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H Eiler

Publications and source records attributed to H Eiler.

45 records · Page 3Linked to original sources
PubMed1977

Sex steroids in follicular fluid and blood plasma during the estrous cycle of pigs.

Concentrations of estrogen (E), progestin (P),and androgen (A) were determined in follicular fluid (FF), and concentrations of E and P were measured in peripheral plasma and perifollicular plasma during the estrous cycle in the pig. Also, concentrations of E, P,and A were determined in the utero-ovarian vein (U-OV) plasma every 2h during estrus period in pigs. It was observed that as follicles increased in size, the concentration of E, P, andA in the FF rises from 13,32 and 1 ng/ml at day 8 of the cycle to 180, 754, and 21 ng/ml at day 20 of the cycle, respectively. Approximately 12 h before ovulation there is a significant (P less than 0.01) drop of E, P, and A in the FF to 27, 120, and 0.77 ng/ml, respectivley. Concentrations of E and P in FF and peripheral plasma do not correlate during the second half of the estrous cycle. At day 20 of the cycle, E and P concentrations in the plasma were 20 pg/ml and 0.4 ng/ml, respectively. Concentration of P in the U-OV plasma started increasing 18 to 24 h after the onset of estrus, which is coincident with the drop of P concentration in the FF. As estrus progressed there was a linear drop of E concentration (r = 0.90) in the U-OV plasma. E and P concentrations in the FF were found to be about 1000 times greater than those in the peripheral blood and 5 times greater than in the blood obtained from the perifollicular capillary network at the end of the cycle.

Androgens↗
PubMed1977

Oestrogen content of semen and the effect of exogenous oestradiol-17beta on the oestrogen and androgen concentration in semen and blood plasma of bulls.

The concentration (mean +/- S.E.M.) of oestrogen in semen (0-89 +/- 0-04 ng/ml) of bulls was 8-9-fold higher than in blood plasma (0-10 +/- 0-01 ng/ml) while the concentration of androgen in semen (1-15 +/- 0-26 ng/ml) was 2-7-fold lower than in blood plasma (3-21 +/- 0-47 ng/ml). This concentration of oestrogen in semen was not influenced by 0,1,3 or 5 false mounts or when ejaculations occurred at 2-min intervals. When 100 mg oestradiol-17beta were injected i.v. the oestrogen level in semen followed that in the plasma.

Androgens↗
PubMedDate not supplied

The potential of collagenase as a new therapy for separation of human retained placenta: hydrolytic potency on human, equine and bovine placentae.

The purpose of this study was to determine to what degree bacterial collagenase may digest human placentae compared to equine and bovine placentae. Placenta samples from human, equine and bovine were incubated with bacterial collagenase solution at various concentrations. The degree of hydrolysis and collagen breakdown was measured by the release of total proteins and hydroxyproline into the incubation media. Also, whole placentae were injected via umbilical cord arteries with collagenase solution (200 U/ml, 200 ml total volume in human and 1000 ml in equine) and hydrolysis determined chemically and subjectively. Human and equine placental collagens were the most sensitive to collagenase digestion. Overall mean collagenase activity determined by the release of hydroxyproline from human placenta was 1.6 times and in equine placenta three times greater than in bovine placenta, while the breakdown of non-collagenous proteins remained negligible. When injected into whole placenta, the collagenase digested placentae evenly within 6-12 h. At 24 h, placentae were liquefied, although, umbilical blood vessels resisted collagenase digestion. Bacterial collagenase was highly effective in breaking down human placenta collagen. Intraplacental injections of collagenase via umbilical cord arteries may help to detach retained placenta in women as it does in mares and cows.

Animals↗
PubMedDate not supplied

Evaluation of inhibitory effects of doxorubicin on collagenase using a bovine placentome model.

The chemotherapeutic antibiotic doxorubicin (Adriamycin) was reported to inhibit bacterial collagenase activity when tested on both a synthetic substrate and intact collagen. Our objective was to establish whether doxorubicin would inhibit bacterial collagenase activity on a bovine placentome model. Metabolically active, isolated bovine placentomes were infused, via umbilical vessels, with a mixture of bacterial collagenase and doxorubicin. Six experimental groups were used. Group (1) comprised of saline controls; (2), collagenase at 1,200 U/cc; (3, 4, and 5), collagenase (1,200 U/cc) plus doxorubicin at 0.04 mg/cc, 0.02 mg/cc, 0.01 mg/cc, respectively; (6), doxorubicin at 0.02 mg/cc. After 5 hrs of incubation (39 degrees C), manometric pressure (needed to separate caruncle and cotyledon), caruncle-cotyledon interface hydroxyproline (collagenolysis), and total protein (proteolysis) were determined. Results indicated no (P > 0.01) inhibition of collagenase by doxorubicin. We concluded that doxorubicin (at above dosages) is not an inhibitor for bacterial collagenase when tested on bovine placentomes.

Animals↗