

Occurrence of donor Langerhans cells in mouse and rat chimeras and their replacement in skin grafts.

Evidence is presented that some endogenous Langerhans cells (LCs) may persist indefinitely in skin grafts. This evidence is based on the observation that although 2 weeks after grafting F1 hybrid mice and rats with genetically compatible skin, most of the LCs in the grafts were replaced with those of the host, some LCs of graft origin persisted for as long as the grafts were followed (154 days in mice and 249 days in rats). It has also been demonstrated that the spleen may be as good a source of LCs as the marrow. Thus, 6 weeks after lethally irradiated mice were restored with F1 hybrid spleen cells, most of the LCs in the epidermis of their pinnae were of donor origin. LCs of donor origin also were found in the epidermis of the pinnae of animals that had been inoculated at birth with spleen and lymph node cells (mice) or bone marrow cells (rats). Hence the occurrence of these cells provides another means of confirming that tolerance (chimerism) has been induced.

Animals↗
PubMed1985

Influence of major-histocompatibility-complex-compatible and incompatible Langerhans cells on the survival of H-Y-incompatible skin grafts in rats.

BN male trunk skin grafts, the Langerhans cells (LC) of which have been replaced with major histocompatibility complex (MHC)-incompatible Lewis female cells, survive longer on BN female rats than BN male trunk skin grafts bearing BN female LC. Evidence is presented that the privilege afforded these grafts is a consequence of MHC restriction. Thus, supplanting the native LC population of BN male trunk skin grafts with MHC-compatible Lewis.1N female cells has no affect on their survival on BN female rats. Evidence is also presented that H-Y-incompatible isografts deficient in MHC-compatible LC induce tolerance of H-Y.

Animals↗
PubMed1984

Major histocompatibility complex restriction and transplantation immunity. A possible solution to the allograft problem.

Studies of the survival of weakly histoincompatible skin grafts in which the Langerhans' cells (LC)5 have been replaced with major histocompatibility complex (MHC)-compatible or incompatible LC, as well as studies of the fate of cultured endocrine allografts in MHC-compatible and incompatible mice and rats, indicate that allografts are only recognized as foreign if they possess donor macrophages (or cells of this family), or if antigen presenting cells MHC-compatible with the graft can be provided by the host.

Animals↗
PubMed1983

Influence of Langerhans cells on the survival of H-Y incompatible skin grafts in rats.

Evidence is presented that Langerhans cells (LCs) play an important role in the rejection of H-Y incompatible skin grafts in BN rats. Not only are small male trunk skin isografts much more likely to be rejected by BN females than similarly sized male foot pad, tail, and ear skin transplants, all of which possess fewer LCs than trunk skin, but the survival of male trunk skin grafts can be prolonged by replacing their LC population with allogeneic female cells.

Animals↗
PubMed1982

Studies on the behavior of H-Y incompatible skin grafts in rats.

Studies on the behavior of H-Y incompatible skin grafts in rats indicate that 1) there is considerable variation in the ability of females of different strains to reject male skin isografts; 2) small, male trunk skin isografts are more likely to be rejected than similarly sized ear or tail skin grafts; 3) other factors besides the MHC plan an important role in determining the ability of females to reject male skin isografts; 4) the ability of adult male skin grafts to induce unresponsiveness to H-Y depends upon their size (large grafts are more effective than small) and the genotype of the host; 5) even in those strains in which females usually accept small, male trunk skin isografts, such grafts are acutely rejected by sensitized females; and 6) the total amount of H-Y incompatible trunk skin the host is exposed to, rather than the size of each graft, determines their fate.

Animals↗
PubMedDate not supplied

Characterization of latex allergenic components by capillary zone electrophoresis and N-terminal sequence analysis.

In a previous study, protein components purified from latex gloves that elicited allergenic reactions were characterized by sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), and yielded apparent molecular weights of 14, 22, 30, 34, 46, and 58 kD. These allergenic components were isolated for further characterization by capillary zone electrophoresis and N-terminal amino acid sequence analysis. These components all migrated at approximately 25 and 35 min on capillary zone electrophoresis. Diode array spectral analysis detected indistinguishable characteristics between these two protein peaks. In addition, complex formation of these components with patients' immunoglobulin was demonstrated by capillary zone electrophoresis. Analysis of components separated by SDS-PAGE on a polyvinylidene difluoride membrane showed that the first 13 residues were identical to the sequence of hevein. Based on the criteria of charge-to-mass ratio and N-terminal amino acid sequence, our results suggest that these components of latex proteins are similar in the primary structure.

Allergens↗
PubMedDate not supplied

Complete ophthalmoplegia in a hypertensive diabetic patient.

Combined paresis of all three ocular motor nerves is a rare ocular manifestation in diabetic patients. We present a case of a 73-year-old female with complete ophthalmoplegia. There was no evidence of peripheral neuropathy. Diagnostic angiography showed atherosclerotic stenosis in the cavernous segment of left internal carotid artery. The ophthalmoplegia had a self-limited course despite the severe vascular lesions.

Aged↗
PubMedDate not supplied

Characterization of latex allergens and correlation of serum IgE/IgG antibody ratio with clinical symptoms.

Enzyme-linked immunosorbent assay (ELISA) was used to study latex-specific IgE and IgG. Levels of these immunoglobulins were correlated with latex allergy severity. Total protein content and relative immunoreactivity of 12 different brands of disposable gloves were compared. Specific latex proteins were extracted and characterized by immunoblotting. Blots revealed that the major latex allergen, reactive with both IgE and IgG, was a 34 KD component. Protein content varied among brands of gloves but correlated with immunoreactivity (R = 0.683; P < 0.017). The ratio of IgE/IgG correlated positively with symptom severity (R = 0.689; P < 0.001) and was useful to predict the severity of latex allergy.

Adult↗