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Biomedical subjects

H F Troutt

Publications and source records attributed to H F Troutt.

At least 19 recordsLinked to original sources
PubMed2001

Prevalence of Salmonella spp in cull (market) dairy cows at slaughter.

OBJECTIVE: To determine the prevalence of Salmonella spp in the cecal-colon contents of cull (market) dairy cows at slaughter because of potential public health ramifications. DESIGN: Survey study. SAMPLE POPULATION: Cecal-colon contents collected from 5,087 cull (market) dairy cows at slaughter at 5 slaughter establishments across the United States. PROCEDURE: During 2 periods of the year, winter (January and February) and summer (July through September), 5 cull (market) cow slaughter establishments in the United States--west (WE), southeast (SEE), central (CE), north central (NCE), and south central (SCE)--establishments were visited, and cecal-colon contents of cull dairy cows were obtained at the time of slaughter. Samples were examined by microbiologic culture at a single laboratory for Salmonella spp. RESULTS: Salmonella spp were detected in 23.1% of cecal-colon content samples from cull dairy cows across the 5 slaughter establishments. The highest site prevalence (54.5%) was detected at the WE during the summer period, whereas the lowest was found at the CE during the summer (4.3%) and at the NCE during the winter (4.5%). Considerable variation in the daily prevalence of Salmonella spp was found, particularly at the WE and the SCE. Salmonella spp were isolated from 93% of cecal-colon contents collected on a summer day at the WE. CONCLUSIONS AND CLINICAL RELEVANCE: Results strongly suggest that there is a high prevalence of Salmonella spp in cull dairy cows at slaughter, which could burden Hazard Analysis Critical Control Point programs implemented in slaughter establishments. Procedures to reduce Salmonella load at the dairy farm and during transport to slaughter could reduce the risk of spread during the slaughter process.

Abattoirs↗
PubMed2001

Diversity of Salmonella serotypes in cull (market) dairy cows at slaughter.

OBJECTIVE: To determine the diversity of Salmonella serotypes isolated from a large population of cull (market) dairy cows at slaughter. DESIGN: Cross-sectional study. SAMPLE POPULATION: Salmonella organisms isolated from the cecal-colon contents of 5,087 market dairy cows. PROCEDURE: During winter and summer 1996, cecal-colon contents of cull dairy cows at slaughter were obtained from 5 US slaughter establishments. Specimens were subjected to microbiologic culturing for Salmonella spp at 1 laboratory. Identified isolates were compared with Salmonella isolation lists published by the Centers for Disease Control and Prevention (CDC) and the National Veterinary Services Laboratory (NVSL) for approximately the same period. The Simpson diversity index was used to calculate the likelihood that Salmonella isolates selected randomly by establishment were different. RESULTS: Of 58 Salmonella serotypes identified, Salmonella ser. Montevideo was the most prevalent. Two of the top 10 CDC serotypes identified from in 1996, Salmonella ser. Typhimurium and S Montevideo, appeared on our top 10 list; 8 of the top 10 were found on NVSL listings. Thirty-one of 59 S. Typhimurium isolates were identified as DT104 and found at a west slaughter establishment, 30 during the winter and 1 during the summer. The greatest diversity of serotypes was at a southeast establishment during the summer; the least diversity was at a central establishment in the winter. CONCLUSIONS AND CLINICAL RELEVANCE: 58 Salmonella serotypes were isolated from market dairy cows at slaughter and could pose a threat for food-borne illness. Salmonella Montevideo was the most frequently isolated serotype and may contribute substantially to salmonellosis in dairy cattle.

Animals↗
PubMed2000

In vitro susceptibility of Yersinia enterocolitica isolated from the oral cavity of swine.

Antimicrobial susceptibility of 181 (107 ail-harboring isolates and 74 non-ail-harboring) Yersinia enterocolitica isolates obtained from the oral cavity of swine was determined against 24 antimicrobial agents. All Y. enterocolitica isolates were susceptible to sulfamethoxazole/trimethoprim, enrofloxacin, aminoglycosides, and nitrofurantoin. Susceptibility to tetracycline appeared to vary by lot of origin. Isolates were resistant to sulfonamides (other than sulfamethoxazole/trimethoprim), penicillin, ampicillin, ticarcillin, cephalothin, macrolides, and tiamulin.

Animals↗
PubMed1998

Prevalence of pathogenic Yersinia enterocolitica in groups of swine at slaughter.

In order to estimate the prevalence of swine herds infected with pathogenic Yersinia enterocolitica, 103 lots of market swine were randomly selected at slaughter during six 1-month intervals. Pigs within each lot were sampled by swabbing the oral-pharyngeal surface, poststunning and postexsanguination but prescalding. Ninety-five lots (92.2%) contained at least one pig infected with Y. enterocolitica. Pathogenic strains were defined as those harboring the ail gene which has been identified in Y. enterocolitica that causes human clinical disease. Identification of those strains harboring the ail gene was accomplished using a polymerase chain reaction technique. Twenty-nine lots (28.2%) contained at least one pig from which ail-containing (pathogenic) Y. enterocolitica were isolated. Of the 107 pathogenic Y. enterocolitica isolates identified, 89.7% were serotype O:5 and 3.7% were serotype O:3. The results from this study will aid in the design of future epidemiological investigations concerning on-farm prevalence and associated risk factors for pathogenic Y. enterocolitica. Additionally, the results support the hypothesis that swine are a significant potential reservoir for human infections by Y. enterocolitica.

Abattoirs↗
PubMed1997

Meat from dairy cows: possible microbiological hazards and risks.

The authors provide an overview of the circumstances associated with culling of dairy cattle in the United States of America (USA) and focus on the possible significant microbiological hazards associated with meat from cull dairy cows. Cull dairy cows are an important source of food in the USA, accounting for at least approximately 17% of ground beef. The potential microbiological hazards for foodborne illness from cull dairy cows discussed here include Salmonella (with special attention to S. Typhimurium DT104), Escherichia coli O157:H7, Campylobacter jejuni, Listeria monocytogenes, Clostridium perfringens and Staphylococcus aureus. Possible sources and means of contamination are pointed out, as are the potential foodborne risks from Bacillus cereus and Aeromonas spp. In conclusion, widespread microbiological studies are needed to determine the prevalence and risk of foodborne pathogens in cull dairy cattle.

Animals↗
PubMed1994

Bovine ketosis and somatotrophin: risk factors for ketosis and effects of ketosis on health and production.

Aspects of the metabolism and health of 63 cows which had been treated with different amounts of bovine somatotrophin (BST) daily in the preceding lactation and 25 control cows were studied. The aims of the study were first, to identify cows with ketotic conditions, either by measurements of blood metabolite concentrations or by clinical observations, secondly, to evaluate the risk of such conditions in cows treated with BST in the preceding lactation, and thirdly, to examine the metabolic and production consequences of the ketotic conditions in an environment in which the cows' health, body condition and nutrition were closely observed. The cows were categorised objectively by the use of cluster analysis into non-ketotic cows and ketonaemic cows, on the basis of their plasma metabolite concentrations. Twelve of the control cows and none of the cows previously treated with BST were classified as ketonaemic. Similarly, nine of the control cows but only two of the cows previously treated with BST had clinical ketosis. Some, but not all, of the decrease in the risk of clinical ketosis was attributable to the lower body condition score of the cows previously treated with BST. The clinically ketotic cows had a greater risk of other illness in the first 10 days post partum than their herdmates, but the ketonaemic cows had a significantly lower risk of other disease in the first 10 days post partum. The ketonaemic control cows were significantly heavier than the non-ketotic control cows, but they maintained a higher dry matter intake than the latter cows, probably a key factor in reducing the risk of clinical ketosis.

3-Hydroxybutyric Acid↗
PubMed1994

Pregnancy incidence in high producing dairy cows treated with recombinant bovine somatotropin.

Reproductive performance in cows exposed for two lactations to recombinant bST was measured using unconditional logistic regression. Intramuscular administration of 17.2, 51.6, and 86 mg of recombinant bST/d per cow started at d 70 postpartum and ended at dry-off or 305 d postpartum. Performance was measured as the cumulative incidence of cows becoming pregnant by 305 d post-partum. Within each parity group, cows treated with bST had higher total milk yield. During the first study lactation, the log odds of a cow becoming pregnant decreased linearly as dose increased, following adjustment for serum cholesterol, blood urea N, average daily milk yield, peak milk yield, net energy balance, and body condition score. However, differences in pregnancy incidence between treated and untreated cows were statistically significant only at the higher dose categories, the 51.6 and 86.0 mg of recombinant bST per cow. During the second study lactation, pregnancy rates were similar to those in the first lactation; rates reduced as recombinant bST dose increased. The singular exception was for cows exposed to recombinant bST during the previous lactation but not during the second. In the latter group, previously treated cows had higher pregnancy incidence than did the controls.

Animals↗
PubMed1994

Interval from calving to conception in high producing dairy cows treated with recombinant bovine somatotropin.

Intervals from calving to conception in cows treated for two lactations with recombinant bST were ascertained using survival analysis methods. Daily intramuscular administration of placebo, 17.2, 51.6, or 86 mg of bST per cow started at d 70 postpartum and ended at culling or drying off. Breeding began at d 70 postpartum. Multiparous cows treated during the first lactation had significantly longer intervals from calving to conception. Cows treated during the previous lactation, but not during the second lactation, had significantly shorter intervals from calving to conception. Cox proportional hazards regression analysis was used with adjustment for the confounding effect of serum metabolites, milk production, energy balance, and body condition.

Animals↗
PubMed1994

Reproductive performance in high producing dairy cows treated with recombinant bovine somatotropin.

The reproductive performance of 156 cows treated with recombinant bST for two consecutive lactations was assessed as part of a target animal safety study conducted in the San Joaquín Valley of central California. Daily intramuscular administration of 17.2, 51.6, and 86 mg of bST per cow started at d 70 postpartum and ended at dry-off or 305 d postpartum. The AI did not begin until d 70 postpartum. During the first lactation study, multiparous cows treated with bST had significantly decreased pregnancy rates, increased behavioral anestrus, and increased anestrus confirmed by palpation. Treated primiparous cows in first lactation had shorter mean days to first standing estrus. In the second lactation study, cows treated with bST had an increased rate of delayed uterine involution, cystic ovarian condition, behavioral anestrus, and anestrus confirmed by palpation. Progeny from the first lactation cows were evaluated for rates of growth, morbidity, mortality, and for reproductive performance; no difference was detected between the progeny from different groups.

Analysis of Variance↗
PubMed1992

Bovine somatotropin.

Bovine somatotropin has the potential to alter profoundly the way that dairy herds are managed and to change the ways in which veterinarians provide services to those herds. This article describes the structure, function, and action of somatotropin. The metabolic effects of somatotropin on dairy cattle are discussed.

Animals↗
PubMed1992

Time series cross-correlation analysis of postparturient relationships among serum metabolites and yield variables in Holstein cows.

Time series cross-correlation analysis was used to determine relationships among serum beta-hydroxybutyrate, glucose, FFA, cholesterol, milk yield, DMI, and estimated net energy balance for 42 d postpartum for 14 multiparous cows. Milk yield was positively associated with beta-hydroxybutyrate concentrations on the same day, and milk yield was a primary determinant of metabolic change. Dry matter intake was negatively correlated with beta-hydroxybutyrate concentrations 3 d later and on the same day. The data supported in vitro studies showing that FFA are positively associated with beta-hydroxybutyrate production, showed that glucose concentrations are negatively cross-correlated with beta-hydroxybutyrate concentrations, and found that estimated net energy balance is negatively cross-correlated with beta-hydroxybutyrate. Analyses suggested that serum glucose and cholesterol concentrations significantly decreased with increased milk yield; analyses also suggested that elevated beta-hydroxybutyrate concentrations were associated with decreases in milk yield 6 d later. beta-Hydroxybutyrate and FFA concentrations 3 to 9 d before parturition were positively related to cholesterol concentrations, and relationships were positive between estimated net energy balance and cholesterol, suggesting that cholesterol concentrations increased as precursors for cholesterol synthesis were available. Time series cross-correlation analysis was a useful tool in examining relationships among variables when repeated samples were obtained from the same individuals.

3-Hydroxybutyric Acid↗
PubMed1992

Impact of bovine somatotropin administration beginning at day 70 of lactation on serum metabolites, milk constituents, and production in cows previously exposed to exogenous somatotropin.

Metabolic and production responses are reported for 72 cows treated with bovine somatotropin (BST) for 30 days starting at day 70 of lactation. Of these 72 cows, 48 had been exposed in the preceding lactation to long-term treatment with BST at 3 dosages and 24 (controls) had not been given BST. Approximately half of the cows in each group were parity-2 cows, the rest were older. Comparisons between groups were made separately for parity-2, and older cows. Analyses, using pretreatment values of each variable as a covariate, indicated that older cows, but not parity-2 cows, significantly (P less than 0.05) increased milk production during treatment. Parity-2 cows, however, had a significantly higher milk fat percentage than controls following treatment. Cows treated with 51.6 or 86 mg BST/d in both parity groups had significantly higher serum-free fatty acids than controls. Estimated net energy balances were significantly lower for older treated cows, but did not significantly differ from controls for parity-2 treated cows. Older cows in the 86 mg of BST/d group tended to have higher concentrations of blood glucose than did older control-group cows. Treatment with BST did not significantly increase serum ketone concentrations in any group of animals, and none of the cows developed clinical ketosis during this period. Estimated net energy balance (ENEB) during treatment was a significant (P less than 0.05) covariate for free fatty acid concentrations in older cows and for milk fat percentage in parity-2 cows. Covariate adjusted analyses, using ENEB during treatment as a covariate, indicated that lipolytic stimuli already acting may be enhanced by treatment with BST, but a negative energy balance was not a necessary precondition for free fatty acid concentrations to increase following somatotropin treatment. Similarly, milk fat percentages for parity-2 treated cows were significantly (P less than 0.05) higher during treatment than controls when ENEB during treatment was used as a covariate. Increased milk fat concentrations in parity-2 treated cows were not associated with significant increases in the ratio of C18:C4-10 milk fatty acids, indicating that increased milk fat resulted from either an increase in incorporation of C18 fatty acids into milk fat coupled with an increase in de novo mammary synthesis of C4-10 milk fatty acids or an increase in C12-16 fatty acids that may arise either from increased tissue mobilization, from diet, or from de novo mammary synthesis.

Animals↗
PubMed1991

Postparturient metabolic and production responses in cows previously exposed to long-term treatment with somatotropin.

Postparturient metabolic and production responses were studied for 85 multiparous cows consisting of controls and animals that had been previously exposed to long-term treatment with bST. Older cows previously exposed to bST at high doses (51.6 mg/d) had lower milk yields than controls. However, there were no significant differences compared with controls in milk yield for lower dose (17.2 mg/d) older cows or parity 2 cow groups. The bST-treated groups had higher postpartum DMI, lower serum FFA, lower serum beta-hydroxybutyrate levels, and higher serum glucose than controls. Some bST-exposed groups had lower milk fat but higher milk protein levels. All bST-treated groups had significantly lower ratios of C18 to C4 to 10 milk fatty acids. Control cows of both parity groups were in higher body condition and weighed more than bST-treated cows prior to calving. The differences in postpartum metabolism may be attributed to differences in body composition resulting from bST treatment in the prior lactation, because use of body condition score prior to calving as a covariate did not substantially alter responses. The control cows probably had greater reserves of mobilizable lipid. These contentions are supported by changes in milk composition for bST-treated cows, which were consistent with animals of greater appetite, mobilizing less lipid. The bST-treated cows were probably more energetically efficient milk producers because they produced milk from feed rather than from mobilization of tissue stores. These data suggest that bST use in a prior lactation may have potential benefit in reducing risk of metabolic disorders associated with lipid mobilization in the postparturient period.

3-Hydroxybutyric Acid↗
PubMed1990

An investigation of bulk tank milk selenium levels in the San Joaquin Valley of California.

We evaluated selenium determination of bulk milk tank samples as an alternative to testing blood selenium for evaluating herd selenium status in DHIA dairy herds in the San Joaquin Valley of California. A method of determining milk selenium levels using inductively coupled plasma spectrometry is described. Mean bulk tank milk selenium levels were 0.0224 mg/L (Range 0.0126-0.0418 mg/L). No statistically significant relationships were found between bulk tank milk selenium levels of a herd and calving interval, days open or log somatic cell counts. Mean herd blood and milk levels were directly proportional to bulk tank milk selenium levels. Within a herd milk selenium levels of a cow were directly proportional to the cow's blood selenium level. Herd selenium levels were not significantly related to soil selenium levels. Determination of bulk tank milk selenium levels has the potential to be a low cost, non-invasive means of evaluating herd selenium levels in order to determine selenium deficiency. Further studies with this technique in areas which are deficient in selenium may provide estimates of the sensitivity, specificity and predictive value of bulk milk tank selenium for determining selenium deficiency in dairy herds.

Analysis of Variance↗
PubMed1988

Effects of hypocalcaemia on abomasal motility.

Abomasal mechanical and myoelectric activity were measured in two adult Holstein cows. Hypocalcaemia was induced using a constant infusion of disodium EDTA and serum total and diffusible calcium concentrations were measured. Abomasal motility remained normal in the face of declining calcium concentrations until a threshold value was reached (1.2 +/- 0.2 mmol litre-1) below which all mechanical and electrical activity was absent. A similar threshold effect was observed when calcium concentrations were allowed to rise. These data do not support hypocalcaemia as being a clinically important cause of abomasal atony.

Abomasum↗
PubMed1986

Iatrogenic lidocaine toxicosis in ewes.

Lidocaine intoxication developed inadvertently in a group of ewes. Six ewes used in a student laboratory session on laparotomy procedures were anesthetized locally with lidocaine hydrochloride (20 mg/kg) prior to simultaneous bilateral laparotomy. Shortly after the administration of lidocaine hydrochloride, the ewes developed muscle tremors and became recumbent, with a variety of neurologic signs including dullness, opisthotonos, odontoprisis, mydriasis, blindness, extensor rigidity, and convulsions. The ewes were given intravenous fluid therapy, and 90 minutes after the onset of signs, the ewes were standing, dull, and appetent. The laboratory session was rescheduled 2 weeks later, using the same ewes and a reduced dosage of lidocaine hydrochloride (10 mg/kg). There were no adverse effects and the simultaneous bilateral laparotomy procedure was completed without complications. The authors recommend the use of lidocaine hydrochloride at a dosage not exceeding 10 mg/kg for ewes requiring extensive local anesthesia.

Animals↗
PubMed1985

Lesions of spinal cord parelaphostrongylosis in sheep. Sequential changes following intramedullary larval migration.

Spinal cord nematodiasis epidemiologically, clinically, and histologically consistent with Parelaphostrongylus tenuis infection was noted in two flocks of sheep. Spinal cords from two sheep with active infection and one from a partially recovered animal were studied in an effort to determine the sequence of lesions following larval invasion of the central nervous system. In the former two sheep, migration of larvae within the spinal cord induced asymmetrically irregular tracks of disrupted and necrotic tissue, primarily in white matter. Subsequently, macrophages infiltrated these regions and phagocytized the necrotic tissue, which led to cavity formation. Swelling and loss of axons, diminished myelin staining, mononuclear cell infiltration and increase in astrocytic fibers were often seen in adjacent tissue. Only occasional coiled larvae were found in these actively infected animals. Late stage lesions in the white matter in the partially recovered sheep included multiple small astrogliotic regions with diminished myelin and axonal content, and a single large multicavitary, atrophic, gliotic zone.

Animals↗