PubMed Health⌕ Search

Biomedical subjects

H Dene

Publications and source records attributed to H Dene.

46 records · Page 3Linked to original sources
PubMed1983

The phylogenetic position of aardvark (Orycteropus afer) as suggested by its myoglobin.

Skeletal muscle myoglobin of the aardvark (Orycteropus afer) was isolated and its primary structure determined. The amino-acid sequence was then used in conjunction with previously established myoglobin sequences to evaluate the phylogenetic relationships of the aardvark. The most parsimonious trees constructed from this myoglobin sequence data either alone or when combined with lens alpha-crystallin A sequence data depict the aardvark lineage as one of the most ancient among Eutheria.

Amino Acid Sequence↗
PubMed1982

Ochotona princeps (pika) myoglobin: an appraisal of lagomorph phylogeny.

Myoglobin was purified from skeletal muscle of the pika (Ochotona princeps) and its primary structure was determined. This sequence was added to the set of 64 already known vertebrate myoglobin sequences and used to evaluate the phylogenetic position of the pika among other mammals, using a computerized search procedure based on maximum parsimony criteria. The pika is clearly related to the rabbit (Oryctolagus cuniculus), with which it is traditionally associated as a member of the order Lagomorpha. A monophyletic group composed of Lagomorpha, Scandentia, and Carnivora is a consistent feature of the dendrograms produced. An association of Carnivora and Lagomorpha casts doubt on the results of those investigations using rabbit antisera in systematic studies and depending on carnivores as an outside reference group for cladogram construction.

Amino Acid Sequence↗
PubMed1981

An exceptional amino acid replacement on the distal side of the iron atom in proboscidean myoglobin.

Amino acid sequence determination of elephant myoglobin revealed the presence of the unusual substitution E7 His leads to Gln. Stereochemical analyses suggest that the most suitable residue which can functionally substitute for His at this position in vertebrate globins is Gln. Physiochemical studies imply that the slower rate of autooxidation of elephant myoglobin is the result of this substitution which may confer some selective advantage on the species. Comparative sequence data of paenungulate myoglobins suggest that the His leads to Gln mutation probably occurred in an ancestor of Elephantinae.

Amino Acid Sequence↗
PubMed1980

The myoglobin of primates X.

The amino acid sequences of skeletal muscle myoglobins from two old-world monkeys, Presbytis entellus and Erythrocebus patas, as well as one new-world monkey, Cebus apella wer inferred by homology of the tryptic and peptic peptides with the known sequence of human myoglobin and by selective dansyl-Edman degradation. These new sequences were examined phylogentically in conjunction with the 15 primate sequences already reported. It is clear that myoglobin evolution has been extremely conservative among cercopithecoid primates, so much so that the two surviving subfamilies cannot be distinguished using this protein.

Amino Acid Sequence↗
PubMed1980

The amino acid sequence of alligator (Alligator mississippiensis) myoglobin. Phylogenetic implications.

The amino acid sequence of myoglobin from cardiac muscle of the American alligator (Alligator mississippiensis) was established by alignment of overlapping peptides and dansyl-Edman degradation. The chain initiating methonine seems to be retained. Thus, this molecule has 154 amino acid residues rather than 153 as in other tetrapod myoglobins. Maximum parsimony analysis indicated that alligator myoglobin diverges more from both bird and mammal myoglobins than these latter do from each other. The phylogenetic implications are discussed with respect to a possible diphyletic origin for diapsid reptiles.

Alligators and Crocodiles↗
PubMed1980

The amino acid sequence of elephant (Elephas maximus) myoglobin and the phylogeny of Proboscidea.

The complete amino acid sequence of skeletal myoglobin from the Asian elephant (Elephas maximus) is reported. The functional significance of variations seen when this sequence is compared with that of sperm whale myoglobin is explored in the light of the crystallographic model available for the latter molecule. The phylogenetic implications of the elephant myoglobin amino acid sequence are evaluated by using the maximum parsimony technique. A similar analysis is also presented which incorporates all of the proteins sequenced from the elephant. These results are discussed with respect to current views on proboscidean phylogeny.

Amino Acid Sequence↗
PubMed1976

Immunodiffusion systematics of the primates. III. The strepsirhini.

Immunodiffusion comparisons have been run using 26 antisera including seven made to strepsirhine species. Spur size data resulting from these comparisons have been analyzed by computer and depict Strepsirhini as a monophyletic group within Primates including Lemuriformes and Lorisiformes. Cheirogaleidae is retained with Lemuriformes. Indriidae rather than Cheirogaleidae is depicted as closer to Lemur. Evidence is presented indicating that Lorisiformes is composed of three groups rather than two as in traditional classifications. The three groups, which are considered here to diverage at a family level, are Galagidae, Lorisidae, and Perodicticidae.

Animals↗
PubMedDate not supplied

Maternal effects on blood pressure and survivability in inbred Dahl salt-sensitive rats.

Maternal effects on blood pressure response to high salt diet were evaluated using inbred Dahl salt-sensitive (S/JR) and inbred Dahl salt-resistant (R/JR) rats. A cross-fostering experiment did not yield any evidence for a consistent effect of strain-specific fostering environments on the subsequent blood pressure response of S/JR or R/JR rats. A small increment in blood pressure seen only in male rats associated with the maternal R/JR environment was probably the result of effects mediated through an increment in body weight. In an experiment in which litter size was varied, weaning body weight was found to be an important predictor of survivability of S/JR rats fed a high salt (8% NaCl) diet; higher body weight was associated with longer survival in both males and females. Higher body weight at weaning also was associated with a small increment in blood pressure of S/JR rats in male, but not in female, rats. Intrauterine environment was evaluated using the embryo transplant technique. No evidence for a difference between S/JR and R/JR intrauterine environments with regard to blood pressure response of S/JR pups to salt was found. It is concluded that the early nutritional level during nursing as altered by varying litter size has important effects on body weight and survivability of S/JR rats on high salt diet, but that these effects are not mediated through changes in blood pressure. We found no evidence for genetic effects on blood pressure operating through the early maternal intrauterine or fostering environments.

Animals↗
PubMedDate not supplied

Development and characteristics of inbred strains of Dahl salt-sensitive and salt-resistant rats.

Several inbred lines of rats were produced from noninbred stock of Dahl salt-sensitive (S) rats, and several inbred lines were also produced from noninbred stock of Dahl salt-resistant (R) rats. There were significant differences (p less than 0.001) in blood pressure response to a high salt diet among the inbred S lines produced, which indicates that the original S stock obtained from Brookhaven Laboratories is not genetically homogeneous. There were no significant differences in blood pressure among the inbred R lines produced. One inbred strain of S and one inbred strain of R with the appropriate blood pressure responses were ultimately brother-sister mated for more than 20 generations. These inbred strains were called S/JR and R/JR respectively. Fulminant hypertension and marked vascular and renal lesions developed in the S/JR after 3 to 4 weeks on a high salt (8% NaCl) diet, and all S/JR were dead within 8 weeks on the high salt diet. In contrast, R/JR survived well on a high salt diet, and hypertension or vascular and renal lesions did not develop. Hypertension and associated vascular and renal lesions developed in S/JR on a low salt diet (0.4% NaCl), but this took 3 to 4 months. These characteristics are similar to those originally reported by Dahl for his noninbred, continuously selected stocks. The R/JR were found to have mild hydronephrosis at 4 months of age, which probably is genetically determined and which may have been fixed inadvertently in the strain during inbreeding.(ABSTRACT TRUNCATED AT 250 WORDS)

Administration, Oral↗