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H Emmerich

Publications and source records attributed to H Emmerich.

17 recordsLinked to original sources

Dewetting hydrodynamics in 1+1 dimensions.

A model for the phase transition between partial wetting and dewetting of a substrate has been formulated that explicitly incorporates the hydrodynamic flow during the dewetting process in 1+1 dimensions. The model simulates a fluid layer of finite thickness on a substrate in coexistence with a dry part of the substrate and a gas phase above the substrate. Under nonequilibrium "dewetting" conditions, the front between the dry part and the wet part of the surface moves towards the wet part inducing hydrodynamic flow inside the wet layer. In more general terms, the model handles two immiscible fluids with a freely movable interface in an inhomogeneous external force field. Handling the interface by a new variant of the phase-field model, we obtain an efficient code with well-defined interfacial properties. In particular, the (free) energy can be chosen at will. We demonstrate that our model works well in the viscosity range of creeping flow and we give qualitative results for the higher Reynolds numbers. Connections to experimental realizations are discussed.

Journal Article↗

The uptake and release of ponasterone A by the Kc cell line of Drosophila melanogaster.

The association of ponasterone A (PNA) and 20-hydroxyecdysone with Kc cells is commensurate with their biological activity on this Kc cell line, the physiological activity ratio for PNA, 20-hydroxyecdysone and ecdysone is 1 : 50 : 2000, resp. Both association and release of [3H]-PNA are temperature-dependent, the activation energy was calculated as 16.7 cal (Arrhenius analysis). This association is compatible with unlabelled PNA and various ecdysteroids. The KD for PNA (Scatchard analysis) was estimated as 3.6 x 10(-9) M, giving the number of binding sites as approx. 1800 per cell.

Cell Division↗

Immunohistochemical localisation of ecdysteroids in the follicular epithelium of locust oocytes.

Frozen sections of growing terminal follicles of the locust ovary were incubated with an ecdysteroid-specific rabbit antibody and the bound antibody visualised by the use of FITC-labelled goat-anti-rabbit antiserum. A bright fluorescence was seen in the cytoplasm of the follicle cells in terminal follicles with a length between 4.0 and 6.0 mm with a maximum intensity at 5.5 mm, indicating the presence of ecdysteroids in these cells in this particular developmental stage.

Animals↗

A radioimmunoassay for arthropod moulting hormones, introducing a novel method of immunogen coupling.

Inokosteron-26-oic acid was coupled to thyroglobulin in aqueous pyridine by a water-soluble carbodiimide. After exhaustive dialysis and gel filtration on Sephadex G-25 in the presence of sodium dodecylsulfate, a coupling ratio of 164 haptens per molecule of thyroglobulin was determined. In all three animals injected with the conjugate, ecdysone-binding antibodies were detected. After one booster injection the antiserum could be diluted 1 : 5000 (1 : 4000, or 1 : 2000) in order to get a 50% binding of [3H]ecdysone. The dissociation constant was calculated as 5.8 X 10(-10) MOL/L. The antiserum has a greater affinity for ecdysone and 22-isoecdysone than for all other ecdysteroids and steroids tested.

Animals↗

Derivatives of the insect moulting hormone for affinity chromatography, and their biological activities.

Several derivatives of the arthropod moulting hormone have been synthesized which were coupled to AH Sepharose 4B yielding about 2 mumole ligand per g wet gel. As an indication of the suitability of the ligands for biological work the puff inducing capacity of their methyl esters was tested. The methyl ester of inokosterone-C-26-carboxylic acid possesses the highest biological activity; lower activities were obtained with the esters of ecdysterone-C-6-CM-oxime and ecdysterone hemisuccinates. Therefore, inokosterone-C-26-carbocylic acid should be a useful ligand for affinity chromatography of ecdysone recptors from insect tissues.

Cholestenes↗