Sensitivity of bacteria to 16 antibiotic agents.
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Biomedical subjects
Publications and source records attributed to H E Rhoades.
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Newborn, germfree piglets were susceptible to Escherichia coli associated with human, nonenteric infections and should provide a useful model in the study of generalized E. coli infections.
Antimicrobial agents were added to the feed of swine for three weeks to determine the interrelationships of potentially pathogenic agents in the nasal tract, turbinate atrophy and weight gains. Bordetella bronchiseptica was not isolated from the groups fed the combination of chlortetracycline, penicillin and sulfamethazine. B. bronchiseptica was found in some pigs after the feeding trail, but this organism was not significantly associated with turbinate atrophy at the time of slaughter.Mycoplasma hyorhinis was not found in the nasal passages of the pigs that received feed containing high concentration chlortetracycline but was found in pigs that received other diets. Hemophilus suis was not significantly reduced by any of the treatments used. The organisms studied in the pigs were not isolated from the personnel handling the pigs.
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In a group of 112 cats, examined during an outbreak of diarrhea, 104 (92.8%) yielded Escherichia coli with identifiable O groups as compared to 12 (21.8%) of 55 control cats apparently free of intestinal ailments. E. coli group O6 occurred in 49.1% of the cats with diarrhea, and persisted in this group of cats at about the same rate when examined for a period of ten months. E. coli group O6 was not isolated from the control group. Specimens were also collected from dogs with various clinical signs. E. coli O4 and O6 strains appear to be important potential pathogens for canine species and were found to spread from one dog to another when in close contact. E. coli group O4 was identified in ten and O6 in three of 14 dogs examined. All isolates in which the O group could be identified were hemolytic.
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Diarrhea, reduced appetite, and nervousness were observed following oral exposure of six pigs to freeze-thaw extract and living culture of E. coli strain 0139:K82:H1. The most significance gross change was the appearance of subserosal edema of the mesentry, especially of the mesocolon. Histological findings included perivascular edema of the brain and reticulum cells as well as lymphocytic aggregates in the renal cortex simulating early neoplasia. Edema of the submucosa of gallbladder, the duodenum, the spiral colon and the fundic stomach as well as the lymph nodes was also observed. The syndrome which was produced resembled, in many aspects, edema disease although some complicating factors were ob-
Twenty-two pigs were inoculated parenterally with various E. coli 0139:K82:H1 preparations. Clinical signs of disease in pigs injected with freeze-thaw extract consisted of early listlessness, diarrhea and, later, hyperirritability of varying intensity in some animals.Hemorrhagic gastroenteritis involving the duodenum, spiral colon and the fundic portion of the stomach, and ulceration of the fundic stomach were observed at post-mortem examination of pigs inoculated parenterallly with living culture or freeze-thaw extract. No significant lesions were observed in pigs inoculated with ultrasonic or hypotonic acid-saline extract. In pigs injected with living culture or freeze-thaw extract, the histological alterations consisted of moderate perivascular edema of the brain, marked hepatic parenchymal cell degeneration, hepatic subserosal edema and "toxic" lymph nodes, when compared to the control group.
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