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H Feldmeier

Publications and source records attributed to H Feldmeier.

142 records · Page 8Linked to original sources
PubMed1977

[Gluconeogenesis in perfused rat liver following preservation in various temperature ranges].

Experiments with perfused rat livers have shown that rates of various metabolic processes are influenced by changes in temperature in different ways. Cytosolic processes exhibit a linear temperature dependence when plotted in an Arrhenius-diagram in the range between 37 degrees and 1 degree C. In contrast, processes which are bound to mitochondrial membranes show phase changes at 22 degrees C. Furthermore, with gluconeogenesis another phase was observed at 11 degrees C. After 24 hour of perfusion at 11 degrees C the livers produced more glucose from gluconeogenic precursors when perfused at 37 degrees C than after perfusion at 4 degrees C. The importance of the balance of metabolism during hypothermia is discussed by comparison with metabolism of poikilothermic animals.

Animals↗
PubMed1976

[Electronic data processing in medical microbiology--a guide and some critical thoughts].

Applications of electron data processing in all fields of medicine and especially in medical microbiology are increasing rapidly. It seems important to discuss a guide for using such complicated electronical systems, because, on the one hand, computer applications in medicine are desirable for scientific reasons, but on the other hand few very costly and sophisticated projects had to be cancelled, after a shorter or longer time of implementation by reason of lack of co-operation of medical collaborators. This paper puts on discussion such a guide for using electronic data processing in medical microbiology. It is proposed to put through the following tasks before introducing a computer system into a microbiological institution: a system analysis of the status quo, an objective determination of the set state, a system design and a cost-benefit analysis. Those tasks are described in detail and are illustrated to some extent by graphics and tables from own research for preparing a data processing system for medical microbiology. It is emphasized that beside hard- and software criteria of the desired computer system one has to pay attention to the problems of man-machine engineering. The paper finishes with concrete propositions of proceeding when the computer system is implemented and shows possibilities of scientific data evaluation of a microbiological data base.

Costs and Cost Analysis↗
PubMedDate not supplied

Differences in drug response of Plasmodium falciparum within an area of the Amazon region.

Clinical characteristics of patients with falciparum malaria, as well as sensitivity of Plasmodium falciparum to chloroquine and mefloquine, were investigated in 2 distinct strata within the same geographical area of the Amazon Basin. One stratum was the population living along the road, the other that living along the river, both near Rio Branco, capital of Acre State, Brazil. The clinical features did not differ between the 2 strata. Full in vitro sensitivity of P. falciparum to mefloquine was observed in both areas. However, significant differences in chloroquine sensitivity were observed between the 2 strata. EC50 values for chloroquine were 0.8484 mumol/litre for parasite isolates from the road stratum (E) and 0.4638 mumol/litre for parasite isolates from the river stratum (R). EC90 values were 2.8095 and 1.2549 mumol/litre in strata 'E' and 'R', respectively. Continuous drug pressure over years in area 'E' and relatively low drug pressure in area 'R' were presumably responsible for these differences.

Animals↗
PubMedDate not supplied

Detection of the schistosome circulating cathodic antigen by enzyme immunoassay using biotinylated monoclonal antibodies.

We have developed an enzyme immunoassay (ELISA) for the quantification of the schistosome circulating cathodic antigen (CCA), a glycoprotein associated with the syncitium lining the gut of the parasite. A mouse monoclonal antibody of IgG3 isotype was used as coating (antigen-capture) antibody, while a biotinylated mouse monoclonal IgM was used as second (antigen-detecting) antibody. Streptavidin-alkaline phosphatase was used as enzyme label. The lower detection limit of the assay was 1.0 ng of the trichloroacetic acid soluble fraction of adult worm antigen (AWA-TCA) per ml, which corresponds to approximately 0.2 ng CCA per ml. The ELISA showed a linear range from 1.0 to 62.5 ng AWA-TCA per ml. Serum and urine samples of 16 individuals infected with Schistosoma mansoni (egg counts ranging from 5 to 4820 eggs per gram of faeces) were tested in the assay. Antigen titres ranged from less than 4-8192. This assay represents a considerable advantage in diagnosis of Schistosoma infections as it allows the detection and quantification of CCA in serum and urine in even lightly infected individuals.

Animals↗
PubMedDate not supplied

Praziquantel in the treatment of hepatosplenic schistosomiasis: biochemical disease markers indicate deceleration of fibrogenesis and diminution of portal flow obstruction.

Serological indicators for disease activity and portal vein obstruction and/or deviation were assessed in 23 patients with hepatosplenic schistosomiasis before, and up to 18 months after, praziquantel treatment, as well as in 25 matched local controls. Cessation of egg-induced immunopathology was reflected by the return to normal of serum procollagen-III-propeptide and neopterin concentrations. Reversibility of portal vein pathology was indicated by normal clearance of cholylglycine in cases without signs of decompensating portal hypertension. In most patients with a history of ascites and/or haemorrhage, serum cholylglycine concentration remained pathological. The results provide evidence that the fibrogenic process ceases after specific chemotherapy, and that portal vein pathology regresses in a substantial proportion of hepatosplenic schistosomiasis cases.

Adolescent↗
PubMedDate not supplied

Quantitative determination of circulating anodic and cathodic antigens in serum and urine of individuals infected with Schistosoma intercalatum.

Circulating anodic and cathodic Schistosoma antigens (CAA and CCA) have been determined by enzyme immunoassays in serum and urine of 60 individuals infected with S. intercalatum in Equatorial Guinea. The median egg output was 29 eggs/g of faeces (range 3-840). The egg output strongly correlated with concentrations of serum CAA (p = 0.47) and urine CAA (p = 0.42) (P < 0.001 for both); the later 2 quantities were also correlated with each other (p = 0.44, P < 0.001). All except 3 infected individuals had detectable amounts of serum CAA and/or urine CCA, a sensitivity of 95% for these 2 tests combined. Urine CAA was detected in 43% of patients. Serum CCA was detected in all infected individuals; however, no significant correlation was obtained between serum CCA levels and egg output in the stools of individual patients. This is the first study to demonstrate CCA in specimens of patients infected with S. intercalatum. The detection of CCA in urine is a new, non-invasive diagnostic method for S. intercalatum infection.

Adolescent↗
PubMedDate not supplied

Female genital schistosomiasis as a risk-factor for the transmission of HIV.

Sexually transmitted diseases increase the probability for HIV transmission, presumably through lesions in the genital mucosa. Female genital schistosomiasis, a special form of urinary schistosomiasis due to infection with Schistosoma haematobium, may be another risk-factor for transmission of HIV. From published data there seem to be pathophysiological, immunological and epidemiological evidence for an association between genital ulcer disease due to S. haematobium and HIV-infection in women. Female genital schistosomiasis could be seen as an example of how an interaction between a parasitic disease and HIV facilitates the propagation of the latter. As long as the prevalence of HIV is low in the general population, interventions targeted to high risk groups will significantly delay, or even prevent, widespread dissemination of the HIV infection in the rest of the population. If female genital schistosomiasis is a risk factor for the spread of HIV like other genital ulcer diseases, there should be interesting ways to intervene from the public health point of view.

Adolescent↗