Biomedical subjects
Publications and source records attributed to H D Krause.
A reduction in the motility of the spermatozoa in stallion semen stored in pellet form for 23 years in liquid nitrogen at -196 degrees C could not be seen after thawing. The insemination of a mare with this semen resulted in a normal pregnancy. A normally developed, healthy male foal was born after a gestational period of 321 days.
Explore the source record for details and available documents.
In order to establish normal values, samples of cerebrospinal fluid from 109 healthy children between the ages of 0 and 13 years were analyzed for total protein, different protein fractions after electrophoretic separation, and IgG. Total protein was determined by the biuret method after precipitation with trichloroacetic acid. For the determination of the different protein fractions, the fluid was concentrated by high pressure filtration; the fractions were separated by microzone elektrophoresis on cellulose acetate membranes, then assayed photometrically after staining with amido black B. IgG was determined by radial immunodiffusion.
This paper summarizes a survey of the blood typing capabilities of representative American laboratories as they are: 1) currently applied to disputed paternity cases and 2) these laboratories potential for utilizing additional tests in disputed paternity cases.