Biomedical subjects

H Danbara

Publications and source records attributed to H Danbara.

75 records · Page 5Linked to original sources

PubMed1975

Accumulation of gold in various organs of mice injected with gold thioglucose.

The response of either male or female mice to the administration of gold thioglucose (GTG) was observed in food intake, in body and organ weights, and in gold accumulation in respective organs. Male mice were more sensitive to GTG toxicity than females. During 103 days after GTG administration, the adipose tissues of the females and males increased in weight 15 times and 2.5 times as high as those of control mice, respectively. The weights of heart, lung, and brain were not affected by GTG administration. In the respective organs, gold concentrations, which were determined by neutron activation analysis, were high in liver, kidney, spleen, adrenal, and adipose tissue (especially in female) within a shorter time after the GTG injection. In various organs of nonobese mice, the concentrations of GTG were significantly less than those in the typical obese mice. Even after more than 100 days after injection, gold was detected in the respective organs. In the control mice without any GTG administration, no gold could be detected.

Adipose Tissue↗

PubMed1975

Plasmid-determined epistatic susceptibility to kasugamycin.

The effect of two representative plasmids, R100 and F8-gal, on the susceptibility of Escherichia coli to kasugamycin was studied. R(+) and F(+) cells were found to be more susceptible to this antibiotic than R(-) and F(-) cells, respectively. Retransfer and curing experiments of these plasmids show that this increased susceptibility of host cells to kasugamycin was conferred by either of the plasmids. At the early stage of growth of R100(+) cells in the presence of kasugamycin, R(-) segregants overgrew the population and then they were replaced by kasugamycin-resistant mutants of the R(+) cells which became the majority cell line of the population. The former phenomenon is assumed to be due to the increased susceptibility of R100(+) cells to kasugamycin, and the latter is probably related to the finding that R100 enhances the spontaneous mutation of host cells to resistance to kasugamycin. The practical and experimental significance of these findings are discussed.

Aminoglycosides↗

PubMedDate not supplied

Regional and subcellular distribution of gold in brain of gold thioglucose obese mouse.

By employing neutron activation analysis, endogenous content of gold was estimated quantitatively in discrete brain areas and in subcellular fractions of the hypothalamus of gold thioglucose (GTG) induced obese mice. The highest concentration of gold was obtained in the ventromedial hypothalamus (VMH) reaching approximately 100 ng/mg wet tissue. Significantly higher concentrations were observed in other hypothalamic subareas followed by certain limbic areas and the thalamus, while in the cerebral and the cerebellar cortex the gold concentration was very low. Subcellularly, the hypothalamic gold was principally recovered in the supernatant fraction particularly after a hyposmotic shock treatment of the crude mitochondrial fraction. Contrary to GTG, treatment with gold thiomalate (GTM) did not induce obesity in the mouse, although considerable amount of gold was observed in the VMH, a finding suggesting the existence in the VMH of at least a two step mechanisms for inducing GTG obesity. To identify the satiety neuron transmitter, an analysis of certain enzyme activities involved in the synthesis of known transmitters, such as acetylcholine or gamma-aminobutyric acid (GABA), was made in the GTG-obese mice. There were no significant changes in any of the areas functionally related to the VMH.

Animals↗