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H D MAYOR

Publications and source records attributed to H D MAYOR.

At least 19 recordsLinked to original sources
PubMed1965

REOVIRUSES. II. STRUCTURE AND COMPOSITION OF THE VIRION.

Mayor, Heather D. (Baylor University College of Medicine, Houston, Tex.), Richard M. Jamison, Liane E. Jordan, and M. Van Mitchell. Reoviruses. II. Structure and composition of the virion. J. Bacteriol. 89:1548-1556. 1965.-Reovirus type 1 has been grown in green monkey kidney cells and harvested 24 hr after infection ("early" virus) and 96 hr after infection ("late" virus). A number of biological parameters have been determined on purified preparations of both "early" and "late" harvests of reovirus. There were no significant differences in values obtained for the molecular weight, RNA content, and buoyant density of virions prepared from early or late harvests. The size of the capsids and their morphology were also identical. Late harvests of reovirus were particularly rich in empty viral capsids (density, 1.28 in cesium chloride), and a significant number of empty inner capsid shells were routinely found. These shells could be prepared readily by controlled digestion of complete virus particles with trypsin. The inner shell appears to be composed of subunits packed with icosahedral symmetry to form a 45-mmu foundation on which the outer 92-subunit capsid is assembled. The inner shell is somewhat reminiscent in size and morphology of the capsid of papovaviruses. The fact that it can exist as a discrete entity has prompted us to propose some modifications to the current models for the reovirus capsid.

Animals↗
PubMed1964

A LABILE INTRANUCLEAR RNA ASSOCIATED WITH THE DEVELOPMENT OF ADENOVIRUSES.

The nucleic acids produced intracellularly during the replication cycles of both DNA and RNA viruses can now be identified rapidly using a sensitized procedure based on staining with the fluorochrome acridine orange. Cellular DNA, viral DNA (both single and double stranded forms), cellular RNA, and RNA arising as a result of viral stimulus can be differentiated. The intracellular development of virus specific DNA, RNA, and protein has been studied in monkey kidney cells infected with adenoviruses types 3 and 7. It has been possible to detect a labile RNA in the nucleus from 16 to 20 hours after inoculation. When the cultures are treated with puromycin at this time, this RNA can be accumulated under certain conditions in the nucleus and demonstrated cytochemically. At the same time the production of specific viral protein as determined by staining with fluorescein-labeled antibodies is markedly inhibited. However, intranuclear double stranded DNA continues to be formed for a time. When puromycin is added to the system early in the eclipse period virus-specific DNA and labile RNA cannot be detected.

Acridines↗
PubMed1962

Icosahedral models and viruses: a critical evaluation.

The evidence for a 42 capsomere structure for the capsid of the papova viruses and 92 for reovirus and woundtumor viruses is corroborated. Some of the difficulties inherent in comparing large-scale icosahedral models with high-resolution electron micrographs of virus particles displaying cubic symmetry are discussed.

Capsid↗