PubMed Health⌕ Search

Biomedical subjects

H Endoh

Publications and source records attributed to H Endoh.

At least 109 records · Page 6Linked to original sources
PubMed1986

Naloxone attenuates hypoxic depression of ganglionic transmission.

The effect of the opioid antagonist naloxone on hypoxia-induced blockade of synaptic transmission in the superior cervical ganglion (SCG) of the rat was studied in vitro. Naloxone (6 X 10(-6) M or more) attenuated the hypoxia-induced blockade of synaptic transmission in the SCG. In addition, in the concentrations studied, naloxone itself had a blocking effect on ganglionic transmission that involved terminal sites of the preganglionic axons. These data suggest that the protective effect of higher doses of naloxone on the hemodynamic responses to hypoxemia or ischemia may originate at least in part from the attenuating effect of naloxone on the hypoxia-induced blockade of ganglionic transmission.

Action Potentials↗
PubMed1985

[Transfer of ceftizoxime to renal tissues].

Serum and renal tissue concentrations of ceftizoxime (CZX) after intravenous dosing with 1 g were determined in 15 patients with renal disease. The highest serum concentration of CZX was 105 micrograms/ml 6 minutes after dosing and the lowest concentration was 10.5 micrograms/ml at 170 minutes. The highest renal tissue concentration was 155 micrograms/g and the lowest concentration was 7.46 micrograms/g. There were no correlation between the tissue concentrations and sampling time. Tissue to serum concentration ratios ranged from 0.28 to 5.73. These patients were divided into 3 groups; hydronephrosis, normal kidneys and renal atrophy according to pyelographic findings. Their renal tissue concentrations were compared with serum concentrations in each group. The tissue concentrations were lower than the serum concentrations in the first group, and were almost the same or higher in the other groups. CZX concentrations in the medulla and the cortex were determined in 5 patients; and were higher in the medulla in 4 of these patients. Urinary tract infection due to E. coli, K. pneumoniae, P. mirabilis, S. pyogenes or S. epidermidis was present in 5 of the 25 patients. Intravenous injection of 1 g of CZX provided therapeutically effective concentrations against these urinary tract infections.

Adult↗
PubMed1984

Continuous blood-letting for congestion in replantation of the amputated finger.

Experimental and clinical studies have been made on the function of the venous system in the replantation of the contused severed finger. In experiments on rats replantation of severed limbs was more successful in a group treated by continuous blood-letting and anastomosis of the artery alone, compared with controls in which only the artery was anastomosed. Histologically, in the first group there was less congestion, thrombus-formation, oedema and exudative haemorrhage in the initial stage; in the reparative phase there was less granulation and the degree of muscular degeneration was very slight. Clinically, contused, severed fingers were successfully replanted when venous anastomosis was impossible or incomplete, by using the fish-mouth incision for continuous blood-letting. The resulting scar caused no problem aesthetically or functionally.

Adult↗
PubMed1983

Coating of liposomes with subunits of monoclonal IgM antibody and targeting of the liposomes.

SH-bearing subunits of IgM antibody were coupled through the Fc portion to sonicated liposomes consisting of N-(m-maleimidobenzoyl)dipalmitoylphosphatidylethanolamine, dipalmitoylphosphatidylcholine and cholesterol. Liposomes coated with subunits of monoclonal IgM antibody against a mouse mammary tumor-associated antigen selectively bound to the antigenic target cells. Assays of the liposomes for complement dependent cytotoxicity and spleen cell binding revealed inactivation of the liposome-coupled antibodies in both assays.

Animals↗
PubMed1981

Antibody coating of liposomes with 1-ethyl-3-(3-dimethyl-aminopropyl)carbodiimide and the effect on target specificity.

Liposome surfaces were modified with normal rabbit IgG or rabbit anti-sheep erythrocyte IgG by adsorption or by coupling with a water soluble cross-linking reagent, 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDCI). Modification with normal IgG reduced the non-specific binding of the liposomes to erythrocytes. The immunological binding capacity of the immune IgG on liposomes was better for the chemically coupled preparation than for the adsorbed one. Complement dependent cytolytic activity of the immune IgG towards target erythrocytes was diminished as a consequence of liposome binding by either method, but to a lesser extent in the coupling method. Antibody coating by EDCI thus provides liposomes which can bind effectively to antigenic target cells.

Animals↗
PubMed1980

Agglutination microassay of hapten- or protein-modified liposomes using a multiple cell culture harvester.

A simple agglutination microassay is described for studying the presence of antigenic determinants on the surface of liposomal membranes. Antigen-sensitized radioactive liposomes, reacted with specific immune IgG in the wells of a microtest plate, were passed through a glass fiber filter in a multiple cell culture harvester. The amount of agglutinate trapped on the filter as determined by the radioactivity, was proportional to the amount of antigen in the liposomes and to the amount of specific immune IgG in the reaction mixture. The specificity of the agglutination was determined by inhibition with hapten and carrier antigens. Liposomes actively sensitized with hapten-conjugated lipid, and passively sensitized with protein, were used in the assay. A new method of preparing protein-bound liposomes is also described.

Agglutination Tests↗
PubMed1976

An autopsy case of the Lesch-Nyhan syndrome: normal HGPRT activity in liver and xanthine calculi in various tissues.

An autopsied case of the Lesch-Nyhan syndrome did not indicate the specific pathological features except delayed physical development. 2. Xanthine calculi caused by allopurinol administration scattered in the kidneys, brain, thymus, and thyroid glands, but its excretion into urine was not observed during his life. 3. Activities of hypoxanthine-guanine phosphoribosyltransferase (HGPRT) in various tissues indicate complete deficiency, but HGPRT in liver was normal.

Adolescent↗
PubMedDate not supplied

Three-dimensional structure of the rat pancreatic duct in normal and inflammated pancreas.

We observed the corrosion casts of the Wistar rats' pancreatic ducts with scanning electron microscopy (SEM), and their conventionally fixed pancreatic tissue with SEM and transmission electron microscopy (TEM). These findings revealed the following facts about the three-dimensional structure of pancreatic duct. (1) The interlobular and intralobular ducts branch like a tree, and the intercalated ducts wind and fork into two branches, although parts of the intercalated ducts anastomose with each other. The intercellular secretory canaliculi extend from the central lumina, which run straight through the center of the acini, finally approaching close to the basement membranes of acini. (2) The lumina of pancreatic ducts (i.e., the interlobular up to the intercalated ducts) are cylindric and have smooth surfaces. The luminal surface of each epithelial cell, however, is decorated by numerous microvilli and a single cilium. The length of the latter tends to be short in proportion to the diameter of pancreatic duct. Moreover the epithelial cell surfaces, which border each central lumen, have various densities of microvilli. (3) The intraductal cilium core is provided with nine microtubules, which is different from the number of microtubules encountered within the cilium core of uterine tube or bronchial epithelium. The number of microtubules in the cross-sectioned intraductal cilia decreases toward the distal portion of cilia. SEM and TEM observations on WBN/Kob rats' pancreatic ducts suggest that increased pancreatic ductal pressure causes the helical shape of the pancreatic ductal lumen. Such a helical form might also be caused by the protrusion of epithelial cell boundaries into their lumen and the hypertrophy and hyperplasia of epithelial cells, thus leading to the formation of numerous depressions equipped with elongated cilia.

Animals↗
PubMedDate not supplied

Biphasic flow velocity pattern in the descending aorta in double aortic arch.

We report a case of double aortic arch in which the biphasic flow velocity pattern in the descending aorta changed to a monophasic flow velocity pattern after corrective surgery. The hypoplasia of left aortic arch might prolong the acceleration time of the flow velocity in the left aortic arch.

Aorta, Thoracic↗
PubMedDate not supplied

Production of cyclic nucleotides from normal human brain.

Concentrations of cyclic AMP and cyclic GMP in arterial and internal jugular venous (IJV) blood were determined at the resting wakeful state in thirty surgical patients without neurological deficits. The levels of cyclic AMP in artery and IJV were 32.1 +/- 3.0 and 40.0 +/- 4.1 pmol/ml (means +/- standard errors), respectively, while those of cyclic GMP in artery and IJV were 12.2 +/- 2.7 and 14.4 +/- 3.0 pmol/ml, respectively. Concentrations of both cyclic nucleotides in IJV were significantly higher (P less than 0.001) than those in artery. IJV-arterial differences for cAMP and cGMP were 7.9 +/- 1.7 and 2.1 +/- 0.5 pmol/ml, respectively. The results indicate that both cyclic nucleotides are constantly produced and released from the normal human brain.

Adolescent↗
PubMedDate not supplied

Relation between spinal cord and epidural blood flow.

To test applicability of monitoring regional spinal cord blood flow by measuring regional epidural blood flow, both were simultaneously measured by the hydrogen clearance method in response to changes in PaCO2 and mean arterial blood pressure in rats anesthetized with pentobarbital. An excellent correlation was found between regional spinal and epidural blood flow over a physiological range of PaCO2 (27.8-66.7 torr) and blood pressure (30-130 torr), while a poor correlation was demonstrated between regional spinal or epidural blood flow and regional muscle blood flow in response to these same physiological parameters. Moreover, the rates of change in regional spinal and epidural blood flows were almost the same in response to both PaCO2 and blood pressure. These results suggest that both regional spinal and epidural blood flow are regulated by a similar mechanism and suggest that regional spinal cord blood flow can be monitored by regional epidural blood flow.

Animals↗
PubMedDate not supplied

Detection of minute pleural fluid in the pleural retracted space associated with peripheral lung cancer: evaluation with MR imaging.

PURPOSE: To evaluate the efficacy of magnetic resonance imaging (MRI) in detecting minute pleural fluid in the pleural retracted space (PRS) associated with peripheral lung cancer. MATERIALS AND METHODS: Our subjects were 20 patients with peripheral lung cancer in whom thin-section CT in the lung window setting demonstrated lesions adjacent to the pleural surface, and who were referred for MR imaging. The imaging findings were retrospectively evaluated and correlated with the histopathologic specimens. Pleural fluid was aspirated for cytology under ultrasound (US) guidance in six patients. RESULTS: STIR MR images revealed water SI areas beneath the chest wall associated with the lung cancer, whereas, on CT images, lung cancer and minute pleural fluid in the PRS showed similar soft-tissue density without enabling easy differentiation. Two of the six patients who underwent aspiration cytology showed malignancy. All histopathologic specimens obtained from 18 patients who underwent surgery showed pleural retraction corresponding to the water SI areas on STIR images. Histopathological study revealed that the fibrotic focus of the tumor tended to occur more intensively when the shape of pleural retraction was thinner and deeper. CONCLUSION: Water SI areas on STIR images were thought to suggest pleural fluid retention in the PRS. MRI was sensitive in detecting minute pleural fluid in the PRS and may help to avoid overdiagnosis of chest wall invasion induced from peripheral lung cancers.

Adult↗
PubMedDate not supplied

Diagnosis of chest wall invasion by lung cancer: useful criteria for exclusion of the possibility of chest wall invasion with MR imaging.

PURPOSE: To compare the accuracy of thin-section CT, conventional static MR imaging (conventional MRI), and breathing dynamic echo planar magnetic resonance imaging (BDEPI) in evaluating lung cancer invasion to the chest wall. MATERIALS AND METHODS: Thin-section CT, conventional MRI, and BDEPI were performed preoperatively in 20 patients suspected of having primary lung cancers adjacent to the chest wall on conventional CT. The results of imaging findings were compared with those of surgical and histopathological findings. RESULTS: All patients were confirmed to have no chest wall invasion after surgery. By thin-section CT, 10 of 20 patients were correctly diagnosed as having no chest wall invasion (50% specificity). Two of the 20 patients were incorrectly diagnosed as having chest wall invasion by conventional MRI and BDEPI (90% specificity). CONCLUSION: When chest wall invasion is suspected on CT scans, static and breathing dynamic MRI are recommended to avoid false positive interpretations.

Adenocarcinoma↗
PubMedDate not supplied

Enhancement of the antitumor effect by the concurrent use of a monoclonal antibody and the protein-bound polysaccharide PSK in mice bearing a human cancer cell line.

The antitumor effects of a monoclonal antibody against a human cancer cell line and a protein-bound polysaccharide, PSK, obtained from cultured mycelia of Coriolus versicolor in basidiomycetes were examined. The IgG2a monoclonal antibody against the human colon cancer cell line colo 205 induced in vitro antibody-dependent macrophage-mediated cytotoxicity against the cancer cells, but only slightly suppressed the in vivo growth of the cancer cells. Concurrent use of PSK with the antibody enhanced the in vitro antibody-dependent macrophage-mediated cytotoxicity as well as the in vivo antitumor activity. These findings suggest that the combined use of a monoclonal antibody and PSK, which have different modes of action, may be useful in the treatment of cancer.

Animals↗