A short history of the British army veterinary services.
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Biomedical subjects
Publications and source records attributed to H E Carter.
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Zygotes and ookinetes of the rodent malaria Plasmodium berghei can be enriched 50-fold, from whole blood cultures by ammonium chloride lysis. Three monoclonal antibodies (MoAbs) raised against such enriched preparations specifically bind to a determinant of Mr 21 kD as assessed by 125I-labelled goat anti-mouse IgG probed immunoblots of Western transfers of SDS-PAGE gels. Indirect immunofluorescence indicates that the 21 kD determinant bound by specific MoAbs, whilst not detectable on gametocytes or gametes, appears on the parasite surface within 2 h of exflagellation/fertilization and increases thereafter. The three MoAbs specifically binding the 21 kD determinant block oocyst development in mosquitoes by at least 90%, as assessed either by in-vitro membrane feeds or by live feeds on passively immunized mice. These MoAbs reduce ookinete formation in vitro by between 52 and 100%. Possible mechanisms of action of these MoAbs are discussed.
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The inositol lipids of plant seeds consist of phosphatidyl inositol, the phytoglycolipids, and a previously uncharacterized ceramide-phosphate-polysaccharide. These three species have been separated from each other and from the common glycerophosphatides by a series of simple countercurrent distributions, first as the naturally occurring Ca-Mg salts and subsequently in the Na salt form. The new ceramide-phosphate-polysaccharide is present in each of the four plant phosphatides examined (corn, soybean, flax, safflower). It is devoid of glucosamine but contains the other carbohydrate components commonly found in the phytoglycolipids. The basic structural unit of the new glycolipid consists of a ceramide-phosphate-inositol-hexuronic acid moiety to which the other sugars (galactose, mannose, arabinose) are attached. Flax ceramide-phosphate-polysaccharide has fucose in addition to the other sugars.