PubMed Health⌕ Search

Biomedical subjects

H E Graves

Publications and source records attributed to H E Graves.

7 recordsLinked to original sources
PubMed1980

Interferon production by human-mouse hybrid cells: dominant mouse control of superinduction and priming.

We have examined the production of interferon by a number of human-mouse hybrid clones in response to polyriboinosinic acid:polyribocytidylic acid copolymer [poly(rI).poly(rC)] all of which produced both human and mouse interferons when stimulated with a virus. Their capacity to be superinduced and primed for interferon production in response to poly(rI).poly(rC) was compared to that of the parental human and mouse cells. It was found that the hybrids responded in a way similar to their mouse cell parents, indicating dominant mouse control of both the priming and superinduction phenomena.

Animals↗
PubMed1979

Somatic cell genetics of human interferon production in human-rodent cell hybrids.

Forty-two primary human-mouse cell hybrids, derived in two separate experiments, were treated with Newcastle disease virus (NDV): eight hybrids were found to produce human interferon and this was shown in every case to be predominantly of the fibroblast type. An extensive analysis was made in terms of karyotype and marker enzymes on all the eight hybrids producing interferon and also on five hybrids which did not produce interferon, five randomly selected hybrids and eleven subclones resistant to diphtheria toxin. The results suggest that, contrary to previous reports, a gene on chromosome 5 is not involved in production of human interferon. Its production was however correlated with the presence of chromosome 9 in the hybrids. Analyses of two sets of human-Chinese hamster hybrid subclones from two different crosses were also consistent with the assignment of a human interferon gene to chromosome 9.

Animals↗
PubMed1979

Interferon production: variation in yields from human cell lines.

A survey of human diploid, aneusomic, transformed and tumor fibroblast or fibroblastoid cell lines for their capacity to produce interferon after polyriboinosinic acid:polycytidylic acid induction showed considerable variation in responsiveness. There was no apparent correlation between karyotype or phenotype and interferon production. Pretreating or "priming" the cells with human interferon generally led to increased yields of interferon after polyriboinosinic acid:polycytidylic acid induction in all cell lines tested. All the cells also showed the "super-induction" phenomenon, although to varying degrees. The combination of priming and superinduction conditions led to the production of very high yields of interferon in some cell lines, but in other lines, yields less than either the primed or superinduced amounts were found. A more limited survey of human cell lines for their capacity to produce interferon after Newcastle disease virus induction also showed that yields varied from line to line. However, there was little evidence to suggest that ability to produce interferon after Newcastle disease virus induction correlated with that after polyriboinosinic acid:polycytidylic acid induction.

Cell Line↗
PubMed1976

Toleracne to equine IgG after immunosuppression in patients with multiple sclerosis.

Fourteen patients with multiple sclerosis were given two tolerizing doses of equinine gammaglobulin followed by equine antilymphocyte globulin. Twelve were sufficiently tolerant to have no clinical symptoms of hypersensitivity, no skin reactivity and normal plasma clearance rates. Some tolerance was lost in the following months revealed by immediate wheal and flare skin reactions. No correlation between success in tolerance induction and beneficial effects on the disease process could be established in the short term follow-up period of this pilot study.

Animals↗
PubMed1976

The detection of F(ab')2-related surface antigens on the thymocytes of children.

A Fowl antiserum to human polyclonal F(ab')2 with specificities for variable region antigens of both light and heavy chains and for the constant portion of the Fd fragment bound to about 20 per cent of thymocytes from three children. Binding was completely inhibited by purified human polyclonal IgG and only partly by L chains. The fowl anti-F(ab')2 also bound to a subpopulation of peripheral blood lymphocytes which was somewhat larger than the B-cell population identified with other anti-immunoglobulin sera. Binding was detected by autoradiography and the amount of radiolabel seen on thymocytes was nearly as great as that on peripheral blood lymphocytes. As no binding to thymocytes was seen with other anti-immunoglobulin sera we propose that the fowl anti-F(ab')2 recognizes immunoglobulin or material with structural similarities which is synthesized by thymocytes but of which predominantly V region determinants are exposed on the membrane surface.

Animals↗
PubMed1975

Intensive immunosuppression in patients with disseminated sclerosis. II. Tolerance to equine IgG and effect on immunoglobulin and complement levels.

Fourteen patients with multiple sclerosis were given two tolerizing doses of equine gamma-globulin before treatment with anti-lymphocyte globulin. Twelve patients had no clinical symptoms of hypersensitivity and most of them had no detectable antibody to the equine globulin until several months later. Even then, antibody could only be shown by immediate type skin reactions but not by any in vitro assay. We conclude that a state of partial tolerance was established in these patients for the period of treatment but that later they may develop traces of hypersensitivity.

Animals↗