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Biomedical subjects

H Fey

Publications and source records attributed to H Fey.

18 recordsLinked to original sources
PubMed1979

Antibody coated bacteria in urine sediment from cattle infected with Corynebacterium renale.

Using the fluorescent antibody test, the presence of antibody-coated bacteria in 10 out of 17 urine sediment samples from cattle infected with Corynebacterium renale is described. These antibodies were mainly of the immunoglobulin class IgG, and to a lesser extent IgA. This finding is characteristic for infections of the upper urinary tract (pyelonephritis). In seven samples no antibody coating of the bacterial surface was detected. In these cases an infection of the lower urinary tract (cystitis) is suggested.

Animals↗
PubMed1979

A novel method for the production of Salmonella flagellar antigen. II. Further purification for the preparation of H antisera.

A method for a simple preparation of Salmonella flagellar antigen is described. The antigen is sufficiently pure to elicit high titered H antibodies of 12,800-51,200 and O titers of less than 50. Highly motile Salmonella test strains are grown on 0.8% swarm agar and harvested with 0.05 n HCl which solubilizes the flagella. The suspension with a pH of 1.5 is kept at 4 degrees C. over night and then centrifuged at 49,000 g for 60 min. The supernatant is neutralized and precipitated with ammonium sulfate at 2/3 saturation. The resulting polymeric flagellin is submitted to a zone electrophoresis on Pevikon. Strips are cut from the "cake" and eluted. The H antigen is found on the anodic side, the O antigen remains near the trough or migrates slightly cathodically. Form 20 plates enough flagellin is collected fro the immunization of 50-100 rabbits. The Latex test proved to be especially suited for the checking of the H antigen.

Animals↗
PubMed1978

Simple assay for staphylococcal enterotoxins A, B, and C: modification of enzyme-linked immunosorbent assay.

The enzyme-linked immunosorbent assay (ELISA) introduced for the detection of staphylococcal enterotoxins by Saunders et al., Simon and Terplan, and ourselves has proved to be a simple, reliable, and sensitive test. A new modification is described that uses polystyrene balls (diameter, 6 mm) coated individually with antibody against one of the toxins A, B, or C. In a single tube, 20 ml of the food extract was incubated with the three balls differently stained, which were then each tested for the uptake of enterotoxin by a competitive ELISA. A concentration of 0.1 ng or less of enterotoxin per ml can be measured, making tedious concentration procedures of the extracts superfluous. Culture supernatants and extracts from foods artificially or naturally contaminated with toxin were successfully examined. Cross-reactions did not occur, and nonspecific interfering substances did not create serious problems.

Enterotoxins↗
PubMed1978

An economic and rapid diagnostic procedure for the detection of salmonella/shigella using the polyvalent salmonella phage O-1.

An easy, rapid and economic two-step procedure is described for the detection of Salmonella/Shigella. In the first step the susceptibility of suspected colonies for the phage O-1 of FELIX and CALLOW is tested. Positive cultures are serologically confirmed. The test is performed on Triple Sugar Iron Agar and lasts 4-6 hrs. Phage negative cultures which are lactose- and sucrose negative are tested for lysine decarboxylase and, if Shigella is possible (i.e. in human material on primary plates), for indol production and motility in a semisolid tryptophane agar. Of 22880 Salmonella straine 21977, i.e. 96.1% were phage-sensitive. Strains belonging to certain O-groups (OE) or species are lysed at a lower percentage. However, since they are lysine decarboxylase positive they are not lost and can be submitted to a serological examination.

Carboxy-Lyases↗
PubMed1977

[Measurement of human tetanus antitoxin using as enzyme-linked immunosorbent assay].

An enzyme-linked immunosorbent assay (ELISA) has been developed for the detection and measurement of human tetanus antitoxin. This simple test has proved to be a simple and sensitive as the radio immunosorbent test (RIST) described earlier by the authors and needs no special equipment. The technique which is based on the pioneer work done by Eva Engvall, is described. The antitoxin titers obtained with ELISA and RIST are comparable.

Antibody Formation↗
PubMed1975

[Radioimmunological measurement of tetanus antitoxin].

A radioimmunological assay method has been developed for large-scale screening for tetanus antibodies. Its specificity is excellent and the sensitivity is in the region of 0.001 International Units per ml; it is thus comparable with the animal protective test. By this method a series of human sera was examined for which the vaccinal antitoxin titers were determined by animal protection tests. A parallel between the two tests was confirmed. Screening of 60 military recruits established that the test is usable as a mass method. Furthermore, the technique of Habermann and Wiegand was compared with ours and the results correlated well.

Absorption↗
PubMed1975

Production of potent salmonella H antisera by immunization with flagellae, isolated by immunosorption.

For serotyping Salmonella H sera should exhibit a high H titer and preferably a low O titer which does not interfere with the H slide agglutination at the working dilution of the serum. By conventional immunization with motile bacteria OH sera are always produced. We isolated flagellae from highly motile Salmonella cultures, which were detached from the bacterial cells by a waring blendor, with an immunosorbent, i.e. cyanobromide-activated Sepharose to which the respective anti H antibody was coupled. The desorption of the flagella from the immunosorbent was done by lowering the pH to 2.3 with glycine-HC1 buffer. 4.2 mg of flagellar protein were recovered from 20 agar swarm plates. 10 mug of the antigen incorporated into complete Freund adjuvant was found to immunize a rabbit successfully. The H titers varied from 10,000-160,000 and the majority of the O titers did not exceed 160. Only one immunosorbent was necessary to isolate a group of related H antigens (i.e., lv, lw, lz13, lz28). After regeneration the immunosorbent can be reused many times.

Adsorption↗