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H Dorfman

Publications and source records attributed to H Dorfman.

17 recordsLinked to original sources
PubMed2000

Recurrent chromosome aberrations in fibrous dysplasia of the bone: a report of the CHAMP study group. CHromosomes And MorPhology.

The nosologic status of fibrous dysplasia (FD), a well-known and relatively common bone lesion, is controversial. Information collected by the CHromosomes And MorPhology (CHAMP) study group on published and unpublished cases of fibrous dysplasia shows the presence of clonal chromosome changes in at least a proportion of these lesions. The chromosome aberrations found in FD lesions have been quite variable and have included both structural and numerical changes. Two of the three cases investigated at the study group had trisomy 2 as the sole acquired anomaly. Combined with previously published data, +2 and rearrangements involving chromosome band 12p13 have each been detected in 3 of 8 cases with abnormal karyotype of 11 in which chromosomal analysis has been performed, suggesting that FD is a neoplastic lesion rather than a "dysplastic" process, as has been generally believed and as implied by its very name.

Adolescent↗
PubMed2000

Cytogenetic-morphologic correlations in aneurysmal bone cyst, giant cell tumor of bone and combined lesions. A report from the CHAMP study group.

Aneurysmal bone cyst and giant cell tumor of bone are relatively rare bone tumors that sometimes coexist. We examined the karyotypes of 3 aneurysmal bone cysts, 12 giant cell tumors, and 3 combined lesions. All aneurysmal bone cysts showed involvement of chromosome segments 17p11-13 and/or 16q22. In addition, in 1 of the 3 giant cell tumors with secondary aneurysmal bone cyst, both chromosome bands were rearranged as well, albeit not in a balanced translocation. Seven out of 12 giant cell tumors were characterized by telomeric associations. One giant cell tumor showed a dup(16)(q13q22), suggesting the presence of a (minor) secondary aneurysmal bone cyst component, despite the absence of histological proof. Our results, combined with literature data further substantiate that segments 16q22 and 17p11-13 are nonrandomly involved in at least some aneurysmal bone cysts, irrespective of subtype (primary, secondary, intra/extraosseous, solid or classic). These findings strongly suggest that some aneurysmal bone cysts are true neoplasms. In addition, telomeric associations are the most frequent chromosomal aberrations in giant cell tumor of bone, the significance of which remains elusive. In combined giant cell tumor/aneurysmal bone cyst each component seems to retain its own karyotypic abnormality.

Adolescent↗
PubMed1995

Mesenchymal chondrosarcoma with secondary orbital invasion.

Orbital involvement with mesenchymal chondrosarcoma is rare. Until recently, despite the young age of the affected population, exenteration was the recommended management. We report a patient with orbital invasion by mesenchymal chondrosarcoma managed surgically without exenteration. Adjuvant chemotherapy and radiotherapy were administered. There is no evidence of local or distant tumor recurrence with 30 months of postoperative follow-up. This and other recent case reports suggest that exenteration may not be necessary for local tumor control of mesenchymal chondrosarcoma in the orbit.

Adolescent↗
PubMed1988

Radiologic features of well-differentiated osteosarcoma.

Eight cases of well-differentiated, intramedullary osteosarcoma were studied. In three cases, the lesions previously had been diagnosed, pathologically and radiologically, as benign. The distal femur was the most frequent site. Radiographically, the tumor margins were poorly defined in five cases and were well defined in three. In seven cases, the bone response pattern was both lytic and blastic; in one case, it was purely lytic. Tumor matrix patterns were mostly homogeneous and cloudlike. Four of the lesions were expansile. All cases had cortical thinning and discontinuity. Extraosseous involvement of the soft tissues was present in six cases, and active periosteal response was present in two. High-grade malignancies subsequently developed in two patients--one was a local recurrence and the other was a distant metastasis. A broad spectrum of radiologic features was observed in well-differentiated intramedullary osteosarcomas, but all cases had at least two of three radiographic findings (cortical discontinuity, poorly marginated soft-tissue extension, or cloudlike tumor matrix pattern) that strongly suggested malignancy.

Adolescent↗
PubMed1982

The effect of prolonged ischemia time on osteocyte and osteoblast survival in composite bone grafts revascularized by microvascular anastomoses.

Twenty-six adult mongrel dogs were used to investigate the effect of prolonged ischemia on osteocyte and osteoblast survival in composite bone grafts revascularized by microvascular anastomoses. The bone grafts were stored in chilled (+5 degrees C) Collins-Terasaki solution, and ischemia time varied from 90 minutes to 48 hours. With histologic techniques and fluorochrome bone labeling it was shown that the bone marrow, the osteocytes, and the osteoblasts could completely survive up to 25 hours of ischemia. Although bone grafting techniques are employed routinely in many orthopedic and plastic surgical procedures, our knowledge is incomplete concerning the ischemic period tolerated by osteocytes and osteoblasts in these grafts. This information is essential when bone transfer on microvascular pedicles is employed, since 3 to 6 hours of ischemia time may be necessary in these operations. The present experiment has shown that osteocytes and osteoblasts can survive a rather long ischemic period if the medullary nutrient blood supply is later reconstituted. This fact adds validity to the technique of free vascularized bone grafts in reconstructive surgery.

Animals↗
PubMed1982

Free vascularized bone grafts: factors affecting their survival and ability to heal to recipient bone defects.

Composite bone grafts revascularized by microvascular anastomoses with respect to bone cell survival, vessel architecture, and the grafts' ability to participate in healing toward a recipient bone were investigated in 21 adult mongrel dogs. Bone grafts with an intact medullary and periosteal supply were compared with bone grafts with only periosteal bone supply intact. It was demonstrated that bone grafts with both medullary and periosteal blood supplies survived completely but were partially resorbed with time. Periosteally supplied bone grafts demonstrated less resorption but showed, in some grafts, bone marrow necrosis and partial loss of osteocytes. The osteoblasts survived equally well in both groups. No difference in the ability to participate in healing to a recipient bone defect could be demonstrated, although microangiograms revealed significant differences in the medullary microvasculature and at the host-graft junctions in these two types of grafts. The question of whether to preserve the medullary blood supply when transferring whole bone segments by microvascular anastomoses is significant with respect to possible donor-site selection. This study has shown that bone grafts with an intact medullary and/or periosteal blood supply will survive transplantation and participate in healing to a recipient defect equally well.

Animals↗
PubMed1982

Microvascular free bone transfer with revascularization of the medullary and periosteal circulation or the periosteal circulation alone. A comparative experimental study.

UNLABELLED: Two different types of vascularized rib grafts presently are used in clinical practice and as experimental models for investigations on free microvascular bone transfer: the posterior rib graft, including both medullary and periosteal blood supply to the bone; and the posterolateral segmental rib graft, supplied by periosteal vessels alone, Complete survival of bone after successful revascularization of the posterior type of graft is well established, but this graft has the disadvantage of a complicated dorsal dissection which has limited its clinical use. Instead, many microsurgeons have utilized the posterolateral rib segment, which is easy and safe to excise although its viability and adequate microcirculation have not yet been confirmed. In nine large dogs, we compared the viability and vascularity of bone after transfer of the two types of bone grafts by histological methods, fluorochrome bone-labeling, microangiography, and technetium scintigraphy. The grafts were transferred to the subcutaneous fat tissue in the groin, where blood supply was reconstituted by microvascular anastomoses to local donor vessels. The results suggest that a bone transplant with revascularization of periosteal only established a collateral circulation to medullary vessels, and that there is no difference in viability of the two kinds of grafts. CLINICAL RELEVANCE: The technique of transferring whole bone segments by microvascular anastomoses of their vascular pedicles has been employed clinically either by preserving the periosteal blood supply alone or by preserving the medullary and the periosteal blood supply. This study demonstrates that the preservation of the periosteal blood supply alone can result in complete bone-graft survival even when the graft is placed in a poorly vascularized tissue bed.

Animals↗
PubMed1981

The effects of storage media and perfusion on osteoblast and osteocyte survival in free composite bone grafts.

Twenty-two adult mongrel dogs were used to investigate the effects of storage alone and storage and perfusion in 3 different storage media on the survival of osteocytes and osteoblasts in free bone grafts revascularized by microvascular anastomoses. Evaluation of the grafts at 2 weeks demonstrated that storage in chilled (+ 5 degrees C) physiologic saline or Collins-Terasaki solution resulted in greater survival of osteocytes and osteoblasts than did storage in chilled BGJb solution or in room temperature air. No beneficial effect could be detected from perfusing the bone grafts with their storage media. On the contrary, deleterious effects were noted at the sites of the anastomoses, with thromboses of vessels secondary to endothelial damage.

Animals↗