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Biomedical subjects

H E Busk

Publications and source records attributed to H E Busk.

10 recordsLinked to original sources
PubMed1986

Defective monocyte function in Legionnaires' disease complicating hairy cell leukaemia.

We describe a case of Legionnaires' disease in a 64-year-old man, in which hairy cell leukaemia was diagnosed after the onset of the infection. Immunological studies revealed a complete suppression of blood monocyte chemotactic and oxidative burst activities. We suggest that in hairy cell leukaemia both monocytopenia and defective functions of monocytes underlie the increased susceptibility to intracellular infections including Legionnaires' disease.

Diabetes Mellitus, Type 2↗
PubMed1984

Rapid identification of Capnocytophaga isolated from septicemic patients.

Four Capnocytophaga strains from blood cultures of immunocompromised patients with malignant disease and the type strains of three Capnocytophaga species were examined and compared to strains representing five other genera that are hard to differentiate from Capnocytophaga. With three rapid identification methods, negative catalase and oxidase reactions and positive ONPG assay, Capnocytophaga was easily separated from Eikenella corrodens, Actinobacillus actinomycetemcomitans, Cardiobacterium hominis, and CDC group DF-2. Haemophilus aphrophilus was excluded by leucine, valine and cystine arylamidase and alpha-glucosidase reactions (API ZYM). Further confirmatory reactions constituted gelatin hydrolysis, haemin requirement, and carbohydrate and esculin breakdown. Although rapid identification of Capnocytophaga to the genus level was feasible, differentiation on a species level proved impossible.

Capnocytophaga↗
PubMed1984

The cultivation and rapid enzyme identification of DF-2.

The investigation of two clinical isolates and two reference strains of DF-2 showed that supplementary cysteine and incubation in a humid atmosphere were important growth-promoting factors for these fastidious, gram-negative bacteria. Broth-base media with phenol red indicator were proven to be satisfactory for carbohydrate fermentation tests. Two four-hour enzyme assays (API ZYM and Rosco Diagnostic Tablets) were used to compare the enzymatic activity of DF-2 with that of 27 species of other non-enterobacterial organisms. The Rosco assay revealed that only the DF-2 strains had a positive alpha-fucosidase reaction, suggesting that this character may provide the means for rapid characterization and identification of these bacteria and also be of value for taxonomic classification. The incongruent results of the API ZYM assay seem to be due to the different substrates of the two assay systems.

Bacteriological Techniques↗
PubMed1975

The incidence of lactose malabsorption in ulcerative colitis.

120 Danish patients with ulcerative colitis, admitted consecutively to a Department of Gastroenterology, were investigated for lactose malabsorption. The prevalence was 9.2 percent, which is not significantly higher than that in a mixed Danish gastroenterological material, There was no difference in distribution according to age and sex, and the incidence was not correlated to the severity of the ulcerative colitis. The possible reasons for the wide variation in the incidence of lactose malabsorption in materials of patients with ulcerative colitis are discussed, and it is concluded that the main cause must be that the materials with the highest incidence include patients of races and ethnic groups in which lactose malabsorption is a common finding.

Adult↗