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H E Stegner

Publications and source records attributed to H E Stegner.

At least 55 records · Page 3Linked to original sources
PubMed1983

9. Steroid receptors. Correlation of estrogen and progesterone receptor status with ultrastructural differentiation in breast tumors.

The interrelationships of ultrastructural and biochemical parameters have been studied in a multivariate analysis of 139 cases of invasive breast carcinoma. There was no relationship between the histologic subclassification and distribution of estrogen and progesterone receptors. However, significant correlations could be found between receptor status and certain ultrastructural features of the tumor cells. A prevalence of well differentiated cancers had a significant correlation with a positive estrogen receptor status. Estrogen receptors were more frequently associated to tumors showing great complexity of cytoplasmic organization, high degrees of cell interconnections by desmosomes and membrane interdigitations, presence of intracytoplasmic ductules, specific secretory granules, and low grades of nuclear pleomorphy. In a series of 120 non-selected cases, receptor status was assessed by the fluorescent technique. Of the cases, 38.3% have proved to be ER+, 49.2% showed low binding capacity and 12.5% were negative. The correlation of receptor content with the histological type of breast cancer revealed some type-dependent connections. While only 36.9% of the solid tumors were receptor positive the proportion increased to 54.2% for the more differentiated glandular type of ductal carcinoma. 8 out of 13 (61.5%) infiltrating lobular carcinomas and 4 (4) colloid cancers were found to be receptor positive. The plotting test showed a good correlation of histochemical receptor status and histological grading. Although the type of "receptor", which is localized by the indirect fluorescent technique is still incompletely defined, there is no doubt, that the selective binding affinity reflects a specific capacity of the tumor cells depending on their degree of differentiation.

Breast Neoplasms↗
PubMed1981

Paget's disease of the vulva with underlying apocrine adenocarcinoma and local lymph node invasion.

A case of Paget's disease of the vulva with an underlying apocrine adenocarcinoma and local lymph node invasion is reported in a female aged 70. Subjective symptoms of pruritus, burning and pain were associated with typical, but unspecific clinical findings of an erythematous, indistinctly limited, weeping lesion of palm-size with small superficial erosions. Near the right labium majus a solid measuring 2.5 X 1.5 cm in diameter was palpable. The diagnosis was established by microscopic examination of biopsy material, showing intraepidermally the typical Paget's disease with a subjacent, highly differentiated apocrine adenocarcinoma of the sweat glands combined with inguinal lymph node invasion. Other epidermal malignancies were excluded by histochemical reactions. For therapy, telecobalt irradiation combined with high energy electron was applied.

Adenocarcinoma↗
PubMed1981

Prescreening of cervical smears using two-parameter flow cytometry. Cytologic identification of artifacts.

Individual properties of gynecologic specimens can produce artifacts in flow cytometric (FMC) measurements, possibly leading to false interpretations. An identification of such artifacts was undertaken by parallel FCM and microscopic investigations. One hundred fifty unselected cervical smears were measured by FCM using the fluorochromes 4'-6-diamidino-2-phenylindole (DAPI) for DNA and sulforhodamine (SR 101) for protein. Microscopic specimens were stained by the Papanicolaou technique, and a detailed cytogram was prepared from each smear. FCM discrimination of fluorochrome-stained superficial and intermediate cells was very difficult. On the other hand, a correlation could be established between the fraction of cells from the deeper epithelial layers in the microscopic cytogram and the mean protein content in the FCM histogram. Furthermore, the role of microorganism could be elucidated. Some microorganisms may produce a reduction of the protein content by cytolytic changes. Other microorganisms adhere to the cell surface, resulting in a misleading increase of the DNA fluorescence. Implications for the problem of false alarms are discussed.

Bacteria↗
PubMed1980

[Clinical aspects of uterine sarcomas (author's transl)].

Uterine sarcomas constitute 1% to 3% of all uterine cancers. There are three main histological varieties: endometrial stromal sarcoma, leiomyosarcoma, and mixed mesodermal (Müllerian) sarcoma. The tumors are characterized by local aggressiveness and early dissemination. According to the literature, 5 year survival rates range from 3 to 75% (!). This wide range reflects great prognostic differences in the various histologic types. On the other hand it may be due to discrepancies in histopathological evaluation. Radical surgery is the most effective therapy. In early stages it leads to 5 year survival rates of up to 88%. Adjuvant irradiation may increase the control of disease in the pelvis but has proven to be of little influence on the final outcome. The histologic variants show great differences in radiosensitivity. In endometrial stromal sarcomas adjuvant radiation therapy apparently can improve the treatment results. By contrast mixed mesodermal sarcomas are highly radioresistent. There is poor knowledge on the effectiveness of cytotoxic agents on uterine sarcomas. Objective remission of distant metastasis has been observed following treatment with cytotoxic antibiotics (Adriamycin).

Adult↗
PubMed1979

Flow cytometric prescreening of cervical smears.

One-parameter (nuclear DNA) and two-parameter (nuclear DNA and protein or cellular light scatter) measurements of cervical smears were performed using an ICP 11 and a cytofluorograf 4800 respectively. A total of about 1000 cases was analyzed. For the estimation of nuclear DNA alone two fluorochromes were tested (ethidium bromide (EB) and mithramycin (MMC)) combined with three different methods of cell preparation. For the two-parameter measurements cells were double stained with EB and fluorescein isothiocyanate (FITC). Red fluorescence (EB) versus green fluorescence (FITC) or red fluorescence versus scatter were recorded. A computer analysis of the one-parameter histograms was performed using discriminant analysis and the results were compared with the cytodiagnosis of microscopic specimens stained with the Papanicolaou technique. The error rates of the flow cytometric (FCM) data were as follows: (a) standard EB staining, 11% false negative, 26% false positive, 6% unsatisfactory results; (b) pepsination of vital cells and EB staining, 12% false negative, 14% false positive and 4% unsatisfactory results; (c) MMC staining, 10% false negative, 65% false positive and 5% unsatisfactory results. Our two-parameter measurements prove that, as confirmed by cell sorting, red fluorescence versus scatter allows separation of at least three subpopulations in most analyzed samples: (a) anucleated cells; (b) leukocytes; and (c) intermediate and superficial cells.

Cell Nucleus↗
PubMed1977

[Decidualisation of the endometrial stromal cell. An electron microscopic study (author's transl)].

The fine structural changes of the endometrial stromal cell from late secretory phase up to full developed decidua cell have been investigated by means of electron microscope. During decidual transformation the cell volume, the assortment of cytoplasmic organelles and the mode of intercellular attachments undergo characteristic alterations. These alterations reflect an adaptation on new cellular functions. In contrast to the structural organization, which is transformed continuosly, the encymatic cell pattern exerts a step wise gradual transformation, since new encymatic capacities can only arise after their funtional structures have been established. To the most important functions of decidua cells and their prestages belong secretory and endocrine activities. Precollagen fibrills represent a specific secretory product of the stroma reticulum cells. The active involvement of the full differentiated decidua cells in steroid metabolism is indicated by characteristic transformation of the cytoplasmic organization concomittantly to the appearance of intracellular steroid dehydrogenase activities. So-called karyosoma could be demonstrated within the nuclei of postovulatory stromal reticulum cells as in decidua cells. Similar nuclear differentiations have been observed in various target cells of steroid hormones. They possibly are structural manifestations of the hormonal interaction with the nuclear DNA.

Adolescent↗