Biomedical subjects

H D Pulford

Publications and source records attributed to H D Pulford.

2 recordsLinked to original sources

PubMed1976

Plasma and organ counts of 3H in normal or immune mice and sheep injected with 3H-epsilon-dinitrophenyl-lysine.

Mice and sheep with circulating antibody to dinitrophenol (Dnp) retained injected 3H-epsilon-Dnp-lysine in their circulation for three and seven days respectively. In normal mice and sheep the 1 h post injection counts of 3H/g of kidney were higher than those per ml of plasma. In immune mice, plasma 3H counts were always higher than kidney counts, whereas at 1 h in immune sheep, plasma and kidney counts were similar. Liver, spleen and lung counts were also made in some experiments. Increased 3h counts could be demonstrated in mice treated passively with sheep anti-Dnp serum and then given 3h-epsilon-Dnp-lysine. The fast gamma-globulin of sheep antiserum is mainly responsible for retention of hapten in mouse plasma.

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PubMed1975

Antibody-hapten interactions in vivo in mice and sheep.

From 5 min to 5 h after an intravenous injection of one of the haptens, elipson-dinitrophenyl-lysine, 2,4-dinitrophenol (DNP), or procaine, mice that were actively immunised against these haptens held more of the hapten in their plasma than did normal mice. Over the same time interval, mice that had been passively immunised with sheep anti-procaine antisera and then treated with procaine held more procaine in their plasma than did mice treated with normal sheep serum. When procaine or DNP was administered orally or intraperitoneally to sheep with circulating antibody to the hapten, the antibody titre was usually reduced 1 h after dosing but returned to the pre-dosing titre by 24 h. Experiments indicated that the reduction in antibody titre was due to in vivo neutralisation of antibody by the hapten.

Animals↗