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Biomedical subjects

H F Hasenclever

Publications and source records attributed to H F Hasenclever.

At least 19 recordsLinked to original sources
PubMed1976

Colonization of soil by Histoplasma capsulatum: II. Effect of bat guano added to an area of reduced colonization.

Application of bat guano to an area with reduced but detectable colonization by Histoplasma capsulatum had no quantitative effect on the fungus. After seeding the guano treated area and an untreated area with a soil culture of the fungus, a quantitative effect was noted in the treated area but the period of survival did not appear to be extended. Twenty-four months after seeding, the fungus was no longer detectable in the study areas and they have remained negative for more than a year.

Animals↗
PubMed1975

Candid albicans associated with the gastrointestinal tract of the common pigeon (Columbia livia).

Candida albicans was cultured from the upper gastrointestinal tract of 44% and from the lower alimentary tract in 6% of 50 common pigeons (Columba livia). Thirty per cent of samples of pigeon droppings collected from different locations were positive for the yeast. The colony forming units for positive samples ranged from 2 times 10-2-2-8times 10-6 per g dry feces. The percentage of antigenic Groups A and B for the strains of C. albicans isolated was approximately the same as for strains isolated from human sources.

Animals↗
PubMed1974

Cellular immune activity of a galactomannan-protein complex from mycelia of Histoplasma capsulatum.

A galactomannan-protein complex was extracted from defatted mycelium of Histoplasma capsulatum with 0.25 N alkali at 25 C. It accounted for 7.5% of the cell dry weight in nondialyzable form and had a galactose-mannose ratio of 2:5. The complex containing 68% protein was separated into polysaccharide and glycoprotein components by ion-exchange chromatography. The galactomannan formed a single precipitate in immunodiffusion, sharing a common antigen with the glycoprotein which contained two precipitating antigens. The glycoprotein elicited delayed-type hypersensitivity in guinea pigs as measured by skin-test and macrophage migration inhibitory factor assay, with 5 mug resulting in 86% specific inhibition. The galactomannan was skin-test negative, but 10 mug resulted in 80% specific inhibition of migration.

Amino Acids↗
PubMed1974

Serological and cellular immune activity of peptidoglucomannan fractions of Candida albicans cell walls.

A two-stage extraction of isolated cell walls of C. albicans resulted in 45% solubilization into antigens of high molecular weight leaving a wall residue which also had antigenic properties. Ice-cold dilute alkali removed 25% of the defatted cell walls. The extract was nondialyzable, had a glucose-to-mannose ratio of 2:3 and an amino acid content of 7.32%, and was designated peptidoglucomannan (PGM). An additional 26% of the walls resistant to stage I were solubilized by sonic treatment yielding a fraction having a glucose-to-mannose ratio of 6:1, termed soluble mannoglucan (sMG). The residue after extraction and sonic treatment contained 10.9% mannose, which was the insoluble mannoglucan. The gel permeation behavior of PGM and sMG on BioGel A5M was similar; each contained two components, one estimated to exceed 5 x 10(6) molecular weight and a second smaller species. The soluble cell wall fractions were active in immunodiffusion and carried antigenic group specificity. Immunoelectrophoresis of PGM, sMG, and mannan revealed some heterogeneity. The insoluble mannoglucan had agglutinating activity. A distinctive immunodiffusion pattern of cell wall antigens was formed with the serum of a leukemic patient with candidiasis. All three cell wall antigens and mannan elicited delayed-type hypersensitivity as measured by skin-test and specific inhibition of macrophage migration. A dose of 25 mug of PGM was sufficient to inhibit 89.9% migration in the peritoneal exudates of guinea pigs immunized with cell walls, and 10 mug of PGM inhibited 91.7% migration in guinea pigs immunized with insoluble mannoglucan.

Amino Acids↗
PubMed1973

Serotypes in Saccharomyces telluris: their relation to source of isolation.

Three serotypes have been characterized with three reference strains of Saccharomyces telluris and designated as A, B, and C. One reference strain of Torpulopsis bovina, the imperfect form of S. telluris, belonged to serotype B. Strains (141) of S. telluris isolated from four columbid species were serotyped. All 98 strains of this yeast isolated from Columba livia (feral pigeon) belonged to serotype B. Three other columbid species, C. leucocephala (white-crowned pigeon), C. fasciata (band-tailed pigeon), and Zenaidura macroura (mourning dove) harbored strains of serotype C only. Serotype A was not isolated from any of the avian species.

Agglutination Tests↗
PubMed1972

Effect of an interferon stimulator, polyinosinic: polycytidylic acid, on experimental fungus infections.

IN THE PRESENT STUDY, THE ADMINISTRATION OF AN INTERFERON STIMULATOR, POLYINOSINIC: polycytidylic acid (In:Cn), increased the severity of both experimental Candida albicans and Coccidioides immitis infections in mice, as evidenced by earlier time of death and higher final mortality. In addition, kidney tissue levels of C. albicans at 3 and 5 days after infection were found to be about 40- to 300-fold higher in In:Cn treated mice than in control mice. Experimental Cryptococcus neoformans infection, however, was not significantly affected by In:Cn treatment.

Animals↗
PubMed1970

Precipitin Inhibition of Candida stellatoidea Antiserum with Oligosaccharides Obtained from the Parent Mannan.

Inhibition of immune precipitation with oligosaccharides obtained from Candida stellatoidea mannan has been used to provide more information about the haptenic groups of this serologically active polysaccharide. The oligosaccharides di-, tri-, tetra-, and a mixture of penta- and hexasaccharides were studied. The inhibitory activity of these materials was tested with two immune sera. With one serum, 0.8 mumole of the mixture of penta- and hexasaccharides inhibited the reaction by 87%, and, with the other serum, 0.4 mumole of the mixture inhibited the reaction by 99%. Monosaccharides were also tested with each antiserum and found to be noninhibitory. It is apparent that the mixture of oligosaccharides containing 5 to 6 mannose units was the most effective inhibitor. Since it is known that these oligosaccharides contain a predominance of alpha 1-2 linkages and lesser numbers of alpha 1-3 linkages, it is likely that these are important in the structural configuration of the antigenic determinants.

Journal Article↗